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Citations for R&D Systems :
1951 - 2000 of 2966 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... the ears of the mice were intradermally injected with 500 ng recombinant mouse IL-23 (R&D Systems cat. 1887-ML-010) dissolved in 25 μl PBS into one ear and 25 μl PBS into the contralateral ear ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... were measured by flow cytometry 2 days after activation with or without an extra stimulation with 25 ng/mL IL-2 (R&D Systems) during 15 min.
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bioRxiv - Immunology 2021Quote: ... fifty microliters of stimulant pepmixes (JPT peptides) resuspended in RPMI+10%FBS (R10) with recombinant mouse IL-2 (rmIL-2) (R&D Systems) were added to each well ...
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Sorted cells were plated at 5000 cells per well in a 96 well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Microbiology 2022Quote: Distal ileal TNF-α and IL-6 were quantified using the mouse TNF-α enzyme-linked immunosorbent assay [ELISA] Ready-SET-Go kit (eBioscience; San Diego, CA, USA) and the Mouse IL-6 DuoSet ELISA (R&D Systems) respectively ...
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bioRxiv - Immunology 2022Quote: ... cells were washed and split into two new 48-well plate wells and cultured with IL-2 (5 ng/ml; R&D Systems), in the presence or the absence of CH-223191 (10 µM ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... Primary astrocytes were treated with 100 ng/mL IL-1β (R&D Systems, #401-ML-005, 100 µg/mL stock in PBS) and 50 ng/mL TNF (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... Adherent cell were then cultured in RPMI supplemented with 10% heat-inactivated human serum and L-Glutamine and gentamycin as above (RPMI10%HuS) and 30 ng/ml GM-CSF (Immunex) and 10 ng/ml IL-4 (R&D Systems) for 5 days50 ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by ELISA using the DuoSet ELISA Kits (DY210-05, DY201-05, and DY206-05) from R&D Systems per the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The following ELISA kits were used: TSLP mouse ELISA kit (EMTSLP; Thermo) and Mouse IL-33 Quantikine ELISA kit (M3300; R&D system). All samples were assessed in triplicate.
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β and other pro-inflammatory cytokine levels in mouse BM and plasma were measured by ELISA kits from R&D systems and Mesoscale Discovery according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated in the following primary antibodies overnight at 4L: IL-17B (1:100, Cat #1248, RRID:AB_354696, R&D Systems, Minneapolis, MN), TNF-alpha (1:100 Cat #AF-410 ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were plated at 5000 cells per well in a 96-well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... Mice were sacrificed 24h post-infection and n=7 were analysed for bacterial load and IL-6 and KC using ELISA (R&D systems). A further n=3 animals were analysed using histology ...
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bioRxiv - Immunology 2024Quote: ... the medium was enriched with recombinant murine interleukin-3 (IL-3; 5 ng/mL) and stem cell factor (SCF; 5 ng/mL) from R&D Systems, Minneapolis ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL-6 and BNP plasma levels were analyzed using the following ELISA kit: Mouse IL-6 Quantikine ELISA Kit (M6000B, R&D systems), Mouse BNP EIA (EIAM-BNP-1 ...
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bioRxiv - Cell Biology 2024Quote: Extracellular conditioned media from differentiated 3T3-L1 cells pretreated with or without palmitate and UNC0642 were quantified using mouse IL-6 Quantikine ELISA kit M6000B from R&D Systems as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatants were transferred to a new eppendorf tube and used for measuring the levels of cytokines using the Mouse TNF-alpha Quantikine ELISA kit and the Mouse IL-6 Quantikine ELISA kit (R&D systems), following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... cells were resuspended in Goat anti-Human ACE2 antibody (1:500, R&D Systems) in the flow buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with recombinant human IFNγ (R&D Systems, 285-IF-100) by adding an equal volume of the complete culture media containing the cytokine ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant FGF2 (10 ng/ml, R&D Systems, Minneapolis, Minnesota; 233-FB). After two to three weeks ...
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bioRxiv - Cell Biology 2020Quote: ... Gas1 was stained using goat anti-human Gas1 antibodies (R&D systems, Cat# AF2636). Dhh was stained using mouse anti-Dhh antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human TGF-β (7754-BH) was purchased from R&D Systems (Minneapolis, MI). It was reconstituted at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used: Human alpha-Smooth Muscle Actin (R&D Systems, MAB1420), Human/Mouse alpha-Fetoprotein/AFP (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... during 2 hours and stimulated with human recombinant VEGF165 (25ng/ml, R&D System) during 30 minutes.
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bioRxiv - Genomics 2019Quote: ... 50 ng/mL carrier-free recombinant human stem cell factor (SCF; R&D Systems), 50 ng/mL TPO (R&D Systems) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 100ng/mL human recombinant FGF4 (R&D Systems, 235-F4) in the presence of 10 μg/mL heparin sulfate and 3 μM BI-D1870 RSK inhibitor at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... PDGF-AB were determined by Human Magnetic Luminex Assays (R&D Systems, Minneapolis, MN) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... NMPM: N2B27 supplemented with 40 ng/ml recombinant human (rh) FGF2 (R&D Systems), 40 ng/ml rhFGF8 (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems) were performed according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: CCL5/RANTES was included in Human Chemokine Protein Sampler Pack from R&D systems..
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bioRxiv - Cell Biology 2020Quote: ... were incubated with 100μg/ml recombinant human Wnt10b protein (0.1mg/ml;R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Human recombinant pro-legumain (asparaginyl endopeptidase) was purchased from R&D Systems (2199-CY), activated per manufacturer instruction and active site titrated using MP-L01 inhibitor as described by Poreba et al ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Neuroscience 2019Quote: Recombinant human IDO (Accession #P14902) was obtained from R&D Systems (#6030-AO-010) and frozen at −80°C until use ...
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bioRxiv - Cancer Biology 2019Quote: ... Recombinant human TGFβ-1 (cat# 240B002) was purchased from R&D systems (Minneapolis, MN). Alexa 488-conjugated phalloidin (cat# A12379 ...
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bioRxiv - Neuroscience 2020Quote: ... the following antibodies were used: anti-human Nestin (R&D Systems, MAB1259, 1:2,400), goat anti-TrkB (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... then labelled with and without anti-human LILRB2 (5 µg/mL; R&D Systems). The cells were washed with PBS (0.5% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... and Human chitinase 3-like 1 (YKL-40) were purchased from R&D Systems. Bovine serum albumin was purchased from Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: Proteome Profiler Antibody Array Kits for human angiogenesis factors (R&D systems, MN, USA) were used for reverse western blot ...
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bioRxiv - Biochemistry 2020Quote: The mouse and human anti-CHL1 antibodies (AF2147 and MAB2126; R&D systems, MN) were partially reduced and bound to HRP using a peroxidase labeling kit SH (Dojindo ...