Labshake search
Citations for R&D Systems :
1851 - 1900 of 2336 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... then coated with indicated concentrations of recombinant human THSD7A overnight (diluted in sterile PBS) (R&D Systems Cat# 9524-TH). Cells were seeded onto THSD7A-coated coverslips and fixed and stained 24 hours post-seeding ...
-
bioRxiv - Cell Biology 2024Quote: ... Conditioned medium samples were subjected to analysis using the Human XL Cytokine Array Kit (R&D Systems, Inc., Minneapolis, MN)per manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). The supernatant and organoid lysates were collected to measure GLP-1 concentrations in secretion and total protein concentrations ...
-
bioRxiv - Immunology 2024Quote: ... Supernatants were harvested and MICA levels were quantified via sandwich ELISA (R&D Systems, DUO Set Human MICA DY 1300). Cells were detached using Versene (Gibco ...
-
bioRxiv - Molecular Biology 2024Quote: Soluble TNFSF14 was measured in murine and human BALF samples using commercially available ELISA kits (murine TNFSF14: R&D Systems, cat ...
-
bioRxiv - Immunology 2024Quote: ... and probed at 4°C overnight with specific antibodies directed against human ACE2 (0.4 μg/mL; R&D Systems, MAB10823) or Mouse ACE2 (0.25 μg/mL ...
-
bioRxiv - Immunology 2024Quote: ... They were stained overnight at 4°C with mouse anti-human PLSCR1 monoclonal antibody (1:50 dilution, R&D Systems) and rabbit anti-human IFN-λR1 polyclonal antibody (1:100 dilution ...
-
bioRxiv - Biochemistry 2024Quote: ... Commercially available recombinant human ubiquitin mutants used in this study included (R&D Systems, UM-K48R, UM-K480, UM-NOK). L8A ubiquitin was purified as described previously 44 ...
-
bioRxiv - Cell Biology 2024Quote: Secreted DPP4 was detected from supernatants 48 h after feeding using the human DPP4/CD26 DuoSet ELISA (R&D Systems) as described by the manufacturer ...
-
bioRxiv - Cell Biology 2024Quote: ... and human collagen I alpha 1 were assessed utilizing magnetic Luminex® Assays (R&D systems; #LXSARM-03 / #LXSAHM-04). The secretome samples used for these assays consisted of three samples from each culturing group that were pooled (n = 3) ...
-
bioRxiv - Microbiology 2021Quote: ... cells were maintained in SFD media supplemented with the following: 3 μM CHIR 99021 (CHIR, R&D Systems), 10 ng/ml human FGF10 ...
-
bioRxiv - Cell Biology 2023Quote: ... after which the cells were exposed for 3 days to 200 ng/ml FGF-9 (R&D systems) and 1μg/mL heparin (Sigma Aldrich ...
-
bioRxiv - Developmental Biology 2024Quote: ... and incubated with rabbit active Caspase-3 (5 μg/ml, AF835-SP, R&D systems, Inc., Minneapolis, MN), rabbit cleaved Caspase-9 (1:50 ...
-
bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
-
bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant human IL-21 was from Preprotech (200-21) and IL-21 neutralizing antibody was from R&D Systems (991-R2).
-
bioRxiv - Immunology 2020Quote: ... culture supernatants were collected and cytokine concentrations measured using CXCL10/IP10 (Human CXCL10/IP-10 Quantikine ELISA Kit, R&D Systems) and IFN-β (VeriKine-HS Human IFN Beta Serum ELISA Kit Cat No 41415 ...
-
bioRxiv - Bioengineering 2021Quote: ... Serum IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
-
bioRxiv - Neuroscience 2021Quote: Human soluble E-selectin levels were assayed using quantitative sandwich enzyme immunoassay technique and following the manufacturer’s protocol (Quantikine ELISA Human E-selectin/CD62E Immunoassay, DSLE00, R&D Systems). Briefly ...
-
bioRxiv - Neuroscience 2020Quote: ... or the indicated compounds were added at 12.5µM and incubated for 30 min at room temperature prior to adding biotinylated human VEGF-A165 (Cat#BT293, R&D systems) at 10 nM ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were later determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
-
bioRxiv - Neuroscience 2021Quote: ... Sigma-Aldrich)-coated dishes in DMEM/F12/ B27/N2-medium supplemented with 200 ng/ml human recombinant sonic hedgehog (SHH, R&D Systems) and 100 ng/ml murine recombinant fibroblast growth factor 8b (FGF-8b ...
-
bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
-
bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
-
bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
-
bioRxiv - Cell Biology 2022Quote: ... Secretion of angiogenesis-related protein levels was detected using a Proteome Profiler™ Human Angiogenesis Antibody Array kit (R&D Systems) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Group 2 ligands were derived from human transcriptomic data and included: TGFβ2 (R&D Systems, Cat. 302-B2-002, 5ng/mL), NLGN1 (Sino Biological ...
-
bioRxiv - Immunology 2022Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
-
bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
-
bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
-
bioRxiv - Cancer Biology 2020Quote: ... neutralizing Ab anti-human/mouse SDF-1 25μg/ml (MAB310) and mouse VEGF 150ng/ml (VEGF164) (all from R&D System).
-
bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed twice in PBS and stained with a goat anti-human ACE2 polyclonal antibody (R&D Systems, Cat # AF933) or isotype goat polyclonal isotype control (R&D Systems ...
-
bioRxiv - Microbiology 2020Quote: ... the wells were washed and then incubated with human SP-D detecting antibody (0.5 μg/ml) (R&D Systems; 1:180) for 2 h at room temperature ...
-
bioRxiv - Immunology 2020Quote: Chemokine and cytokine protein quantification were performed using Proteome Human and Mouse Cytokine Array kits (R&D Systems, San Diego, CA) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Cell culture supernatant levels of IFNg were quantified using Human IFN-gamma Quantikine ELISA Kit and manufacturer’s recommendations (DIF50, R&D Systems).
-
bioRxiv - Immunology 2020Quote: Cytokines and chemokines were measured using Human Magnetic Luminex Assay according to the manufacturer’s instructions (#LKTM014, R&D systems, MN, USA). Samples that were above the upper limit of quantification were set at the upper detection limit of the assay ...
-
bioRxiv - Cancer Biology 2022Quote: ... 300 μg cell lysates were used for phosphorylation analysis with Human Phospho-Kinase Array Kit (ARY003B, R&D Systems, Minneapolis, MN) following the manufacturer’s instruction.
-
bioRxiv - Bioengineering 2022Quote: The presence of selected cytokines was evaluated in samples of conditioned medium with Proteome profiler human angiogenesis array (R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: Supernatants from transfected HEK293T cells were analyzed for their LBP content by Human LBP DuoSet ELISA (#DY870-05, R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Purified recombinant NRX1β ectodomain fused to human immunogloblin Fc (NRX1β-Fc, R&D sytems, Cat#5268-NX-05, R&D system) was immobilized on carboxymethylated dextran CM5 sensor chips (Cytiva ...
-
bioRxiv - Physiology 2023Quote: ... Plasma and urine GDF15 were measured by ELISA test (MG150 and DGD150, for rodent and human GDF15, respectively, R&D Systems) and tissue GDF15 was measured using an ELISA test (Elabscience).
-
bioRxiv - Neuroscience 2022Quote: ... the media was incubated (72 h) with media containing different concentration of recombinant human PTN (rhPTN, R&D systems 252 - PL) or the drugs alectinib (Toronto Research Chemicals ...
-
bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
-
bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
-
bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
-
bioRxiv - Neuroscience 2024Quote: ... were incubated for 2 h at room temperature (RT) with biotinylated polyclonal goat anti-human TREM2 capture antibody (0.1 μg/ml; AF1828, R&D Systems). Following incubation with streptavidin-HRP (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... Monocytes were differentiated into hMDMs for at least 7 days in complete medium containing 10 ng.mL-1 of recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems).