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Citations for R&D Systems :
1701 - 1750 of 2276 citations for Cow Hepatoma Derived Growth Factor HDGF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived-dendritic cells (moDC) were obtained by incubation of monocytes with 1 ng/mL of granulocytes-macrophages colony-stimulating factor (GM-CSF, R&D Systems, France) and Interleukin-4 (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were cultured in RPIM 1640 medium containing 20% porcine serum and 10 ng/mL recombinant porcine granulocyte-macrophage colony-stimulating factor (GM-CSF, R&D Systems, UK) to differentiate mature bone marrow-derived macrophage (BMDMs ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Pathology 2024Quote: ... the BMMs are incubated for three days in a 24-well plate with medium supplemented with 30 ng/ml M-CSF and 50 ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D Systems, MN). After this period ...
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bioRxiv - Cell Biology 2021Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...
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bioRxiv - Cancer Biology 2020Quote: ... Macrophage differentiation from bone marrow cells was obtained by culture in standard growth medium supplemented with m-CSF (R&D Systems) at 30 ng/ml for 7 days.
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Systems Biology 2019Quote: ... CCL2 and CCL5 secreted protein levels in cell supernatants from 1×106 murine BMDM’s was performed by ELISA (R&D Systems) according to manufactureŕs instructions.
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bioRxiv - Bioengineering 2021Quote: ... Serum IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Cancer Biology 2020Quote: Concentrations of murine IL-11 in the culture supernatants were determined by ELISA according to the manufacturers’ instruction (R&D Systems).
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Human soluble E-selectin levels were assayed using quantitative sandwich enzyme immunoassay technique and following the manufacturer’s protocol (Quantikine ELISA Human E-selectin/CD62E Immunoassay, DSLE00, R&D Systems). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were later determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Bioengineering 2021Quote: ... Total TGF-β1 levels were assessed using Human TGF-beta 1 DuoSet® ELISA (DY240; R&D Systems™, Minneapolis, MN). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... IFNβ and IFNλ protein levels from HCT-8 cultures were measured by Human IFN-beta DuoSet ELISA (DY814, R&D Systems) and Human IL29/IL28B (IFN-lambda 1/3 ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IFNλ from intestinal biopsies were measured by Mouse IL28B/ IFN-lambda 3 DuoSet ELISA (DY1789B, R&D Systems). Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Lambda interferon was evaluated by Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Interleukin-6 was measured by Human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... Interleukin-6 was measured in duplicate using a commercially available high-sensitive enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis) with a minimum detection threshold of 0.2 pg/ml ...
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bioRxiv - Immunology 2021Quote: ... In-Cell ELISA was then developed using DY999 substrate solution according to the manufacturer’s recommendations (R&D Systems, Minneapolis, MN USA) and measured at 450 nm (and at 630 nm to assess the background ...
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bioRxiv - Immunology 2020Quote: ... The cytokine concentrations in the culture supernatant were determined using quantitative ELISA (Mouse TNF and IL-10 DuoSet; R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... IL-1β levels were measured via a mouse IL-1β ELISA assay according to the manufacturer’s instructions (R&D Systems, MLB00C). Released IL-1β levels were normalized to cellular protein ...
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bioRxiv - Physiology 2020Quote: Fecal lipocalin-2 levels in distal colon fecal contents were analyzed using Mouse Lipocalin-2 DuoSet ELISA (R&D Systems, DY1857) according to manufacturer provided instructions ...
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bioRxiv - Microbiology 2019Quote: ... and lipocalin-2 (Lcn-2) protein was assayed in Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA). At the end of DSS treatment ...
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bioRxiv - Microbiology 2020Quote: ... Murine KC and JE concentrations were determined in the lung homogenate supernatants by DuoSet® ELISA Development Systems (R&D Systems), according to manufacturer instructions.
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bioRxiv - Cell Biology 2019Quote: ... Luminex® Multiplex and ELISA assays for the detection of chemokines and cytokines were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: IL-10 and TNFα in serum and CM-MSC were quantified using mouse Quantikine ELISA IL-10 immunoassay (R&D Systems) and TNFα ELISA high sensitivity (eBioscience ...
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bioRxiv - Genetics 2022Quote: Supernatants from transfected HEK293T cells were analyzed for their LBP content by Human LBP DuoSet ELISA (#DY870-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Plasma and urine GDF15 were measured by ELISA test (MG150 and DGD150, for rodent and human GDF15, respectively, R&D Systems) and tissue GDF15 was measured using an ELISA test (Elabscience).
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants are collected and frozen at -20°C for cytokine assays performed by ELISA according to manufacturer’s instructions (DuoSet, R&D Systems).
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bioRxiv - Molecular Biology 2023Quote: ... TGFβ concentration in the cell free BALF was calculated using Mouse TGFβ 1 DuoSet Elisa (R&D Systems Cat DY1679-05) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Harvested CM was normalized to the fibroblast cell count and assessed for IGFBP2 using ELISA purchased from R&D system (DGB200). Serum samples were diluted 50-fold ...
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bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
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bioRxiv - Immunology 2023Quote: ... Just prior to analysis plasma samples were thawed and then assayed for numerous analytes (using dilutions optimized in prior studies) according to the manufacturer’s guidelines: adiponectin using the adiponectin/Acrp30 ELISA (DY1065, R&D System, lower limit of detection [LLoD] 15.6pg/ml) ...
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bioRxiv - Physiology 2024Quote: ... supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100). BCA assay was performed to normalize IGF-1 concentration to protein concentration in the liver ...
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bioRxiv - Neuroscience 2020Quote: ... L-glutamine and 10 ng/ml of carrier-free murine recombinant granulocyte and macrophage colony-stimulating factor (GM-CSF, 415-ML, R&D systems, Minneapolis, MN). Cell suspension from one brain was plated in a T75-cell culture flask coated with poly-D-lysine (P7280 ...
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bioRxiv - Immunology 2021Quote: ... the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF; 1000 U/mL; R&D Systems, Minneapolis, MN) and interleukin 4 (IL-4 ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Developmental Biology 2019Quote: ... The final concentration of Rspondins in the culture medium was initially tested in pilot experiments in order to add comparable amounts of bioactive ligands (bioactivity measured by TOPflash assays and organoid growth curves, R&D systems). Culture medium was changed each other day and after 5-6 days in culture ...
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bioRxiv - Developmental Biology 2020Quote: All OPCs were grown on PO and laminin coated flasks in growth media consisting of DMEM/F12 supplemented with N2 Max (R&D Systems), B27 (Thermo Fisher) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...