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Citations for R&D Systems :
1901 - 1950 of 2336 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Activin A levels were measured using an ELISA kit (Human/Mouse/Rat Activin A Quantikine ELISA Kit, R&D Systems, DAC00B).
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bioRxiv - Bioengineering 2024Quote: Purified mouse monoclonal IgG anti-human interferon-γ capture antibody (Cat. #MAB2852, lot #FIO1022021, R&D Systems, Minneapolis, Minnesota, United States), biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... the platelets were washed twice in PBS and opsonized with anti-human HLA class I (R&D Systems, 2.5 µg/mL), IgG control (R&D Systems ...
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bioRxiv - Developmental Biology 2023Quote: ... NSPC culture in adherent and non-adherent proliferating conditions using media supplemented with human recombinant EGF and FGF2 (R&D Systems) was performed according to published protocols (Soldati et al ...
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bioRxiv - Microbiology 2023Quote: Supernatants harvested from infected cells were assayed using enzyme-linked immunosorbent assay (ELISA) kits for human IL-18 (R&D Systems) and IL-1β (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Immunology 2023Quote: ... K562 cells were stimulated in complete RPMI media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems), 20 ng/mL human IFN-β (84-99I-F010 ...
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bioRxiv - Immunology 2023Quote: ... HUT78 cells were stimulated in complete IMDM media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems). All treatments were 24 hours.
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ levels were measured in NK cell media samples using the human IFN-γ DuoSet ELISA kit (R&D Systems). IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems) (53 ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: The concentration of some of the plasma markers was measured by ELISA using Human sCD14 DuoSet ELISA (R&D Systems, DY383) and Human FABP2/I-FABP DuoSet ELISA (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... and S100A8/A9 concentrations were determined using the human S100A8/A9 heterodimer ELISA kits (R&D Systems, DS8900, and DY8226-05) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were cultured for 8 days and stimulated with 2.5 ng/mL crude peanut extract (Stallergenes Greer) with or without 50 ug/mL anti-human IL-4Ra (R&D Systems), as previously described (17).
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bioRxiv - Cell Biology 2023Quote: Angiogenic regulators profiled in the conditioned medium were analyzed using a Proteome Profiler Human Angiogenesis Array Kit (ARY007; R&D Systems). The conditioned medium (1 mL ...
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bioRxiv - Neuroscience 2023Quote: Serum levels of Fgf2 were assessed with the Quantikine® Human FGF2 ELISA kit from R&D systems (Cat. No. DFB50), following manufacturers instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... IL-1β treatment was performed by adding the recombinant human IL-1β protein (R&D Systems, Cat. No. 201-LB/CF) to growth factor-enriched ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 mg/ml of purified recombinant human complement component C5a (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Bioengineering 2024Quote: ... 5% CO2 in R10 containing 60 IU mL-1 recombinant human IL-2 (R&D Systems, CAT#202-IL-059/CF). The following day ...
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bioRxiv - Physiology 2024Quote: ... Slices were then stained individually in 8- well Ibidi dishes with anti-ENTPD3 (R&D Systems Human CD39L3/ENTPD3 Antibody AF4400) to identify beta cells ...
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bioRxiv - Physiology 2024Quote: ... The platelet-poor plasma obtained before and immediately after running were subsequently analyzed for pro– and mBDNF levels using Enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Human BDNF Quantikine Immunoassay 8DBD00, R&D Systems and Human Pro-BDNF ELISA ...
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bioRxiv - Immunology 2024Quote: To detect TGFβ1 released levels in conditioned media from all culture conditions we used a Human/Mouse TGFβ1 Quantikine ELISA kit (R&D systems). Culture conditioned media from all conditions were harvested after 5 days and frozen at -80°C until the assay ...
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bioRxiv - Microbiology 2024Quote: ... The level of SLPI in the serum was measured using the Human SLPI DuoSet ELISA kit (R&D Systems, #DY1274-05).
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bioRxiv - Physiology 2024Quote: ... (1980) and measured using a commercially available ELISA kit (Human IGF-I/IGF-1 DuoSet ELISA, DY291; R&D Systems, Inc.). All samples were assayed in duplicate and final concentrations were adjusted based on the change in plasma volume measured from baseline (18 ...
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bioRxiv - Immunology 2024Quote: Measurement of AREG and HB-EGF in patient serum was performed by ELISA according to the manufacturer’s instructions (Human Amphiregulin Quantikine ELISA Kit, R&D Systems, DAR00 and Human HB-EGF DuoSet ELISA ...
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bioRxiv - Immunology 2024Quote: ... M1 and M2 macrophages was measured using Luminex® (Human Magnetic Luminex® Assays Premixed Multiplex, R&D Systems, LXSAHM-07) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... The supernatants were used with the Human Phospho-EphB4 DuoSet IC ELISA kit (#DYC4057-2, R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Mycoplasma testing was performed every 3 months with the MycoProbe mycoplasma detection kit from R&D systems (Minneapolis, MN). None of the cell lines used in this study are found on the misidentified cell lines list from the International Cell Line Authentication Committee.
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bioRxiv - Cancer Biology 2021Quote: ... Negative control cells were produced by treatment with 10 μM Z-DEVD-FMK caspase-3 inhibitor (R&D systems). 200μl of cell suspension from each cell line including the negative control group was transferred into a flow cytometry tube before being treated with 2.5μl of 0.2mM NucViewTM 488 caspase-3 substrate (fluorogenic DNA dye and DEVD moiety substrate for caspase-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for Mycoplasma routinely every 3 months using MycoProbe Mycoplasma Detection Kit (R&D Systems).
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Immunology 2024Quote: ... and Cl13-infected mice was measured by Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit (R&D Systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Five million of splenocytes per well in 12-well plates were cultured in 2.5 ml of complete DMEM medium (for details refer below) with 1000 U/ml of recombinant human (rh) IL-2 (202-IL-010, R&D Systems) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... To induce WNT and BMP4 activation we additionally supplemented the above protocol with CHIR99021 and/or Recombinant Human BMP4 Protein (R&D Systems) (figure 3A) ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Genetics 2021Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Human serum GzmA levels from healthy donors and patients with confirmed abdominal sepsis at diagnose and during the first 72h was monitored by a commercial ELISA kit (Human Granzyme A DuoSet ELISA, R&D Systems) following the manufacturer recommendations.
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bioRxiv - Developmental Biology 2020Quote: ... An NRG1-β1 ELISA assay was performed according to the protocol of the manufacturer (Human NRG1-β1 DuoSet ELISA, R&D systems). Absolute NRG1-β1 concentration was calculated based on the standard curve.
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bioRxiv - Physiology 2020Quote: ... postnatal C57BL/6J mice at age of day 20 were intraperitoneally (I.P.) injected with recombinant human NTF3 (50μg/kg body weight, R&D Systems, Minneapolis, MN) daily for 10 days ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Microbiology 2021Quote: The relative levels of selected human cytokines and chemokines was determined in parallel using a membrane-based antibody array (ARY005B, R&D Systems). Cells were seeded at 7.5 × 105 cells per well in a tissue-culture treated six-well plate ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...