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Citations for R&D Systems :
1701 - 1750 of 2812 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... ELISA was performed using Human Gas6 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Minneapolis, Minnesota, USA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Cancer Biology 2022Quote: ... Chemokine levels were measured using the Invitrogen ELISA Kit (Quantikine mouse/human CXCL10/IP-10; R&D Systems, Minneapolis, MN) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... with mouse and human soluble FLT1-FC chimeric protein (471-F1-100 and 321-FL-050/CF, R&D Systems) immobilized to about 1,000 RU was used as ligand for the analyte binding of anti-FLT1 MAbs ...
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bioRxiv - Cancer Biology 2020Quote: ... Prostate-specific serum-free culture media contained 500 ng/mL human recombinant R-spondin (R&D Systems, Cat: 10820-904), 10uM SB202190 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718, RRID:AB_2278695; R&D Systems, Inc., Minneapolis, MN, USA); and developed using SuperSignalTM west -pico or -femto chemiluminescence kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human WNT3A (#5036-WN-010) and WNT4 (#6076-WN-005) were obtained from R&D Systems (Minneapolis, MN, USA) and reconstituted per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2021Quote: ... the concentration of amphiregulin in the supernatant was determined using the Human Amphiregulin Quantikine ELISA Kit (R&D Systems, DAR00) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Genetics 2020Quote: Plasma VEGF-A concentration was measured using the quantitative sandwich ELISA kit (Human VEGF Quantikine; R&D Systems, Minneapolis, MN) following the manufacturer’s instructions in our laboratory at low altitude (600 m) ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Immunology 2022Quote: IL-22BP quantification was performed with the Human IL-22BP ELISA DuoSet kit (R&D System, ref DY-1087-05) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Pathology 2023Quote: CCL2 was quantified in conditioned media from HAoSMCs using Human CCL2/MCP-1 Quantikine ELISA Kit (DCP00, R&D systems) according to the manufacture’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.
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bioRxiv - Immunology 2023Quote: ... and in bone marrow plasma using the Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit (R&D Systems).
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bioRxiv - Neuroscience 2023Quote: Levels of soluble VEGF-A were assessed by a Human VEGF-A Quantikine ELISA kit (Bio-techne R&D Systems), according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... MDMs were stimulated in complete RPMI with either 10 ng/mL human IFN-ψ (285-IF-100, R&D Systems) or 50 ng/mL LPS ...
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bioRxiv - Cancer Biology 2023Quote: The levels of INFγ were evaluated in supernatants obtained from 0.625:1 and 1.25:1 effector:target ratio overnight co-cultures of CAR T-cells:CAFs using the Human IFN-Gamma Quantikine Kit (R&D Systems) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiation was induced by addition of human recombinant Activin A (ActA, 30 ng/ml, R&D systems, 338-AC-050).
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and florescent antibody for 15 minutes on ice: PB2 PE (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Immunology 2023Quote: ... Production of immune mediators in the supernatants was assessed using a human 45-plex (R&D Systems, Inc. Minneapolis, Minnesota).
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bioRxiv - Immunology 2023Quote: Supernatants were harvested and cytokine levels were assayed using ELISA kits for human TNF and IL-18 (R&D Systems).
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bioRxiv - Molecular Biology 2023Quote: ... Expression of angiogenic proteins in CM was measured using a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D systems) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Tissue constructs were cultured in the presence of 10ng/ml TGF-β3 (human recombinant active TGF-β3, R&D Systems) for the initial 14 days ...
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bioRxiv - Cell Biology 2023Quote: ... GPNMB levels in the supernatant were measured by ELISA (DuoSet ELISA Development system, Human Osteoactivin/GPNMB, #DY2550, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Microbiology 2023Quote: ... each 25μl of pelleted resin was incubated with 2μg of recombinant human K63-linked (R&D systems, Cat# UC-320) or K48-linked (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... IFN-β amounts in the culture supernatant were measured by the human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... Wells were flooded with BrainPhys media with supplements and 10 ng/mL human NGF (256-GF-100, R&D Systems), and 3 μg/mL FRDU and incubated at 37° C and 5% CO2 until further experimentation.
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Microbiology 2024Quote: Recombinant human IFN-β (8499-IF) and IFN-λ2 (1587-IL) were purchased from R&D Systems (Minneapolis, MN, USA). Both were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: The chemokine CXCL13 was measured in LO chip effluent using a human CXCL13 ELISA assay (Quantikine, R&D Systems #DY801), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used for immunofluorescence in this study were as follows: anti-mouse 1:150 GPR34 (R&D Systems; Cat. No. MAB4617), anti-rabbit 1:1000 IBA1 (Wako ...
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bioRxiv - Molecular Biology 2019Quote: ... of LPS treated rats were double-immunostained for the analysis of the cellular localization of cathepsin X using the following primary antibodies: goat polyclonal anti-cathepsin X antibody (1:75, R&D System), mouse monoclonal anti-TH antibody (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were fixed in an ice-cold acetone/methanol mixture (50%/50%) and stained with primary anti-mouse CD144 antibody (MAB9381, 1:100, R&D Systems) and secondary donkey-anti-mouse Alexa Fluor® 594 (A21203 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... Lung lobes were incubated with primary antibody (anti-VWF, rabbit, 1:100, F3520, Sigma and anti-EMCN, goat, 1:200, AF4666, R&D Systems), in 5% donkey serum and 0.5% Triton X-100 in phosphate buffered saline (PBST ...
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bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Incubation with primary rat-polyclonal antibody against LYVE-1 in combination with rat-polyclonal biotinylated anti-MHC-II antibody (both R&D Systems) was done for 2h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1h at room temperature and probed with primary antibodies against SORCS2 (1:750; AF4238, R&D Systems) and GAPDH (1:10,000 ...