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Citations for R&D Systems :
2051 - 2100 of 2868 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were pelleted and resuspended in 100 µL FACS buffer (2% FBS in DPBS) containing 0.2 µg goat-α-human Ace-2 (R&D Systems #AF933). After 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: CCL22/Ccl22 ELISAs were performed on culture supernatants (undiluted or diluted when necessary) using Immulon 4HBX flat bottom microtiter plates with the human CCL22/MDC DuoSet ELISA (R&D Systems DY336) or the mouse Ccl22/Mdc DuoSet ELISA (R&D Systems DY439 ...
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bioRxiv - Immunology 2022Quote: ... to remove debris and processed according to the manufacturers’ instructions (Proteome Profiler™ Human XL cytokine array kit; R&D Systems, Abingdon, UK). In total ...
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bioRxiv - Cancer Biology 2022Quote: ... with good affinity and specificity were screened and selected from the homemade human single-chain antibody library through commercial VEGF and EGFR proteins (R&D Systems, Inc.). Then ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Cancer Biology 2019Quote: ... CCN4 concentration in conditioned medium was determined by ELISA using Human WISP-1/CCN4 DuoSet ELISA Development Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
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bioRxiv - Physiology 2021Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Fc Tag 15μg/ml (CDO-H5257, Acro Biosystem Newark, USA) or Recombinant Human IgG1 Fc 3μg/ml (110-HG, R&D Systems, Minneapolis, MN, USA) as control ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 1μg of mouse serum IgG for 10 min at RT then incubated with: AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), APC mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated in PBS-T with 1% ELK for 45 minutes with either goat anti-human C1r (1:300, R&D Systems, AF1807), sheep anti-human C1s (1:300 ...
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bioRxiv - Immunology 2020Quote: Cytokine and Chemokine secretion was assessed as previously described (van Wilgenburg et al., 2013) using the Proteome Profiler Human Cytokine Array Kit (R&D systems, #ARY005B). TNFα was quantified by ELISA (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Human Discovery Assay (R&D Systems). Custom configured targets include ...
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bioRxiv - Bioengineering 2020Quote: ... or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647, clone H398, lot 0410; or APC-labelled anti-mouse gp130, R&D Systems, cat #FAB4681A ...
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bioRxiv - Bioengineering 2022Quote: ... The concentration of hCG-β in the culture medium was measured using the Human CG beta (HCG beta) Duoset ELISA (#DY9034-05, R&D Systems) according to the manufacturer protocol.
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bioRxiv - Immunology 2022Quote: Detection of secreted IL11 into the supernatant of HPAEpiC cultures was performed using the human IL-11 ELISA kit (R&D systems, D1100) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatants were collected after the second treatment and TNF-α levels were quantified by ELISA using the Human TNF-alpha DuoSet ELISA (R&D systems) following manufacturer recommendations.
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.
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bioRxiv - Neuroscience 2023Quote: ... the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL recombinant human basic FGF (#233-FB, R&D Systems, Germany) and 10 µM Y-27632 (only for the first 24 h after passaging) ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were used to quantify the amount of cyto/chemokines produced by the urothelial organoids before and after infection using a human premixed multi-analyte customized kit in a Luminex® bead based immunoassay (R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA; R&D Systems) and/or 50 ng/ml IFN-γ (285-IF ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Immunology 2023Quote: ITCH ubiquitination reactions were carried out using the manufacturer’s protocol for the Human ITCH/AIP4 Ubiquitin Ligase Kit (R&D Systems, K-270). 10X reaction buffer ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 5 mM beta-glycerophosphate and 1% insulin-transferrin-selenous acid (osteogenic media) and stimulated them the next day with recombinant human BMP2 (300 ng/ml) (R&D Systems, MN) in serum-free osteogenic media ...
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bioRxiv - Biochemistry 2023Quote: ... and 250 μg of protein from each condition was incubated with a membrane array from the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957; iNOS, Abcam, Cat# ab15323; CD206, R&D Systems, Cat# AF2535) in TBST containing 2% BSA for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using the following primary and secondary antibodies: anti-IL-1β (R&D systems, AF-201-NA), anti-GFP (Clontech ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Developmental Biology 2019Quote: ... blocked (5 % BSA and 0.1 % Tween 20 in 1X TBS) for at least 1 hour at room temperature and stained with primary antibodies (Phospho-STAT3 Y705 and STAT3, R&D Systems 9131S and 9132S) diluted in blocking solution overnight at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... transferred to a fresh reaction tube and subsequently washed three times with 500 μl PBS-T before addition of primary antibody (R&D systems, Minneapolis, MN, USA) 1:5000 in 200 μl sample buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... for 24 hours and rested overnight in C-RPMI with 100µM ferric ammonium citrate (FeAC) and 200U/ml of human recombinant IFN-γ (R&D Systems, MN USA) unless otherwise stated.
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Biochemistry 2021Quote: Interleukin (IL)-6 in the patient plasma samples was measured by the Quantikine ELISA kit for human IL-6 (R&D Systems, Minneapolis, MN), as previously [29] ...
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bioRxiv - Microbiology 2020Quote: ... Ad-transduced cells were incubated for 24h before performing flow cytometry staining for surface expression of hACE2 using goat anti-human mAb AF933 (R&D Systems, Minneapolis, MN) used at a final concentration of 10μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Molecular Biology 2020Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Performance Assay Human High Sensitivity Cytokine Base Kit A (R&D Systems, Inc). Apical washes were diluted 1:2 ...
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bioRxiv - Cancer Biology 2022Quote: ... miniloci in an endogenous IgH and Igκ knockout background (19,20) were immunized with recombinant human LAG-3–Fc protein (hLAG-3–hFc) (R&D Systems, Minneapolis, MN, USA), consisting of the extracellular domain of LAG-3 (Leu23-Leu450 ...