Labshake search
Citations for R&D Systems :
1901 - 1950 of 1955 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... was then diluted in water to 50 uL in a 96-well microtiter plate and 10 uL Malachite Green Reagent A was added per manufacturer instructions (R&D Systems Cat# DY966). After 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were administered either a 5 μL volume of phosphate-buffered saline (PBS) containing 10 μg/kg of carrier-free rmIL-1β (R&D Systems, Minneapolis, MN) or PBS alone (control ...
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bioRxiv - Cell Biology 2019Quote: ... secreted IL-2 samples were diluted 1:10 and subsequently analyzed using Mouse IL-2 Quantikine ELISA Kit (R&D Systems Inc, Minneapolis, MN), as per manufacturer’s specifications.
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bioRxiv - Neuroscience 2020Quote: ... L-glutamine and 10 ng/ml of carrier-free murine recombinant granulocyte and macrophage colony-stimulating factor (GM-CSF, 415-ML, R&D systems, Minneapolis, MN). Cell suspension from one brain was plated in a T75-cell culture flask coated with poly-D-lysine (P7280 ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with fresh media (DMEM + 10% FBS) supplemented with 0.03 ng ml−1 of recombinant mouse IFNγ (R&D Systems, #485-MI-100). After 24 h treatment ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of IP10 in the media was then determined by ELISA using the Mouse CXCL10/IP-10/CRG2 DuoSet ELISA from R&D system (cat. DY466) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Cell Biology 2021Quote: ... directly after UV irradiation or pre-treated 1 hour before irradiation with proteasome inhibitor MG132 (Enzo, 50 µM) or NEDD8 E1 Activating Enzyme Inhibitor (NAEi) MLN4924 (R&D systems, 10 µM) where indicated ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 were assayed using a Luminex mouse pre-mixed Multi-Analyte kit according to standard instructions (LXSARM-06, R&D System, Minnesota, USA).
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in appropriate cell culture medium as directed by supplier with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems; Minneapolis, MN) and 1% Penicillin-Streptomycin (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Seven different ligand conditions were tested at concentrations previously determined to elicit maximal cell responses49 (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM) ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes from SP and draining LNs were cultured in the presence of IRBP-p (10 μg/ml) and IL-23 (R&D systems, Minneapolis, MN) (10 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C in 5% CO2 in a humidified incubator in IMDM with phenol red supplemented with 10% fetal bovine serum (FBS) (R&D systems, Minneapolis, MN), 1mM Sodium Pyruvate ...
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Immunology 2023Quote: Concentrations of CXCL10 in K18-hACE2 mice were measured in serum and lung supernatant collected at euthanasia using the Mouse Magnetic Luminex Assay kit for mouse CXCL10/IP-10/CRG2 (R&D Systems LXSAMSM-01). Both serum and supernatant samples were diluted 1:2 in the kit’s Calibrator Diluent RD6-52 then utilized in the assay procedure provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Myeloperoxidase (MPO, indicative of tissue oxidation) levels in ALF (using 10 μg of protein) were determined by using LUMINEX (R&D Systems, Minneapolis, MN).
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bioRxiv - Neuroscience 2023Quote: ... After transfection neurons were washed twice with NBP and treated with 20 μM (2R)-amino-5-phosphonovaleric acid (AP5; R&D Systems, 0105/10), an NMDA receptor antagonist to prevent transfection-induced neuronal overexcitation ...
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bioRxiv - Physiology 2019Quote: ... Cells were washed with BEC medium containing 10% FBS and were re-suspended in Flow Cytometry Staining Buffer (1X) (R&D Systems, Minneapolis, MN, USA) supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse SCLC cells were seeded into 6-well plates and cultured for 16 h in RPMI1640 + 10% BGS with or without 100 ng/mL of Wnt5a (R&D System, 645-WN-010) or recombinant Wnt3a (PeproTech ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Cell Biology 2021Quote: ... For differentiation assays cells were cultured in differentiation media (DMEM + 1% FBS + 1% P/S) with or without recombinant TGFβ (R&D systems, 10 ng/mL) and analyzed 72 hours later by immunofluorescent staining described below ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed with full media containing FGF (10 ng/ml bFGF, Sigma Aldrich #F0291; 25 ng/ml rhFGF, R&D Systems #345-FG), BMP-4 (40 ng/ml ...
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bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...
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bioRxiv - Genomics 2024Quote: ... The human RPTEC with early passages were seeded at 8 × 105 cells on 10 cm culture dish at the day before the assay and treated with or without TNFα (R&D systems, 100 ng/ml) at 3 h before the fixation ...
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bioRxiv - Microbiology 2022Quote: ... 5×105 isolated splenocytes were cocultured with different stimuli in 200 μL of RPMI-10% FBS complete medium for 48 h on IFN-γ-coated plates (Cat# EL485, R&D systems, Minneapolis, USA). Fifteen-mer overlapping peptides from SARS-CoV-2 spike glycoprotein (Cat# PM-WCPV-S-1 ...
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Genetics 2023Quote: Reprogramming was induced in the presence or absence of IFNγ at 10 ng/ml (from day 0 to 5) (R&D Systems, 485-MI-100). SSEA1+ day 5 iPSCs (4 replicates from different reprogramming rounds ...
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bioRxiv - Pathology 2023Quote: ... CXCL2, CXCL1, TNF, MMP8, S100A8, IFN-γ, IL12p40, IL-12p70, IL-10, and VEGF using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
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bioRxiv - Systems Biology 2023Quote: ... The assays with TNF pre-conditioning were performed identically except that HBMECs were activated with 10 ng/ml TNF (R&D Systems Cat# 10291-TA) for ∼22 hours before media equilibration ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Bioengineering 2023Quote: VeroE6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (ThermoScientific, Waltham, MA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Bio-Techne, R&D systems, Minneapolis, MN, USA) and 1% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... 2×105 cells were cultured in CH12 medium supplemented with a mixture of IL4 (10 ng/mL, R&D Systems #404-ML-050, Minneapolis, MN, USA), TGFβ (1 ng/mL ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 2% fetal bovine serum for 6 hours and then left untreated or treated with 10 ng/ml IL-17A or 20 ng/ml IL-36γ alone or with the addition of 10 ng/ml TNF-α (R&D Systems, Minneapolis, MN, USA) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were starved in a serum-free high pH medium and then incubated overnight with a cocktail of 10 ng/ml TNFα (R&D Systems, 210-TA-100/CF) and 50 ng/ml IL-17A (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... and 10 µg/mL of mAb 18B7 in feeding medium using the Total NO and Nitrate/Nitrite Parameter (R&D Systems; detection limit, 0.78 μmol/L) and Griess Reagent method (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... for 5 days followed by a further 15 days in Stage 2b media (HCM containing 10 𝜇g/mL Oncostatin M (R&D Systems Cat No. 295-OM) and 10nM dexamethasone).