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Citations for R&D Systems :
1701 - 1750 of 2579 citations for Rat Dual Specificity Mitogen Activated Protein Kinase Kinase 7 MAP2K7 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... IL-8 and TNF-α were quantified in the supernatants from uninfected and SARS-CoV-2-infected hiPSC-CMs by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CCL22/Ccl22 ELISAs were performed on culture supernatants (undiluted or diluted when necessary) using Immulon 4HBX flat bottom microtiter plates with the human CCL22/MDC DuoSet ELISA (R&D Systems DY336) or the mouse Ccl22/Mdc DuoSet ELISA (R&D Systems DY439 ...
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bioRxiv - Immunology 2019Quote: The p24 ELISA with supernatant from infected and non-infected LPMC cultures was performed according to the manufacturer’s protocol (R&D Systems, Minneapolis, USA). Absorbance was measured using the Spark® 10M multimode microplate reader (Tecan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in BALF were quantified by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol (DY406, DY410 and DY401 from R&D systems; Minnesota, USA). The UV absorbance at 450 nm and 570 nm were measured for the primary signal and for plate correction ...
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bioRxiv - Physiology 2021Quote: ... IL-1β and IL-1α concentrations in conditioned media were evaluated using Human IL1-β and IL-1α ELISA assays (DY201 and DY200, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and Ccl19/Ccl21 levels were determined by indirect sandwich of enzyme-linked immunosorbent assay (ELISA) following manufacturer’s instructions (R&D Systems, Minneapolis, MN). Multi-analytic profiling beads using Milliplex® Multiplex mouse cytokine/chemokine panel I kit (EMD Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... The concentration of hCG-β in the culture medium was measured using the Human CG beta (HCG beta) Duoset ELISA (#DY9034-05, R&D Systems) according to the manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Levels of vascular endothelial growth factor (VEGF) were evaluated by sandwich enzyme-linked immunosorbent assay (ELISA) in GL261 cell supernatants using matched antibody pairs from R&D Systems (USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 was measured by ELISA (enzyme-linked immunosorbent assay) with the obtained supernatant and analyzed according to the manufacturer’s protocol (R&D Systems, Minnesota, USA).
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Doses were previously optimized and mice were intravenously injected with 5×1010 genome copies of both Control- and TSLP-AAV for 14 days with serum TSLP levels confirmed using a murine specific ELISA (R&D Systems MTLP00)4.
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bioRxiv - Cell Biology 2023Quote: ... plasma and tissues of interest were collected at different post-infection times and assessed by ELISA using commercially available antibodies and according to the procedures supplied by the manufacturer (R&D Systems, Minneapolis). All samples were stored at -20°C until processing ...
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bioRxiv - Physiology 2020Quote: Plamatic IgE level was evaluated by SDS PAGE using rat anti-mouse IgE antibodies (R&D systems Cat# MAB9935).
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bioRxiv - Molecular Biology 2020Quote: ... Goat anti-SOX17 (cat# AF1924) and rat anti-SSEA3 (clone MC-38, cat# MAB1434) were from R&D Systems. Mouse anti-CD184 (CXCR4) ...
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bioRxiv - Physiology 2022Quote: ... mouse IgG2a kappa (eBM2a) (eBioscience™, 14-4724-82) and the rat IgG (R&D Systems, 6- 001-F).
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... starved for 2 hours and treated with 10 ng/ml rat recombinant EGF (R&D Systems, Minneapolis, MN, USA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used in the study: anti-rat monoclonal S100A8 (Cat: MAB3059; R&D Systems; Minneapolis, MN) and anti-goat Iba1 (Cat ...
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bioRxiv - Immunology 2023Quote: ... a 50:50 mixture of Matrigel Matrix GF and 4 mg/ml Cultrex Rat Collagen I (R&D Systems). For time-lapse live imaging experiments ...
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bioRxiv - Cell Biology 2023Quote: Microfluidic devices were prepared as described previously [32] using 2.5 mg/mL Collagen I (rat tail, R&D Systems) as extracellular matrix ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Neuroscience 2024Quote: ... was assessed on 5μL murine plasma using the human SimplePlex Assay as the kit is cross reactive with murine NF-H (R&D Systems, Protein Simple SPCKB-PB-000519) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems) in tissue culture flasks ...
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Immunology 2019Quote: ... Tissue was washed and when needed incubated with secondary fluorescently labeled antibodies (Donkey Anti-Rabbit Pacific Blue or Alexa Fluor 647) for 2h before nuclear staining with Draq-7 (R&D Systems) or 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Immunology 2020Quote: Intestinal tissues from mice treated with PBS or spore for 7 d and 45 d were lysed with cell lysis buffer (R&D Systems) supplemented with 1% 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
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bioRxiv - Immunology 2022Quote: ... Sorted cells were plated at 5000 cells per well in a 96 well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant Biotin-tagged K48-linked poly-ubiquitin chains (2-7) and K48-linked poly-ubiquitin chains (2-16) were purchased from R&D Systems and Enzo Life Science ...
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bioRxiv - Immunology 2023Quote: ... Sorted cells were plated at 5000 cells per well in a 96-well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... over the course of 7 additional days with 25 ng/mL M-CSF and 25 ng/mL RANKL (both from R&D Systems), as previously described [17,30,49] ...
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bioRxiv - Neuroscience 2019Quote: CXCL12 concentration in supernatants of CXCL12 Ch/DS NC was quantified using Mouse CXCL12 DuoSet ELISA as per supplier instructions (R&D systems, Minneapolis, USA). Encapsulation efficiency and drug loading were calculated as follow:
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bioRxiv - Cancer Biology 2022Quote: The levels of murine TRAIL/TNFSF10 (DY1121) in the cells were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (R&D Systems, Emoriville, California, USA). The OD value was measured by Microplate Reader (Multiskan Spectrum ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
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bioRxiv - Pathology 2020Quote: ... The culture media was then harvested and the concentrations of human IL-1β and IL10 were measured using ELISAs (DY201 and DY217B, R&D Systems, Oakville, ON).
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bioRxiv - Immunology 2019Quote: ... and the tissue levels of myeloperoxidase (MPO) in the supernatants were determined using a mouse MPO ELISA (R&D systems, Biotechne, Minneapolis, MN, USA). The fixed tissues (left lungs ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Neuroscience 2022Quote: ... we determined the amount of HB-EGF per cell through enzyme linked immunosorbent assay (ELISA; Cat# DY8239-05, R&D Systems, Minneapolis, MN, USA). For analysis of HB-EGF levels in brain tissue ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).