Labshake search
Citations for R&D Systems :
1851 - 1900 of 1955 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Wisent Inc., 319-005-CL) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, S12450) and 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Immunology 2023Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of rhuIL-2 (10 U/mL, R&D System) to expand the T cells ...
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Neuroscience 2023Quote: ... or bFGF (10 ng/mL) and diazepam (100 μM) (045-18901, Wako) or BMP4 (50 ng/mL) (5020-BP, R&D Systems) (quiescence medium ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
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bioRxiv - Genetics 2023Quote: Reprogramming was done as described previously, combining the IFNγ (10 ng/ml, day 0 to 5) (R&D Systems, 485-MI-100) with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM ...
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bioRxiv - Microbiology 2023Quote: PLY-treated H292 monolayers or PMNs in suspension were treated with 1U PLY and incubated for 30 min at 37 °C with 10 μM fluorogenic peptide MMP substrate (Mca-PLAQAV-Dpa-RSSSR-NH2, R&D Systems) diluted in 25 mM Tris buffer ...
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bioRxiv - Bioengineering 2023Quote: ... The classical monocytes were isolated using biotin-conjugated antibodies and streptavidin-conjugated magnetic beads to deplete labeled cells and were then conditioned into resting macrophages (M0) using RPMI 1640 supplemented with 10% fetal bovine serum (FBS, S11150H, R&D Systems) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Cell Biology 2023Quote: BeWo cells were purchased from ATCC and maintained in culture with equal parts F12K (ATCC) (supplemented with 10% Fetal Bovine Serum (R&D Systems), Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was aspirated and the pellet re-suspended in fresh E8 medium supplemented with 10 µM ROCK inhibitor (Tocris R&D Systems). Re-suspended cells were seeded at a density between 7,500 – 12,500 cells / cm2 on fresh Matrigel-coated plates (Day – 3) ...
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bioRxiv - Immunology 2022Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Microbiology 2024Quote: ... were seeded on 18 T175 flasks in Eagle’s Minimum Essential Medium (EMEM; ATCC 30-2003) supplemented with 10% FBS (R&D Systems S11150H) and 100 U/mL of penicillin-streptomycin (Gibco 15140122 ...
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bioRxiv - Molecular Biology 2024Quote: ... J774.A1 cells (ATCC, Gaithersburg, MD-US) were seeded in complete media, composed by DMEM (Cytiva, Marlborough, MA-US) and 10% FBS (R&D Systems) in CO2 incubators at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... then maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems). For the pilot experiment in which CD4 cells with a defined CAR BC were spiked into CD8 cells (Figure 1D-F) ...
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bioRxiv - Immunology 2024Quote: ... All primary cells were maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems) at 37°C in 5% CO2.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... M-CSF protein levels were determined in bone marrow supernatants (obtained by centrifugation at 1000 g for 10 minutes) or in cell culture medium using Quantikine ELISA kits (R&D Systems). Results obtained with bone marrow supernatants were normalized to total protein levels.
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bioRxiv - Cancer Biology 2022Quote: ... media in the wells was either with or without 10% FBS and 1μg/mL secreted N-SLIT2 (R&D Systems, 8616-SL-050). The cells were incubated in the Matrigel-coated inserts for overnight in the incubator ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC, Manassas, Virginia; Cat #30-2002) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, Minneapolis, MN), 1% penicillin/streptomycin (PS ...
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bioRxiv - Cell Biology 2019Quote: ... 2 ug/ml Doxycycline and 3.34 ug/ml Puromycin, 1 × N27 supplement, and 10 ng/ml BDNF (STEMCELL, 78005) and GDNF (R&D Systems, 212-GD)) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
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bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
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bioRxiv - Pathology 2020Quote: ... Cells were cultured to 70-80% confluence and then treated with vehicle (4mM HCl with 0.1% bovine serum albumin) or 10 ng/mL TGF-β1 (Cat#: 7754-BH-005, R&D Systems, Minneapolis, MN) or 10 ng/mL TNF-α (Cat#210-TA-020 ...
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bioRxiv - Microbiology 2021Quote: ... were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (Bio-Techne, R&D systems, Minneapolis, MN, USA) and 1% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then seeded onto 24 well plates (106 cells/well) with or without 10 ng/mL murine IFN-γ (R&D Systems). Sixteen hours later ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 1μg of mouse serum IgG for 10 min at RT then incubated with: AF-488 mouse anti-human ACE2 (R&D systems, FAB9333G), APC mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Pathology 2022Quote: ... was seeded with 10 000 myoblasts in serum-free medium while the wells were filled with either proliferating medium or serum-free medium complemented with 10 ng.mL-1 FGF-b (R&D Systems 3139-FB), IGF-I (R&D Systems 791-MG ...
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bioRxiv - Cell Biology 2022Quote: ... 100μg of GST-NEDD4△52 was bound to 10 μl Glutathione sepharose resin and incubated with 50 nM ubiquitin activating enzyme UBA1 (R&D Systems, E305025), 750 nM ubiquitin conjugating enzyme UBCH5B (R&D Systems ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: VeroE6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (Bio-Techne, R&D systems, Minneapolis, MN, USA) and 1% (v/v ...
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bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
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bioRxiv - Genomics 2024Quote: ... Progenitors were then resuspended in a 10% dilution in volume of FBS and 100ng/mL CSF-1 (R&D Systems; 216-MC-500) in Gibco™ RPMI-1640 media (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Lymphatics were visualized by injecting 0.4ug of Mouse LYVE-1 Alexa Fluor® 594-conjugated Antibody (20ul of concentration 20ug/ml, diluted 1:10 in normal saline; cat# FAB2125T; R&D systems) into the tongue in a region near the tumor 20 minutes before imaging ...
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were cultured in RPIM 1640 medium containing 20% porcine serum and 10 ng/mL recombinant porcine granulocyte-macrophage colony-stimulating factor (GM-CSF, R&D Systems, UK) to differentiate mature bone marrow-derived macrophage (BMDMs ...
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bioRxiv - Immunology 2023Quote: iBMDMs were maintained in Dulbecco’s Modified Eagle Medium (DMEM; HyClone Cytiva, Cat no. SH30243.01) supplemented with 10% fetal bovine serum (FBS) (R&D Systems, Cat no. S11550). iBMDMs were passaged once they reached 70–90% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Cancer Biology 2023Quote: ... TUBO and TUBO/Crimson were cultured using high-glucose DMEM base medium with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems, S11150H), 10% NCTC-109 medium (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/ml PDGF-AA (PDGFRα homodimer cells) or PDGF-BB (PDGFRα/β heterodimer and PDGFRβ homodimer cells) ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cell Biology 2023Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60–70% confluence or PDGFRα/β heterodimer cells at ∼70-80% confluence were serum starved for 24 h in HITES medium and stimulated with 10 ng/ml PDGF-AA (PDGFRα homodimer cells) or PDGF-BB (PDGFRα/β heterodimer and PDGFRβ homodimer cells) ligand (R&D Systems) for the indicated length of time ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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bioRxiv - Immunology 2023Quote: ... Parallel experiments included the addition of a neutralizing anti-IL-15 MoAb (10 mg/ml) (mab247, mouse IgG1, R&D systems, Abingdon, UK). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with assay medium supplemented with 10 ng/ml transforming growth factor-beta 1 (hereafter referred to as TGF-β, 240-B-002, R&D Systems) for 24 h or 72 h (Peinado et al. ...
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bioRxiv - Immunology 2024Quote: Isolated B cells were activated at a concentration of 2E6 cells/mL with 10 ng/mL rBAFF (R&D systems 8876-BF-01) and specified combinations of 5 µg/mL (unless otherwise stated ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with fresh media (DMEM + 10% FBS) supplemented with 0.03 ng ml−1 of recombinant mouse IFNγ (R&D Systems, #485-MI-100). Total RNA from untreated cells (24 h ...