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1951 - 2000 of 10000+ citations since 2019

• Lymphatic vessel development in human embryos

  Shoichiro Yamaguchi, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Flk1 (AF357, R&D Systems, 1:150, RRID:AB_355320), Isl1 (PA5-27789 ...
• DNL343 is an investigational CNS penetrant eIF2B activator that prevents and reverses the effects of neurodegeneration caused by the Integrated Stress Response

  Ernie Yulyaningsih, et al., bioRxiv - Neuroscience 2023

  Quote: ELISA kits were used to determine the levels of GDF15 (mouse/rat GDF15 Quantikine ELISA Kit, R&D Systems, catalog # MGD150), TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit ...
• DNL343 is an investigational CNS penetrant eIF2B activator that prevents and reverses the effects of neurodegeneration caused by the Integrated Stress Response

  Ernie Yulyaningsih, et al., bioRxiv - Neuroscience 2023

  Quote: ... and MCP-1 (Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit, R&D Systems, catalog # MJE00B) in the brain ...
• DNL343 is an investigational CNS penetrant eIF2B activator that prevents and reverses the effects of neurodegeneration caused by the Integrated Stress Response

  Ernie Yulyaningsih, et al., bioRxiv - Neuroscience 2023

  Quote: ... TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit, R&D Systems, catalog # MTM100) and MCP-1 (Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit ...
• Bulky glycocalyx drives cancer invasiveness by modulating substrate-specific adhesion

  Amlan Barai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or 0.2ug/mL E-selectin (Cat# 724-ES, R&D Systems) overnight at 4°C.
• Competitive specific anchorage of molecules onto surfaces: quantitative control of grafting densities and contamination by free anchors

  Oksana Kirichuk, et al., bioRxiv - Biophysics 2023

  Quote: A recombinant P-selectin-Fc fusion protein (R&D Systems # 137-PS; ∼300 kDa) was purchased from Bio-Techne (Abingdon ...
• Lymphatic vessel development in human embryos

  Shoichiro Yamaguchi, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Prox1 (AF2727, R&D Systems, 1:150, RRID:AB_2170716), LYVE1 (ab14917 ...
• Lymphatic vessel development in human embryos

  Shoichiro Yamaguchi, et al., bioRxiv - Developmental Biology 2023

  Quote: ... VEGFR-3 (AF349, R&D Systems, 1:100, RRID:AB_355314), Coup-TF2 (EPR18443 ...
• Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration

  Simon Perrin, et al., bioRxiv - Cell Biology 2023

  Quote: ... Goat polyclonal to mouse Periostin (AF2955, R&D Systems), Goat polyclonal to mouse PECAM1 (AF3628 ...
• Comparative Interactome Analysis of α-arrestin Families in Human and Drosophila

  Kyung-Tae Lee, et al., bioRxiv - Cell Biology 2023

  Quote: ... after which the medium was changed to α-MEM containing 20 ng/ml M-CSF and 20 ng/ml RANKL (462-TEC; R&D Systems, Minneapolis, MN, USA). The differentiation medium was changed every 24 hr during the 5-day differentiation period.
• ISG15/USP18/STAT2 is a molecular hub regulating autocrine IFN I-mediated control of Dengue and Zika virus replication

  Constanza Eleonora Espada, et al., bioRxiv - Immunology 2023

  Quote: ... mouse mAb to ISG15 (R&D System, MAB4845, dilution 1:1000), home-made mouse mAb anti-GFP [67 ...
• Effect of gedunin on glioblastoma proliferation and invasiveness: In vitro and in vivo approaches

  Thadeu Estevam Moreira Maramaldo Costa, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: Levels of vascular endothelial growth factor (VEGF) were evaluated by sandwich enzyme-linked immunosorbent assay (ELISA) in GL261 cell supernatants using matched antibody pairs from R&D Systems (USA), according to the manufacturer’s instructions ...
• Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

  Kira Cozzolino, et al., bioRxiv - Biochemistry 2023

  Quote: ... 1x GlutaminePlus (R&D Systems, R90210), and 1x Penicillin-Streptomycin (Gibco ...
• Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome

  Kira Cozzolino, et al., bioRxiv - Biochemistry 2023

  Quote: ... and 250 μg of protein from each condition was incubated with a membrane array from the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare) ...
• Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity

  Jack Polmear, et al., bioRxiv - Immunology 2023

  Quote: ... 100 ng/mL APRIL (R&D Systems) diluted in supernatant from human tonsillar fibroblasts ...
• Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity

  Jack Polmear, et al., bioRxiv - Immunology 2023

  Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
• Small transcriptional differences among cell clones lead to distinct NF-κB dynamics

  Cise Kizilirmak, et al., bioRxiv - Systems Biology 2023

  Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
• Synergistic induction of blood-brain barrier properties

  Gergő Porkoláb, et al., bioRxiv - Bioengineering 2023

  Quote: ... goat anti-CD13 (R&D Systems, cat# AF2335, AB_2227288, 1:200), mouse anti-vimentin (Invitrogen ...
• Trabid patient mutations impede the axonal trafficking of adenomatous polyposis coli to disrupt neurite growth

  Daniel Frank, et al., bioRxiv - Cell Biology 2023

  Quote: ... or K33-linked Tetra-Ub chains (R&D Systems) in DUB reaction buffer (50 mM NaCl ...
• The actin binding protein profilin 1 is critical for mitochondria function

  Tracy-Ann Read, et al., bioRxiv - Cell Biology 2023

  Quote: ... supplemented with 8% fetal calf serum (R&D Systems) and 1% penicillin-streptomycin (Corning) ...
• Murine GMP- and MDP-derived classical monocytes have distinct functions and fates

  Sébastien Trzebanski, et al., bioRxiv - Immunology 2023

  Quote: ... goat polyclonal anti-CD206 (1:250, R&D Systems, #AF2535), rat monoclonal anti-MHC class II (I-A/I-E ...
• The nuclei of human adult stem cells can move within the cell and generate cellular protrusions to contact other cells

  Carlos Bueno, et al., bioRxiv - Cell Biology 2023

  Quote: ... supplemented with N2-supplement (R&D systems), 0.6% glucose (Sigma-Aldrich) ...
• The actin binding protein profilin 1 is critical for mitochondria function

  Tracy-Ann Read, et al., bioRxiv - Cell Biology 2023

  Quote: ... supplemented with 8% fetal calf serum (R&D Systems) and 1% penicillin-streptomycin (Corning) ...
• The juxtamembrane linker of synaptotagmin 1 regulates Ca²⁺ binding via liquid-liquid phase separation

  Nikunj Mehta, et al., bioRxiv - Neuroscience 2023

  Quote: ... supplemented with 10% fetal bovine serum (FBS; R&D Systems, S11550H) and penicillin-streptomycin (Thermo Fisher Scientific ...
• Evidence suggesting creatine as a new central neurotransmitter: presence in synaptic vesicles, release upon stimulation, effects on cortical neurons and uptake into synaptosomes and synaptic vesicles

  Xiling Bian, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse anti Creatine Kinase B (dilution 1:5000, cat no MAB9076, R&D Systems), rabbit anti HA (dilution 1:2000 ...
• Time-, tissue- and treatment-associated heterogeneity in tumour-residing migratory DCs

  Colin YC Lee, et al., bioRxiv - Immunology 2023

  Quote: ... 1% normal rat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a humid chamber with the appropriate antibodies ...
• Rab7 dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis

  Preksha Gaur, et al., bioRxiv - Cell Biology 2023

  Quote: ... The samples were quantified by BCA assay and further equal protein was assayed by enzyme-linked immunosorbent assay (ELISA) for TNF-a using the manufacturer’s protocol (R&D systems, USA).
• Expression of the transcription factor Isl1 in dopaminergic neurons of the mouse forebrain

  Adriana C. Camarano, et al., bioRxiv - Developmental Biology 2023

  Quote: ... goat anti-Isl1 (1:1000; R&D Systems AF1837); mouse anti-Nkx2.2 (1:1000 ...
• Nerve Growth Factor Receptor Limits Inflammation to Promote Remodeling and Repair of Osteoarthritic Joints

  Lan Zhao, Yumei Lai, Hongli Jiao, Jian Huang, bioRxiv - Cell Biology 2023

  Quote: ... 1/200 anti-βIII-tubulin antibody (R&D Systems, catalog # MAB1195) overnight at 4°C and incubated with secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... after which the organoids were cultured for 13 days in E6 supplemented with 50 ng/mL bone morphogenetic protein-7 (BMP7, R&D systems) and 10 ng/mL human epidermal growth factor (hEGF ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... The primary antibodies used in this study were sheep anti-nephrin (#AF4269-SP, R&D systems, Abingdon, United Kingdom) diluted 1:300 ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... after which the cells were exposed for 3 days to 200 ng/ml FGF-9 (R&D systems) and 1μg/mL heparin (Sigma Aldrich ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... medium supplemented with 6 μM CHIR99021 (R&D systems). Ureteric bud progenitor cells were generated by exposing the cells to the indicated CHIR99021 concentration for 4 days in E6 medium ...
• A novel KCNJ16 kidney organoid model recapitulates the disease phenotype and shows restoration of lipid accumulation upon treatment with statins

  E. Sendino Garví, et al., bioRxiv - Cell Biology 2023

  Quote: ... sheep anti-fibronectin (#AF1918, R&D systems) diluted 1:400 ...
• In vivo PSC differentiation as a platform to identify factors for improving the engraftability of cultured muscle stem cells

  Ning Xie, et al., bioRxiv - Cell Biology 2023

  Quote: ... 10 ng/mL basic FGF (R&D Systems #233-FB/CF, Minneapolis, MN), 1% P/S ...
• In-utero exposure to estrogen-mimicking bisphenols alters bone mineralization in the offspring

  Saikanth Varma, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Recombinant human Noggin protein-carrier free (#6057-NG/CF) was obtained from R&D systems.
• First cleavage is a manifestation of the geometry of the unfertilized oocyte: implications for monozygotic twinning in mice

  Thomas Nolte, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CDX2-88), anti-Nanog rabbit IgG (Cosmo Bio, Tokyo, Japan, cat. no. REC-RCAB0002P-F) and anti-Sox17 goat IgG (R&D Systems, cat no. AF1924) in dilutions of 1:200 ...
• Systems genetics uncover new loci containing functional gene candidates in Mycobacterium tuberculosis-infected Diversity Outbred mice

  DM Gatti, et al., bioRxiv - Genetics 2023

  Quote: ... and vascular endothelial growth factor (VEGF) by sandwich ELISA using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
• Comparison of gastric inflammation and metaplasia induced by Helicobacter pylori or Helicobacter felis colonization in mice

  Sara R. Druffner, et al., bioRxiv - Immunology 2023

  Quote: ... containing 5% fetal bovine serum (R&D Systems) and 10 mg/mL vancomycin and grown overnight at 37°C under microaerophilic conditions with agitation ...
• DONOR VARIABILITY IN HUMAN MESENCHYMAL STEM CELL OSTEOGENIC RESPONSE AS A FUNCTION OF PASSAGE CONDITIONS AND DONOR SEX

  Vasiliki Kolliopoulos, et al., bioRxiv - Bioengineering 2023

  Quote: An enzyme-linked immunosorbent assay (ELISA) (R&D Systems, Minneapolis, MN) was used to quantify the amount of osteoprotegerin (OPG ...
• Structural mechanism of angiogenin activation by the ribosome

  Anna B. Loveland, et al., bioRxiv - Biochemistry 2023

  Quote: Recombinant human angiogenin (catalog number 265-AN/CF) was purchased from R&D Systems, a Bio-Techne Company (Minneapolis ...
• Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches

  John M. Viola, et al., bioRxiv - Bioengineering 2023

  Quote: ... 1x Penn/Strep and 10 ng ml-1 FGF9 (R&D Systems, 273-F9-025). On day 9 (metanephric mesenchyme stage) ...
• Activin A targets extrasynaptic NMDA receptors to improve neuronal and behavioral deficits in a mouse model of Huntington disease

  Wissam B Nassrallah, et al., bioRxiv - Neuroscience 2023

  Quote: ... Activin A levels were measured using an ELISA kit (Human/Mouse/Rat Activin A Quantikine ELISA Kit, R&D Systems, DAC00B).
• Substrate identification and specificity profiling of deubiquitylases against endogenously-generated ubiquitin-protein conjugates

  Valentina Rossio, et al., bioRxiv - Biochemistry 2023

  Quote: ... 50 μM of human recombinant untagged ubiquitin (R&D SYSTEMS, U-100H) and specific human recombinant DUBs were added at the same concentration as for the proteomic experiments (800 nM) ...
• Insulin receptor turnover in fasting is dependent on β-dystroglycan deglycosylation

  Sunu Joseph, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-cathepsin D (Cat# MAB1029) from R&D systems, and anti-EEA (Cat# 1610456 ...
• Substrate identification and specificity profiling of deubiquitylases against endogenously-generated ubiquitin-protein conjugates

  Valentina Rossio, et al., bioRxiv - Biochemistry 2023

  Quote: ... Single human recombinant DUBs (800 nM) were added together with 50 μM human recombinant HA-tagged ubiquitin (R&D SYSTEMS, U110) to each reaction ...
• Type I interferons sensitise HIV-1-reactivating T-cells for NK cell-mediated elimination despite HDACi-imposed dysregulation of innate immunity

  Julia Prigann, et al., bioRxiv - Microbiology 2023

  Quote: ... The following primary antibodies were used: anti-90K/LGALS3BP (#AF2226: R&D Systems), anti-BST2/Tetherin (#390719 ...
• Evolution of E. coli in a mouse model of inflammatory bowel disease leads to a disease-specific bacterial genotype and trade-offs with clinical relevance

  Rahul Unni, et al., bioRxiv - Evolutionary Biology 2023

  Quote: Lipocalin-2 concentration in the supernatants was measured using the commercial kit Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) for mouse Lipocalin-2 (DY1857) ...
• An engineered biosensor enables dynamic aspartate measurements in living cells

  Kristian Davidsen, et al., bioRxiv - Biochemistry 2023

  Quote: Cell lines were acquired from ATCC (HEK293T, H1299, HT1080) and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
• Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment

  Gabriela Traczyk, et al., bioRxiv - Cell Biology 2023

  Quote: ... samples were subjected to immunoblotting with sheep anti-DGKε antibody (R&D Systems) (Supplemental Table S2).