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Citations for R&D Systems :
2301 - 2350 of 3037 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The supernatants were diluted when necessary to be in the optimal linear optical range and analyzed using commercial kits for IFNγ (Human IFN-gamma DuoSet ELISA, R&D Systems, USA and Bio legend, UK), and IL-10 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: Islet MTs were cultured for 1 day (short-term) or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... were washed and resuspended in PBS with 0.02% Tween-20 (PBS-T) and loaded with recombinant human IL13Rα2-Fc chimera (R&D Systems Cat#614INS; 2.1 µg per 3.9×107 beads) by incubation for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
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bioRxiv - Microbiology 2023Quote: Catalytic activities of SpyCEP constructs were measured through detection of remaining intact CXCL8 or CXCL1 substrate following incubation with SpyCEP by ELISA (R&D Systems human CXCL8 and CXCL1 DuoSet ELISA) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Murine CD3 T cells were isolated using Mouse CD3+ T Cell Enrichment Columns (R&D Systems) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic CD3+ T cells were isolated using CD3+ T cell enrichment columns (R&D Systems, MTCC25). T cells were cultured at 37C in RPMI-1640 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in B cell Excellerate medium with the CEllXVivo B cell expansion kit (R&D systems, UK). CD8+ T-cells (Cambridge Bioscience ...
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bioRxiv - Pathology 2023Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: GFP positive choroidal endothelial cells (ICEC2-TS) were cultured in complete endothelial cell growth media (R&D systems) containing 1% primocin (InvivoGen ...
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bioRxiv - Bioengineering 2020Quote: ... endothelial cell growth supplement (R&D Systems), L-glutamine and antibiotics (complete culture medium) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended to 1 x 105 cells/mL and mixed with 1% low-melting agarose (R&D Systems) at a 1:10 ratio and plated on 2-well comet slides (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were plated at 107 cells/well and differentiated with Dendritic Cell Differentiation Kit (cat# CDK004, R&D Systems) according to the manufactures instructions ...
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... PTPN14 (R&D Systems or Cell Signaling Technology), KRT1 (Santa Cruz) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with Wnt3a (R&D Systems), Noggin (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: mOSE cells (1×10^6 cells) were plated 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). RNA was collected 7 days post-TGFB1 treatment (RNAeasy Kit ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were differentiated into macrophages and dendritic cells using 50 ng/ml GM-CSF (R&D Systems, https://www.rndsystems.com) + 50 ng/ml M-CSF (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were then purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II and EasySep™ Magnet.
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bioRxiv - Genomics 2021Quote: ... and cells were labelled with phycoerythrin (PE)-conjugated antibody against the cell surface marker FGFR3 (FAB766P, clone 136334, R&D systems). Spermatogonial cells were isolated using a FACSAria II cell sorter (BD bio- sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 96 wells-plate were used to plate the cells at 10,000 cells/well in RPMI +10% FBS supplemented or not by different concentrations of recombinant Tpo (R&D Systems). After 72 hours ...
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bioRxiv - Developmental Biology 2023Quote: Surface expression of ITFG1 on cells was examined by staining the cells with mouse anti-ITFG1 antibody (R&D Systems, MAB89001) at 4°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Cancer Biology 2023Quote: ... CTSD for mouse cell lysates (#MAB1123-SP) and CTSA for mouse cell lysates (AF1029-SP) were bought from R&D Systems.
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bioRxiv - Cell Biology 2020Quote: ... Once the cells were adherent the cells undergoing differentiation were cultured in basal medium supplemented with 1% v/v Adipogenic Supplement (R&D Systems) while the control cells not undergoing differentiation were cultured in basal medium ...
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bioRxiv - Biochemistry 2022Quote: ... Cell identities were confirmed by satellite tandem repeat profiling and cells were tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2019Quote: ... Selected cytokine targets were measured in cell culture supernatants and cell lysates with commercially available Luminex® Multiplex Assays (R&D Systems) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells in ATP depletion were prepared by culturing the cells in a medium containing 100 μM CCCP (R&D systems, Minneapolis, MN) and 600 μM 2-deoxy-D-glucose (2DG ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Cell Biology 2024Quote: ... a suspension of 25,000 freshly dissociated cells or culture-expanded/FACS-sorted cells ranging from 625 to 5000 cells were prepared in growth factor reduced Matrigel (R&D Systems) and plated in 96-well plates as domes ...
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bioRxiv - Immunology 2023Quote: ... cells were initially activated at 5 x 105 cells/mL in B cell activation media: 5 µg/mL soluble CD40L (R&D Systems), 10 µg/mL anti-His tag antibody (clone AD1.1.10 ...
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bioRxiv - Immunology 2024Quote: ... Hematopoietic progenitor cells (HPC) were collected using Lineage Cell Depletion kit (Miltenyi Biotec, Bergisch Gladbach, Germany or R&D Systems, Minneapolis, MN). Then HPC were cultured in complete RPMI 1640 media with 10 ng/mL M-CSF (PeproTech ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Naïve T cells were isolated by negative selection using a Mouse Naive T Cell CD4+/CD62L+/CD44low Column Kit (R&D Systems). Naïve CD4 T cells were treated with fluorescein isothiocyanate-labeled (FITC ...
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bioRxiv - Cell Biology 2019Quote: ... cell were treated with mouse Sema3a (R&D systems) diluted in growth media for 5 minutes at 1nM ...
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bioRxiv - Genetics 2020Quote: ... glial cell-derived neurotrophic factor (GDNF) (R&D Systems 248-BD/CF ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with mouse Sema3a (R&D systems) diluted in growth media for 5 ...
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bioRxiv - Cancer Biology 2020Quote: Cells were incubated with GFRA2 (R&D systems, AF429) and NGFR (Cell Signalling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured together with H1152 (R&D systems) or sterile water ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with Annexin V (R&D Systems) per manufacturer’s instructions and were run immediately without fixation or were fixed in 1% PFA diluted in Annexin V binding buffer ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated with 1nM Dpp (R&D Systems) for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... cells were induced with activin-A (R&D Systems) and Matrigel (1:60 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were seeded upon fibronectin-coated (R&D Systems) Softrac hydrogels (Matrigen ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with IFN-β (R&D Systems #8499-IF-010 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with ITGB4 antibody conjugated to Alexa Fluor® 488 (5 μl/1 million cells) (R&D Systems, Minneapolis, MN, USA) and Propidium Iodide Ready Flow™ Reagent (1 drop/1 million cells ...
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bioRxiv - Bioengineering 2021Quote: ... 36 million cells were used for sorting with the Sertoli cell marker Thy-1 Cell Surface Antigen (THY1/CD90) using a PE-conjugated antibody for THY1/CD90 (R&D Systems, FAB2067P) and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then seeded onto 24 well plates (106 cells/well) with or without 10 ng/mL murine IFN-γ (R&D Systems). Sixteen hours later ...