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Citations for R&D Systems :
1601 - 1650 of 2812 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... were coated with 100 μl per well of 1.5 μg/ml human recombinant integrin αvβ6 (R&D Systems) diluted in coating buffer from the ELISA Starter Accessory Kit (Bethyl Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... 10 ng/mL recombinant human transforming growth factor beta 1 (TGF-β1 from R&D System, Minnesota, USA). For 3D cultures ...
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bioRxiv - Bioengineering 2023Quote: ... This was done by incubating 100 µg of recombinant human G-CSFR (381-GR/CF, R&D Systems) in 950 µL PBS for 2 h at room temperature (RT ...
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bioRxiv - Neuroscience 2023Quote: Recombinant Human Neuronal Pentraxin 1 Protein (NP1) was purchased from R&D systems (#: 7707-NP, bio-techne, USA).
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bioRxiv - Cell Biology 2023Quote: ... Levels of protein phosphorylation were then detected using the Human phospho-kinase Array Kit (ARY003B, R&D Systems) according to the manufacturer’s instructions (Table S3) ...
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bioRxiv - Molecular Biology 2023Quote: We used the proteome profiler human apoptosis array kit from R&D Systems (Mineapolis, MB, USA, Cat. ARY009) to assess apoptosis and apoptosis-related proteins according to manufactures instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... we pre-treated 1 mL of FBS with 2.5 mg of recombinant human ALK1-Fc (R&D systems) and rocked at room temperature for 2 hours before adding to 49 mL EGM2 for a final concentration of 50 µg/mL ALK1-Fc ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human ACE2 protein levels were quantified from lung homogenates by ELISA (R&D Systems #DY3437, #DY933).
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bioRxiv - Immunology 2024Quote: ... following reagents were used in flow cytometry: recombinant human PD-L1 Fc chimera Biotin protein (R&D Systems); APC Streptavidin (Tonbo Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... medium was changed every day to HDM with 20 ng/mL human recombinant BMP-4 (R&D Systems) and 20 ng/mL human recombinant FGF-4 (R&D Systems) ...
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bioRxiv - Cancer Biology 2024Quote: ... ELISA for HO-1 was performed using a human HO-1 DuoSet IC kit from R&D Systems, and total protein was assessed using a PierceTM BCA Protein Assay Kit.
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bioRxiv - Systems Biology 2020Quote: ... Primary detection of hACE-2 was done using a goat anti-hACE-2 antibody (1:1,250; #AF933, R&D Systems), a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5,000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then incubated in permeabilization buffer containing the primary REG3β antibody (1:250 dilution; R&D systems, Minneapolis, MN) overnight (16 h) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with a mixture of primary goat anti-ACE2 at 1/500 dilution (R&D Systems, #AF3437) and rabbit anti-GnRH (gonadotropin releasing hormone ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used overnight in 1% skimmed milk solution: anti-UCP1 (1:750, R&D Systems, MAB6158), anti-pCREB (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... pH6.0 at 95°C followed by 1h blocking and incubation with pre-optimized primary anti-UCHL1 (MAB6007, R&D Systems) or anti-p53 (Dako ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody information is as following: Goat anti-mouse aminopeptidase N/ANPEP (CD13; R&D systems; AF2335; 1:100), Rat anti-mouse vascular adhesion molecule (VCAM1 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and dilutions used for immunohistochemistry in this study were the following: PDGFRA (goat, 1:70; R&D Systems); GPNMB (goat ...
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bioRxiv - Physiology 2020Quote: ... followed by overnight incubation at 4 °C with primary antibodies against UCP1 (1:1000, R&D systems, Minneapolis, MN, USA), 14-3-3ζ (1:1000 Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were applied overnight at 4 °C: goat anti-OTX2 (1:500, R&D Systems, cat# AF1979), mouse anti-CDX2 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated with the appropriate primary antibody overnight at 4°C: anti-IL1 (goat, 1:1000, R&D Systems), anti-phosphorylated tau (AT8 ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence staining: goat anti-mouse VE-cadherin (R&D Systems, AF1002; 1:200), hamster anti-mouse PDPN (Developmental Studies Hybridoma Bank ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were incubated overnight at 4°C with the primary antibodies for A2B5 (1:50; R&D Systems, MAB1416) and NG2 (1:300 ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... antigen-retrieved in pH6 sodium citrate buffer and stain with primary antibodies including: anti-LYVE-1 (AF2089; R&D System), anti-CXCL8 (MAB208 ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs were incorporated into a 3D matrix made of rat tail type 1 collagen (R&D Systems) as previously described 95 ...
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bioRxiv - Molecular Biology 2020Quote: ... FGF-2 in the CM was assayed using a human FGF basic DuoSet ELISA Development Kit (R&D Systems) according to the manufacturer’s procedure.
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bioRxiv - Neuroscience 2019Quote: ... Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution) using DuoSet ELISA kits (R&D Systems). DuoSet ELISA Ancillary Reagent Kit 2 was used in conjunction with all DuoSet ELISA kits ...
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bioRxiv - Immunology 2019Quote: ... and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems, Ref. HS600C Lot P178994). All these immunoassays were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... TNF-α was measured using a Quantikine HS ELISA Human TNF-α (R&D Systems, Ref. HSTA00E Lot P174731) and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... CXCL8 and Osteopontin (SPP1) proteins in conditioned media was performed using a custom human luminex assay (R&D systems), according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: Phosphorylation status and total amounts of 43 kinases were measured using Human Phospho-Kinase Array from R&D Systems. 1.5×106 LN229 cells were stimulated with 400 ng/mL of GAS6 for 10 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... with 10% FBS (Wisent) and 0.5 ng/ml recombinant human Macrophage Colony Stimulating factor (M-CSF) (R&D Systems) to allow for differentiation of monocytes into macrophages ...
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bioRxiv - Bioengineering 2020Quote: ... 97 µL of urine was incubated with 1 µL of human cathepsin B (R&D Systems, 953-CY-010) and 1 µL of DTT for 15 minutes to inactivate the urine cysteine protease inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Physiology 2021Quote: ... The medium was supplemented with 100 ng/mL recombinant human M-CSF using CellXVivo™ kit (R&D Systems) and differentiated for 5–7 days prior to use.
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against human DDR1 (R&D Systems, AF2396), CXCL5 (Abcam ...
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bioRxiv - Immunology 2022Quote: ... For the IL-8 neutralization experiments: 0.4 μg/ml of human IL-8 monoclonal antibody (R&D Systems, USA) was added to the apical and basolateral compartment of enteroid monolayers and to the PMN suspensions before they were co-cultured ...
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bioRxiv - Microbiology 2022Quote: ... IFN-β amounts in the culture supernatant were measured by human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Immunology 2020Quote: ... and Alexa Fluor 594 labelled anti-human DAP10/HCST (R&D Systems, 1:500 v/v in blocking buffer) to stain for the cytoskeleton and DAP10 clusters respectively ...
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bioRxiv - Physiology 2021Quote: ... The membranes were incubated with a sheep polyclonal antibody to human DG (AF6868; R&D Systems, 1:100 dilution) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6 ...
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bioRxiv - Biochemistry 2021Quote: For TCR-mediated adhesion 96-well plates were pre-coated with 0.5μg human ICAM-1/well (R&D systems). Transfected cells were left untreated or stimulated with anti-TCR antibody (OKT3 ...
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bioRxiv - Physiology 2022Quote: ... Aβ ELISA was performed using a human amyloid β (aa1-42) immunoassay Quantikine ELISA kit (R&D Systems, Inc.).
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bioRxiv - Molecular Biology 2022Quote: ... human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF, 50 ng/mL R&D Systems, 7954-GM-020/CF), and interleukin-4 (IL-4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of GDF15 was measured using an ELISA assay for Human GDF-15 DuoSet (R&D systems, # DY957) as per the manufacturer’s instructions on a Tecan INFINITE® 200 Pro spectrophotometer ...
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bioRxiv - Pathology 2020Quote: ... the membranes were incubated with the sheep anti-human collagen I alpha 1 antibody (1:2000, R&D systems) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was titered using a commercial enzyme-linked immunosorbent assay kit (Human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml−1 ...
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bioRxiv - Immunology 2020Quote: ... The level of human or mouse CXCL10/IP-10 was determined using a DuoSet ELISA kit (R&D Systems) and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...