Labshake search
Citations for R&D Systems :
2201 - 2250 of 2966 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... The release of cytokines IL-1β and TNF-α into supernatants was determined with ELISA kits (R&D Systems, DY401-05 and DY410-05) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: PBMC (2×106 per well) were stimulated in 24-well plates with peptide (individual/pool) plus recombinant IL-2 (R&D Systems Europe Ltd.) at a final concentration of 5μg/mL and 20IU/mL ...
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bioRxiv - Pathology 2019Quote: ... Islets from each donor were treated with or without 24 hours of a cytokine cocktail of IL-1β (50 U/ml) and INF-γ (1000 U/ml) (R&D Systems, USA). Total RNA was recovered using RNeasy mini kits (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
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bioRxiv - Bioengineering 2020Quote: ... medium was removed and the scaffolds designated for study in normal growth media were placed in wells with fresh normal growth medium and the mineralized collagen-AM scaffolds designated for study in inflammatory conditions were placed in wells with inflammatory medium (1 ng/ml of IL-1β recombinant porcine protein (R&D Systems, Minneapolis, MN) added to normal growth media) ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 were assayed using a Luminex mouse pre-mixed Multi-Analyte kit according to standard instructions (LXSARM-06, R&D System, Minnesota, USA).
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bioRxiv - Immunology 2024Quote: ... Lymphocytes from SP and draining LNs were cultured in the presence of IRBP-p (10 μg/ml) and IL-23 (R&D systems, Minneapolis, MN) (10 ng/ml ...
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bioRxiv - Microbiology 2023Quote: IL-6 ELISAs were performed on supernatant from mouse lung homogenates using the Mouse IL-6 Duoset ELISA kit from R&D Systems (catalog # DY406) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Bronchoalveolar lavage fluid or conditioned culture media were collected for measurement of IL-6 with a Duoset ELISA kit (R&D Systems, DY406-05). Capture antibody was coated overnight in a 96 well plate at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: PBMC (2×106 per well) were stimulated in 24-well plates with peptide (individual/pool) plus recombinant IL-2 (R&D Systems Europe Ltd.) at a final concentration of 5µg/mL and 20IU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cytokines level were measured using either the Proteome Profiler Human XL Cytokine array (R&D Systems) or Quantikine ELISA kits for dedicated cytokines (R&D Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... human CXCL1/GROα duo-set or CCL5 duo-set or IGFBP3 duo-set (R&D Systems) were used to detect the concentrations of CXCL1 or CCL5 or IGFBP3 in the plasma following manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... human CXCL1/GROα duo-set or CCL5 duo-set or IGFBP3 duo-set (R&D Systems) were used to detect the concentrations of CXCL1 or CCL5 or IGFBP3 in the CM following manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Biochemistry 2022Quote: ... or 1:1000 anti-Human/Mouse/Rat PRL-3 Antibody (R&D Systems, MAB3219, Lot. WXH0419091). Following three washes with 0.1% TBST ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... Progranulin protein levels were measured using a human-specific ELISA (Quantikine and DuoSet; R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated with recombinant human CTLA-4-Fc (7268-CT, R&D Systems, Minneapolis, MN, USA) at 1 μg/mL overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... the following primary antibodies were used: mouse anti-human IFN-γ (R&D system, 1:1000), mouse anti-human ICAM1 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... were selected and transferred to 1mg/ml recombinant human Noggin protein (6057-NG, R&D Systems) in PBS and incubated for 30 minutes to 1 hour at ambient temperature before being implanted in embryos ...
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bioRxiv - Genomics 2019Quote: ... and 10 ng/ml recombinant human transforming growth factor beta 3 (rhTGF-b3) (R&D Systems). Pellets were formed at the end of passage 6 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were preincubated for 10min with 30µg rat anti-human neuropilin1 Fc chimera (R&D System) or 20µg goat anti-human neuropilin2 Fc chimera (R&D System ...
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bioRxiv - Biochemistry 2020Quote: Recombinant proteins of human ACE2 extracellular enzymatic domain were purchased from R&D systems(933-ZN) and Sinobiological ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Cyto C Immunoassay 96-well plates followed by ELISA detection (R&D Systems, Minneapolis, MN). The fractional release of mitochondrial cytochrome c was calculated for each condition as [mean intensitysupernatant/(mean intensitymitochondria + mean intensitysupernatant)] ...
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bioRxiv - Cancer Biology 2020Quote: ... Phosphorylation array was performed by using the human phosphor-kinase array kit (R&D systems, USA).
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/mL recombinant human insulin-like growth factor 1 (IGF-1) (R&D Systems, USA), and 10 μM SB431542 (Tocris ...
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bioRxiv - Cell Biology 2021Quote: TSP1 levels were measured using the Human Thrombospondin-1 DuoSet ELISA kit (R&D systems; DY3074) in the supernatant surrounding treated platelets ...
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bioRxiv - Microbiology 2021Quote: ... and IP10 were determined using a Non-Human Primate Customized Multiplex (R&D Systems, Minneapolis, USA) following manufacturer’s instruction by using a Magpix (Luminex ...
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bioRxiv - Systems Biology 2021Quote: ... VEGF concentration was measured using a Human VEGF Quantikine ELISA Kit (R&D Systems, Minneapolis, MN). The cell number in each well was determined by trypsinization and counting of cells with a hemocytometer on days 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Human alpha-Smooth Muscle Actin APC-conjugated Antibody (αSMA-APC) (R&D systems IC1420A, Clone #1A4) was added for 30 min before cells were washed in PW-buffer.
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bioRxiv - Bioengineering 2021Quote: ... and recombinant human ST6Gal sialyltransferase 1/ST6GAL1 (aa 44-406) from R&D Systems (Bio-techne). FabRICATOR enzyme was purchased from Genovis.
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bioRxiv - Genomics 2022Quote: ... cells were incubated with indicated concentrations (Figure 1B) of human TGF-β1 protein (R&D systems) in the complete culture medium ...
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bioRxiv - Bioengineering 2022Quote: ... Human α-Smooth Muscle Actin (SMA) Alexa Fluor® 647-conjugated antibody (R&D Systems-IC1420R) diluted 1:200 was introduced for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The recombinant cytokines that were used were Recombinant Human GM-CSF Protein (R&D Systems, 215GM), Recombinant Human CCL20/MIP-3α Protein (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: Medium from cells was harvested and used in the Human Cytokine Array Kit (R&D Systems), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... When testing the effects of human recombinant FN1 (rFN1) (R&D Systems Catalog number: 4305-FNB), rFN1 was prepared in serum free DMEM at the concentration of either 10 ng/mL or 20 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: Same amount (1 μM) of purchased Human IMPDH2 recombinant protein (8349-DH-050, R&D systems) was incubated in 50 mM HEPES buffer (pH 8.5 ...
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bioRxiv - Microbiology 2021Quote: MBL levels in patient plasma were determined using Human MBL Quantikine ELISA Kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the following primary antibodies were used: anti-human PTN (1:200; R&D systems, AF252-PB), anti-PanCK ...
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bioRxiv - Immunology 2019Quote: ... following relevant treatment with or without 200 U/ml of recombinant human TNF (R&D Systems). Protein concentration was measured using the Bradford assay (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human ubiquitin-activating enzyme E1 (UBE1) was purchased from R&D systems (Cat. # E-305). Recombinant human UbcH5a was purchased from R&D systems (Cat ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant human SUMO-1 and SUMO-2 were purchased from R&D systems (Cat. # K-700). Recombinant human SUMO activating enzyme E1 (SAE1/UBA2 ...
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bioRxiv - Cancer Biology 2021Quote: ... IGF2 concentration was determined by using the Human IGF2 Quantikine ELISA Kit (R&D Systems #DG200) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... The anti-human ADAM17 mAbs 111633 and 111623 were purchased from R&D Systems (Minneapolis, MN). The mouse anti-human ADAM17 mAb M220 (generously provided by Dr ...
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bioRxiv - Genomics 2021Quote: ... Human spinal cord 4i data was generated over six cycles using CD34 (R&D Systems, AF7227), LMNB1 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Secreted IFNβ1 was detected with human IFN-beta DuoSet ELISA kit (R&D systems, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The human active recombinant Caspase-3 (#707-C3) was purchased from R&D Systems (Minneapolis, USA). Purified Human active Cathepsin-B (#BML-SE198) ...