Labshake search
Citations for R&D Systems :
1651 - 1700 of 2276 citations for Cow Hepatoma Derived Growth Factor HDGF ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels for IL-6 and MCP-1 were determined by ELISA according to manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were added to the culture plates at the density of 2.0 x 106 cells / dish in a final volume of 10 mL of complete RPMI-1640 supplemented with 20 ng/mL GM-CSF (granulocyte-macrophage colony stimulating factor; R&D Systems). After 3 days ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Immunology 2024Quote: ... the medium was enriched with recombinant murine interleukin-3 (IL-3; 5 ng/mL) and stem cell factor (SCF; 5 ng/mL) from R&D Systems, Minneapolis ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Cell Biology 2023Quote: ... A feeding layer of 2 ml RPMI growth medium supplemented with 10 ng/ml PDGF-BB ligand (R&D Systems) and/or 0.01 uM siRNA (see below ...
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bioRxiv - Genomics 2020Quote: ... IL-13 and IFN-γ in the supernatant (Quantikine ELISA D5000B, D1300B and DIF-50, R&D Systems, Minneapolis, MN). Specifically ...
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bioRxiv - Immunology 2019Quote: ... in which plasma levels of soluble CD14 (sCD14) were determined by ELISA-sCD14 Quantikine assay (R&D Systems Minneapolis, MN) according to the manufacturer’s protocol and surface expression of combined HLA-DR and CD38 on CD4+ and CD8+ T cells was analyzed by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
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bioRxiv - Bioengineering 2020Quote: ... IL-15 superagonist was quantified using the human IL-15/IL-15R alpha complex DuoSet ELISA (R&D Systems DY6924). For BiTE experiments with primary human T cells ...
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bioRxiv - Neuroscience 2020Quote: ... crassiceps excretory/secretory (E/S) product using commercially available ELISA reagents according to the manufacturer’s instructions (R&D Systems, Germany). 96 well plates were coated with 50 μl antibody solution (IL-1β ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
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bioRxiv - Biochemistry 2021Quote: ... TNFα and IL-6 in supernatants were measured using ELISAs (Mouse TNFα; Mouse CCL5; Mouse IL-6, R&D Systems) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... the conditioned medium was collected and TNF-α was quantified by enzyme-linked immunosorbent (ELISA) assay from R&D Systems according to the instructions of the manufacturer.
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bioRxiv - Immunology 2022Quote: ... PTX3 and P-selectin were determined by enzyme-linked immunosorbent assay (DuoSet ELISA, R&D Systems and Cloud-Clone corp) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Physiology 2020Quote: ... IL-33 in colonic mucosa of distal colon was analyzed using Mouse IL-33 DuoSet ELISA (R&D Systems, DY3626) according to manufacturer instructions.
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bioRxiv - Cell Biology 2019Quote: Cytokine (IL6 and CXCL8) levels in tissue culture supernatants were determined by ELISA assay (DY206 and DY208; R&D Systems). All assays were performed according to the manufacturer’s instructions and read on a CLARIOstar microplate reader (BMG Labtech) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... The DuoSet ELISA systems were used according to the manufacturer’s instructions (DY201, DY206, DY210, DY1607, DY217B, R&D Systems, respectively). Absorbance values were measured on Infinite® 200 Pro plate reader (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ELISAs were used to quantify CTSB and CTSS levels in CSF (both R&D DuoSets, R&D Systems, Minneapolis, USA). CSF samples were diluted 20x with reagent diluent prior to ELISA analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Concentration of PCSK9 in the plasma and tissue samples were assessed by ELISA (Quantikine MPC900, R&D Systems, Minneapolis, MN). Protein extracts were obtained after lysis of tissues in 350-μL Tris HCl buffer pH=9.5 (10 mM ...
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bioRxiv - Immunology 2024Quote: The chemokine CXCL13 was measured in LO chip effluent using a human CXCL13 ELISA assay (Quantikine, R&D Systems #DY801), following the manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: Levels of active TGF-β1 in triceps were quantified using Quantikine ELISA mouse TGF-β1 immunoassay (R&D Systems, MB100B) according to the manufacturer’s recommendations and as previously described24 ...
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bioRxiv - Immunology 2023Quote: Serum IL-6R levels were measured using an immunoassay method validated at QPS (Quantikine ELISA; R&D Systems Minneapolis, MN). Serum IL-6 and CRP levels were measured using in vitro diagnostic methods validated at PPD (Roche Cobas ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2023Quote: ... The supernatant was analyzed using the mouse Lipocalin-2 enzyme-linked immunosorbent assay (ELISA) Duoset (DY1857-05, R&D Systems) according to the manufacturer’s instruction in serial dilutions.
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were collected 24 h later and analyzed for IL-17A by DuoSet ELISA (R&D Systems, Minneapolis, USA). Concentrations of IL-17A in the used CM was 764 pg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioned media was collected from supernatant at first passage and assayed with the Mouse VEGF DuoSet ELISA (R&D Systems) according to manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: The levels of secreted IFN-β were measured by an IFN-β enzyme-linked immunosorbent assay (ELISA) (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
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bioRxiv - Microbiology 2021Quote: ... the BMMs became adherent for the osteoclastic differentiation using a complete DMEM supplemented with 50 ng/ml of receptor activator of nuclear factor kappa-B ligand (RANKL, R&D System, USA) and 20 ng/ml of M-CSF (R&D System ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...