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Citations for R&D Systems :
1751 - 1800 of 2579 citations for Rat Dual Specificity Mitogen Activated Protein Kinase Kinase 7 MAP2K7 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and WNT16 levels were determined in senescent fibroblast lysates using a standardized sandwich Enzyme-Linked Immunoassay (ELISA) technique (R&D Systems, Minneapolis, MN, USA), according to manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants or sulcus fluid from patients with different disease stage were evaluated for MMP-9 content using ELISA Duo Set (DY911, R&D System, Wiesbaden, Germany.) following the manufactureŕs protocol.
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested to measure the release of IFN-γ using a IFN-γ-specific ELISA according to the manufacturer’s instructions (R&D Systems, DY28B05). To evaluate T cell activation markers ...
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bioRxiv - Bioengineering 2023Quote: ... IP-10/CXCL10 release from mouse cells was assayed using the Quantikine Mouse IP-10 ELISA assay (R&D Systems, Catalog No. DY466-05) using cell-free supernatants of stimulated cells according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytokine secretion was carried out as a validation from the results of the human cytokine array using a range of human ELISAS from R&D Systems (see below).
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bioRxiv - Physiology 2024Quote: ... 50 µL of murine plasma was then used to quantify glucagon concentrations with ELISA by following the manufacturer’s protocol (R&D Systems, cat no. DGCG0). Final glucagon concentrations were calculated based on a standard curve.
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bioRxiv - Molecular Biology 2024Quote: ... The secretion of IFN-γ was also quantified in cell supernatants using a sandwich mouse IFN-γ Duo Set ELISA (DY485-05, R&D systems). Standards and samples were diluted appropriately to obtain results within the stated detection levels.
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bioRxiv - Neuroscience 2021Quote: ... and with BSA as a carrier protein to enhance protein stability for in-vitro studies (R&D Systems; 458-MF). Leptin was obtained from the National Hormone and Peptide Program (Dr ...
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bioRxiv - Molecular Biology 2023Quote: The recombinant human transforming growth factor beta (TGFβ) protein and recombinant human TSP2 protein were obtained from R&D Systems. The SMAD3 Inhibitor SIS3 was procured from Cayman Chemical Company ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... The tissue section slides were fixed and stained with a rat anti-intelectin monoclonal antibody (R&D Systems, Clone 746420) with fluorescent detection and DAPI (ProLong antifade reagent with DAPI) ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.
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bioRxiv - Biochemistry 2023Quote: ... were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37°C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs were incorporated into a 3D matrix made of rat tail type 1 collagen (R&D Systems) as previously described 95 ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1.7 ng hrWnt11 protein (R&D Systems) or BSA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse RANKL protein (R&D systems, 462-TEC), mouse M-CSF (R&D systems ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant protein of mouse BMP4 (R&D Systems) was added to the medium 16 h before analysis.
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bioRxiv - Cell Biology 2020Quote: ... Tamm-Horsfall protein (cat# AF5144, R&D Systems), CD45 (clone HI30,cat# 304002,) ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant FREM1 protein (R&D Systems, Minneapolis, MN) dissolved in DPBS was diluted to final concentrations of 7.5 µg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant FREM1 protein (R&D Systems, Minneapolis, MN) dissolved in DPBS was diluted to final concentrations of 7.5 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... or ErBb2-Fc fusion proteins (R&D Systems) for 30 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant proteins were purchased from R&D Systems: EDA-A1 (3944-ED) ...
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bioRxiv - Cell Biology 2023Quote: ... where recombinant Wnt5a protein (rWnt5a, R&D Systems) is added to ENR at a final concentration of 200 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Systems Biology 2019Quote: ... Sera were subjected to depletion of abundant serum proteins using Proteome Purify 12 Human Serum Protein Immunodepletion Resin (R&D Systems). Denaturing was followed by alkylation with N-ethylmaleimide and acetone precipitation ...
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bioRxiv - Cancer Biology 2024Quote: Magnetic beads were coated with recombinant human P-cadherin/Fc chimera protein or recombinant human IgG1 Fc protein (R&D systems) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2019Quote: ... 5.0 × 105 PCs and FBs were grown in a single well of a 24-well dish for 24 hours and enzyme-linked immunosorbent assay (ELISA) was performed per manufacture protocol (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Molecular Biology 2020Quote: Hyaluronic acid of COVID-19 patients’ plasma was measured in 1:5 dilution by the enzyme-linked sandwich assay Hyaluronan DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s descriptions ...
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bioRxiv - Pathology 2020Quote: ... TNF-α and IL-1β) in the frozen distal small intestine and colon were measured by porcine specific ELISA assays (R&D Systems, Abingdon, UK).
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bioRxiv - Physiology 2019Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their co-incubation with T2 cells pulsed with MART-126–35 (commercially synthesized and purified [>95%] by reverse-phase HPLC by Biomer Technology, Cheshire, UK) by ELISA (R&D Systems, Minneapolis, MN, USA). The MART-126–35–-reactive clones were further expanded in a second-round of exposure to 30 ng/ml anti-CD3 ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50 ...
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: CCL-149 rat lung epithelial cells (ATCC) were cultured in DMEM/F12 with 10% fetal bovine serum (FBS, R&D Systems) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Developmental Biology 2021Quote: ... MSD standard-bind microtitre plates were first coated with 30µl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 µg/ml in PBS ...
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bioRxiv - Neuroscience 2021Quote: Single-cell RNA-seq analysis of cultured NSCs isolated from the cortex of E14.5 Sprague-Dawley rats (NSC001, R&D systems; cultured according to the manufactures’ protocol ...