Labshake search
Citations for R&D Systems :
1901 - 1950 of 2242 citations for CEACAM 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... the slides were incubated in 5 μg/mL solution of anti-SOX2 antibodies (R&D Systems) or 5 μg/mL solution of anti-Sox17 (SantaCruz ...
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bioRxiv - Immunology 2022Quote: ... α-ULPB2/5/6 (mouse, clone 165903) and α-CCR4-APC (clone 205410) from R&D Systems. α-mouse IgG-APC from Southern Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA) or human IgG isotype control (#31154 ...
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bioRxiv - Cell Biology 2020Quote: ... Replated cultures of BMDMs were primed with murine interferon-γ (5 ng/mL, R&D Systems) over night and again two hours before stimulation with MDP (10 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... cell lysates (100 μg of protein) were incubated with O-GlcNAcase (5 μg, R&D Systems) at 37°C for 2 h.
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bioRxiv - Neuroscience 2022Quote: ... 5 μg/ml of TGF-β RII Fc chimera (TGF-β RII Fc) (R&D Systems), was applied to SN cultures 1 h prior to fixation to inhibit TGF-β signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... the media were supplemented with 5 ng/mL TGF-β1 (R&D Systems 7754-BH/CF) for 7 days.
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bioRxiv - Bioengineering 2023Quote: ... B cell activating medium was supplemented with 5 ug/mL anti-CD40 antibody (R&D systems) and 20 ng/mL IL-4 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... Quantitative analysis for IL-5 was performed using enzyme-linked immunosorbent assay (ELISA) (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 5 µl/0.5 µM Ubiquitin-AMC (R&D Systems #U-550-050) substrate ...
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bioRxiv - Molecular Biology 2020Quote: L-FABP levels in human and mouse sera were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Epidemiology 2019Quote: ... HGF concentration in rinse water samples was measured by using HGF ELISA kit (Quantikine human HGF immunoassay, R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Plasma IL-6 was assessed in duplicate using high-sensitivity enzyme-linked immunosorbent assay (ELISA; Quantikine HS Human IL-6 ELISA, R&D Systems, UK) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Soluble protease expression profile of MRC-5 and hTERT-HGF 2D and 3D conditions were performed using a Human Protease Array kit according to manufacturer’s guidelines (R&D Systems, Minneapolis, MI). Images were acquired on a LAS-4000 Fujifilm imager and analyzed after subtraction of background using “Protein Array Analyzer” plugin in (Carpentier and Henault ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme-linked immunosorbent assay for human LTα was performed in triplicate using the DUO set ELISA kit (DY210, R&D Systems, Abingdon, UK). Protein was extracted from transfected meningeal cells using RIPA buffer (R0278 ...
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bioRxiv - Neuroscience 2021Quote: ... The contribution of BDNF/TrkB signaling was tested by bath application of a scavenger of endogenous BDNF (recombinant human TrkB-Fc chimera, R&D Systems, Germany). For scavenging of BDNF ...
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bioRxiv - Neuroscience 2021Quote: The relative phosphorylation levels of various kinases were measured using a Proteome Profiler Array human phospho-kinase array kit (R&D Systems #ARY003C) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1, 1:50,000, R&D Systems – AF3787). After 2x10 minutes washes with milliQ water and 2x10 minutes washes with TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of 105 proteins in supernatants was evaluated using human cytokine and chemokine XL proteome array kit from R&D Systems (Minneapolis, MN).
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bioRxiv - Cancer Biology 2020Quote: ... Then we collected the supernatants and cell lysates so that we could detect the chemokines using a Proteome Profiler Human Chemokine Array Kit (R&D Systems, ARY017).
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bioRxiv - Immunology 2022Quote: ... Detection of bound sIL7R was carried out by one-hour incubation at room temperature with biotinylated goat anti-human IL7R polyclonal antibody (R&D Systems, # BAF306), followed by 30-minute incubation at room temperature with streptavidin-horseradish peroxidase (Millipore Sigma ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted and resuspended in 100 µL FACS buffer (2% FBS in DPBS) containing 0.2 µg goat-α-human Ace-2 (R&D Systems #AF933). After 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: CCL22/Ccl22 ELISAs were performed on culture supernatants (undiluted or diluted when necessary) using Immulon 4HBX flat bottom microtiter plates with the human CCL22/MDC DuoSet ELISA (R&D Systems DY336) or the mouse Ccl22/Mdc DuoSet ELISA (R&D Systems DY439 ...
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bioRxiv - Cancer Biology 2020Quote: Angiogenesis secreted mediators were identified from fallopian tube secretory cells and ovarian carcinoma cells conditioned media employing the Human Angiogenesis Antibody Array (R&D systems: ARY007). ELISA was employed for the precise quantification of VEGF (R&D systems ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... to remove debris and processed according to the manufacturers’ instructions (Proteome Profiler™ Human XL cytokine array kit; R&D Systems, Abingdon, UK). In total ...
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bioRxiv - Cancer Biology 2022Quote: ... with good affinity and specificity were screened and selected from the homemade human single-chain antibody library through commercial VEGF and EGFR proteins (R&D Systems, Inc.). Then ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Cancer Biology 2019Quote: ... CCN4 concentration in conditioned medium was determined by ELISA using Human WISP-1/CCN4 DuoSet ELISA Development Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Fc Tag 15μg/ml (CDO-H5257, Acro Biosystem Newark, USA) or Recombinant Human IgG1 Fc 3μg/ml (110-HG, R&D Systems, Minneapolis, MN, USA) as control ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 1μg of mouse serum IgG for 10 min at RT then incubated with: AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), APC mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated in PBS-T with 1% ELK for 45 minutes with either goat anti-human C1r (1:300, R&D Systems, AF1807), sheep anti-human C1s (1:300 ...
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bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
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bioRxiv - Immunology 2020Quote: Cytokine and Chemokine secretion was assessed as previously described (van Wilgenburg et al., 2013) using the Proteome Profiler Human Cytokine Array Kit (R&D systems, #ARY005B). TNFα was quantified by ELISA (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Human Discovery Assay (R&D Systems). Custom configured targets include ...
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bioRxiv - Bioengineering 2020Quote: ... or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647, clone H398, lot 0410; or APC-labelled anti-mouse gp130, R&D Systems, cat #FAB4681A ...
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bioRxiv - Bioengineering 2022Quote: ... The concentration of hCG-β in the culture medium was measured using the Human CG beta (HCG beta) Duoset ELISA (#DY9034-05, R&D Systems) according to the manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
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bioRxiv - Immunology 2022Quote: Detection of secreted IL11 into the supernatant of HPAEpiC cultures was performed using the human IL-11 ELISA kit (R&D systems, D1100) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatants were collected after the second treatment and TNF-α levels were quantified by ELISA using the Human TNF-alpha DuoSet ELISA (R&D systems) following manufacturer recommendations.
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.