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1851 - 1900 of 10000+ citations since 2019

• TLR2 Regulates Hair Follicle Cycle and Regeneration via BMP Signaling

  Luyang Xiong, et al., bioRxiv - Immunology 2023

  Quote: ... P-cadherin (R&D systems, AF761-SP), CEP (Pacific Immunology) ...
• Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

  Yin Luo, et al., bioRxiv - Cell Biology 2023

  Quote: ... Bound TRAIL was stained with goat anti-mTRAIL (1ug/ml, AF1121, R&D systems) or goat anti-hTRAIL (1ug/ml ...
• Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

  Yin Luo, et al., bioRxiv - Cell Biology 2023

  Quote: Apoptosis of tumor cells was determined with an Annexin-FITC staining kit (R&D Systems) as per the manufacturer’s instructions and analyzed by flow cytometry ...
• Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

  Yin Luo, et al., bioRxiv - Cell Biology 2023

  Quote: ... or goat anti-hTRAIL (1ug/ml, AF375, R&D systems) for 1 h at 4 °C ...
• Evidence suggesting creatine as a new central neurotransmitter: presence in synaptic vesicles, release upon stimulation, effects on cortical neurons and uptake into synaptosomes and synaptic vesicles

  Xiling Bian, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-CathepsinB (dilution 1:2000, AF965, R&D Systems), rabbit anti-GAPDH (dilution 1:1000 ...
• MAPK/ERK Activation in Macrophages Promotes Leishmania Internalization and Pathogenesis

  Umaru Barrie, et al., bioRxiv - Microbiology 2023

  Quote: ... and MIP-1β (CCL4) were profiled using the custom multiplex Luminex Platform (R&D Systems, Inc, Minneapolis, MN) through the UT Southwestern Microarray Core as described previously [44 ...
• The juxtamembrane linker of synaptotagmin 1 regulates Ca²⁺ binding via liquid-liquid phase separation

  Nikunj Mehta, et al., bioRxiv - Neuroscience 2023

  Quote: ... supplemented with 10% fetal bovine serum (FBS; R&D Systems, S11550H) and penicillin-streptomycin (Thermo Fisher Scientific ...
• Evidence suggesting creatine as a new central neurotransmitter: presence in synaptic vesicles, release upon stimulation, effects on cortical neurons and uptake into synaptosomes and synaptic vesicles

  Xiling Bian, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse anti Creatine Kinase B (dilution 1:5000, cat no MAB9076, R&D Systems), rabbit anti HA (dilution 1:2000 ...
• Evolution of E. coli in a mouse model of inflammatory bowel disease leads to a disease-specific bacterial genotype and trade-offs with clinical relevance

  Rahul Unni, et al., bioRxiv - Evolutionary Biology 2023

  Quote: Lipocalin-2 concentration in the supernatants was measured using the commercial kit Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) for mouse Lipocalin-2 (DY1857) ...
• An engineered biosensor enables dynamic aspartate measurements in living cells

  Kristian Davidsen, et al., bioRxiv - Biochemistry 2023

  Quote: Cell lines were acquired from ATCC (HEK293T, H1299, HT1080) and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
• Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment

  Gabriela Traczyk, et al., bioRxiv - Cell Biology 2023

  Quote: ... samples were subjected to immunoblotting with sheep anti-DGKε antibody (R&D Systems) (Supplemental Table S2).
• Time-, tissue- and treatment-associated heterogeneity in tumour-residing migratory DCs

  Colin YC Lee, et al., bioRxiv - Immunology 2023

  Quote: ... 1% normal rat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a humid chamber with the appropriate antibodies ...
• Rab7 dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis

  Preksha Gaur, et al., bioRxiv - Cell Biology 2023

  Quote: ... The samples were quantified by BCA assay and further equal protein was assayed by enzyme-linked immunosorbent assay (ELISA) for TNF-a using the manufacturer’s protocol (R&D systems, USA).
• Pathological mutations reveal the key role of the cytosolic iRhom2 N-terminus for phosphorylation-independent 14-3-3 interaction and ADAM17 binding, stability, and activity

  Katharina Bläsius, et al., bioRxiv - Molecular Biology 2023

  Quote: ... were as follows: αADAM17 (1:100; R&D Systems; MAB 9301), αHA (1:500 ...
• Type I interferons sensitise HIV-1-reactivating T-cells for NK cell-mediated elimination despite HDACi-imposed dysregulation of innate immunity

  Julia Prigann, et al., bioRxiv - Microbiology 2023

  Quote: ... The following primary antibodies were used: anti-90K/LGALS3BP (#AF2226: R&D Systems), anti-BST2/Tetherin (#390719 ...
• Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains

  Kaitlin S. Radmall, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 5 mM ATP) with 0.1 μM recombinant yeast E1 (R&D Systems), ...
• Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment

  Gabriela Traczyk, et al., bioRxiv - Cell Biology 2023

  Quote: ... The following antibodies were used: sheep anti-DGKε (R&D Systems, cat. No. AF7069), mouse anti-Myc (ThermoFisher ...
• Structure-function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains

  Kaitlin S. Radmall, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 50 μM recombinant yeast ubiquitin (R&D Systems), 5 μM Rad6 or Rad6-150 ...
• Ym1 protein crystals promote type 2 immunity

  Ines Heyndrickx, et al., bioRxiv - Immunology 2023

  Quote: ... the pellets obtained after centrifugation of the BAL fluid were pooled and stained for 30 min at 4°C with unconjugated polyclonal goat anti-mouse Ym1/2 (AF2446, R&D Systems; 1:100 diluted in PBS). After washing ...
• Ym1 protein crystals promote type 2 immunity

  Ines Heyndrickx, et al., bioRxiv - Immunology 2023

  Quote: ... CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems). Serum Ym1-specific immunoglobulin M (IgM ...
• Proliferating CLL cells express high levels of CXCR4 and CD5

  Daniel Friedman, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 0.5% BSA plus 0.1/1 μg/mL CCL21 (R&D systems) was added to basolateral chambers ...
• Proliferating CLL cells express high levels of CXCR4 and CD5

  Daniel Friedman, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Transwell filters were coated overnight with 2.5μg/ml ICAM-1 (R&D systems) in PBS and blocked with 2% BSA/PBS for 2hrs ...
• Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2

  Aswini Panigrahi, et al., bioRxiv - Cancer Biology 2023

  Quote: ... LG3BP (R&D Systems) at a 2-fold dilution series (concentration range of 2-16 μg/ml ...
• Single-molecule FRET and molecular dynamics simulations reveal early activation steps of MET receptor by Listeria monocytogenes

  Yunqing Li, et al., bioRxiv - Biophysics 2023

  Quote: ... The primary antibody (goat@MET, #AF276, R&D Systems, USA) was diluted in BB to a final concentration of 2 µg/mL and incubated for 2 h at room temperature with gentle shaking ...
• Preclinical studies with ground germinated barley (GGB) for oral enzyme replacement therapy (Oral-ERT) in Pompe disease knockout mice

  Frank Martiniuk, et al., bioRxiv - Genetics 2023

  Quote: We compared a lysate of seeds to a rhGAA (R&D Systems) and mature placental human GAA for specific activity using 4-MU-Glc at pH 4.0 ...
• Preclinical studies with ground germinated barley (GGB) for oral enzyme replacement therapy (Oral-ERT) in Pompe disease knockout mice

  Frank Martiniuk, et al., bioRxiv - Genetics 2023

  Quote: ... Mock treated GAA and normal myoblast cells were controls plus cells treated with equivalent amounts of a rhGAA (R&D Systems #8329-GH-025). Simultaneously ...
• Transcriptional and Cellular Response of hiPSC-derived Microglia-Neural Progenitor Co-Cultures Exposed to IL-6

  Amalie C. M. Couch, et al., bioRxiv - Neuroscience 2023

  Quote: ... Cytokine profiling was carried out following the manufactures instructions using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems; ARY022B). The kit contained membranes with dots of 105 different immobilised cytokine antibodies dotted with two technical replicates each (Supplementary Figure 5) ...
• PARP inhibitors enhance reovirus-mediated cell killing through the death-inducing signaling complex (DISC) with an associated NF-κB-regulated immune response

  Joan Kyula-Currie, et al., bioRxiv - Cancer Biology 2023

  Quote: ... caspase-3 and PARP cleavage were determined by Western Blot analysis while levels of PAR in the tumor lysates were determined by ELISA using the HT PARP in vivo PD Assay II Kit [4520-096-K] from R&D Systems. The remaining mice were measured twice weekly in three dimensions using Vernier calipers and the volume estimated using the formula (width x length x depth x 0.524mm3) ...
• RNA 5ʹ terminal nucleotide determines the strength of the RIG-I/IFN signaling pathway

  Magdalena Wolczyk, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Recombinant human IFN-β (R&D Systems #8499-IF-010) treatment was used to induce RIG-I expression and to correctly select clones with the lowest RIG-I level ...
• SIK2: A novel negative feedback regulator of FGF signaling

  Gamze Kuser-Abali, et al., bioRxiv - Molecular Biology 2023

  Quote: ... constitutively active ERK2 (R&D Systems) and immunoprecipitated GFP-SIK2 (KI ...
• SIK2: A novel negative feedback regulator of FGF signaling

  Gamze Kuser-Abali, et al., bioRxiv - Molecular Biology 2023

  Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
• Overexpression of the WWE domain of RNF146 modulates poly-(ADP)-ribose dynamics at sites of DNA damage

  Rasha Q. Al-Rahahleh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... PAR (R&D systems; 100nM) was added to each well ...
• RNA 5ʹ terminal nucleotide determines the strength of the RIG-I/IFN signaling pathway

  Magdalena Wolczyk, et al., bioRxiv - Molecular Biology 2023

  Quote: ... A standard curve was generated in parallel by serial dilutions of recombinant IFN-β standard in DMEM (R&D Systems #8499-IF-010 and #8234-MB-010). After overnight incubation (24 h) ...
• Porphyromonas gingivalis fuels colorectal cancer through CHI3L1-mediated iNKT cell-driven immune evasion

  Angélica Díaz-Basabe, et al., bioRxiv - Immunology 2023

  Quote: ... were pre-incubated with rhCHI3L1 (R&D Systems) or Pg-primed iNKT cells for 24 hours at 37°C before performing the assay.
• Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood Onset

  L. Pérez-Revuelta, et al., bioRxiv - Neuroscience 2023

  Quote: ... IGF-1 and VEGF-B in tissue homogenates and plasma samples were determined by double-antibody sandwich ELISA according to the manufacturer instructions (IGF-1, MG100 and VEGF-A, MMV00, R&D Systems, USA ...
• The Primary Cilium and its Hedgehog Signaling in Nociceptors Contribute to Inflammatory and Neuropathic Pain

  Lindsey A. Fitzsimons, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-TrkA (tropomyosin receptor kinase A; R&D Systems, Minneapolis, MN) 1:300 ...
• Combinatorial microRNA activity is essential for the transition of pluripotent cells from proliferation into dormancy

  Dhanur P. Iyer, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 25 ng/µl FGF4 (R&D Systems, 235-F4-025) and 1 µg/ml Heparin (Sigma ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... HA-SUMO1 Vinyl Sulfone (VS) and HA-Ubiquitin VS (R&D Systems) were added to all reactions containing purified Strep-HA-TOPORS proteins in order to inhibit deubiquitinase and SUMO protease activities co-purifying with Strep-HA-TOPORS.
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... UBE2I (R&D Systems, 0.5 μM), SUMO2 (R&D Systems ...
• Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end joining reactions independently of 53BP1-governed repair pathway choice

  Ashleigh King, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and TGFβ1 (2.5ng/μl; R&D Systems). Cell-surface IgA expression was determined by flow cytometric staining with anti-mouse IgA-PE antibody (Cambridge Bioscience ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... SUMO2 (R&D Systems, 2 μM), poly-SUMO22-8 chains (R&D Systems ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... the following final concentrations were used: FLAG Ubiquitin (R&D Systems, 20 μM), ATP (Sigma-Aldrich ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... UBE2K (R&D Systems, 0.28 μM), SAE1-UBA2 (R&D Systems ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... SAE1-UBA2 (R&D Systems, 100 nM), UBE2I (R&D Systems ...
• Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation

  Julio C.Y. Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... poly-SUMO22-8 chains (R&D Systems, 0.3 μg per reaction), RNF4 (47 nM) ...
• Shieldin and CST co-orchestrate DNA polymerase-dependent tailed-end joining reactions independently of 53BP1-governed repair pathway choice

  Ashleigh King, et al., bioRxiv - Molecular Biology 2023

  Quote: ... mouse IL-4 (5 ng/μl; R&D Systems) and TGFβ1 (2.5ng/μl ...
• Precise Therapeutic Targeting of Distinct NRXN1^+/- Mutations

  Michael B. Fernando, et al., bioRxiv - Neuroscience 2023

  Quote: ... organoid maturation media was supplemented with 20 ng/ml of EGF (R&D Systems, #236-EG) and 20 ng/ml FGF2 (R&D Systems ...
• Precise Therapeutic Targeting of Distinct NRXN1^+/- Mutations

  Michael B. Fernando, et al., bioRxiv - Neuroscience 2023

  Quote: ... and 20 ng/ml FGF2 (R&D Systems, #233-FB-01M). Media was changed every day from d6 – d15 ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... following 5 min of stimulation with 10 ng/ml PDGF-BB (R&D Systems, Minneapolis, MN, USA) in the case of the PDGFRαV1/βV2 heterodimer cell line ...
• Precise Therapeutic Targeting of Distinct NRXN1^+/- Mutations

  Michael B. Fernando, et al., bioRxiv - Neuroscience 2023

  Quote: ... Ara-C and 2% low-hemoglobin FBS (R&D systems #S11510). iGLUT neurons were distributed among wells (500-750k cells per 24wp or 0.75-1.5E6 cell per 12wp ...