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Citations for R&D Systems :
1601 - 1650 of 1736 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: All cells were grown in a humidified incubator at 37°C and 5% CO2 and cell lines tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems).
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bioRxiv - Cancer Biology 2020Quote: ... Then we collected the supernatants and cell lysates so that we could detect the chemokines using a Proteome Profiler Human Chemokine Array Kit (R&D Systems, ARY017).
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bioRxiv - Cell Biology 2020Quote: ... papillary or reticular cell sheets were seeded with HUVECs at 6 000 cells /cm2 in ECGM2ΔFGFΔVEGF supplemented or not with VEGF (R&D systems, Minneapolis MN, USA) at 5 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
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bioRxiv - Biochemistry 2021Quote: Acvr1[R206H]/+and Acvr2a −/−;Acvr2b −/−;Bmpr2 −/−;Acvr1[R206H]/+ mES cells were dissociated using non-enzymatic cell dissociation buffer (MiliPore, S-004-B) and resuspended in the flow cytometry staining buffer (R&D systems, FC001). After 15 minutes of blocking (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
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bioRxiv - Pathology 2020Quote: Osteoprotegerin was measured in culture supernatants of cells and slices using a murine OPG DuoSet® ELISA kit (R&D Systems, Minneapolis, US) according to the instructions provided by the manufacturer.
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested after treatment and stained for PD-L1 (eBioscience, 12-5983-42) and MHC class I (R&D Systems, FAB7098G). Induction of the respective proteins was analyzed by flow cytometry.
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bioRxiv - Neuroscience 2021Quote: ... the astrocyte-enriched cell suspension was incubated for 20 minutes at room temperature on a dish coated with anti-HepaCAM antibody (R&D Systems MAB4108) to bind astrocytes ...
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Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)bioRxiv - Biochemistry 2021Quote: ... We collected the cell culture supernatant and measured the TNF-α concentration using Mouse TNF-α Quantikine ELISA Kit MTA00B (R&D SYSTEMS).
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bioRxiv - Microbiology 2021Quote: ... One million cells were re-suspend in 100 μl PBS and either immediately incubated with 0.25 μg anti-ACE2 antibody (R&D systems, Cat.# AF933) or first incubated at 37 °C for 6 hours with shaking to allow recovery of cell surface proteins after trypsinization ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...
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bioRxiv - Genomics 2021Quote: ... a total of ∼200 million cells were harvested and stained for surface ACE2 abundance as previously described with ACE2 antibody (R&D Systems #MAB9332) at 1:50 dilution in FACS buffer (PBS supplemented with 2% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNγ released into the supernatant by CAR-T cells was then measured using the DuoSet ELISA kit for mouse IFNγ (R&D systems, DY485) and Nunc MaxiSorp flat bottom plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
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bioRxiv - Immunology 2024Quote: IFN-γ protein levels were measured from cryopreserved neuron-CD8+ T cell co-culture supernatant using the Mouse IFN-γ DuoSet ELISA kit from R&D Systems (R&D Systems ...
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bioRxiv - Immunology 2023Quote: IFN-γ secreted in the supernatant after 72h of co-culture of tumor and effector T cells was measured using the Human IFN-gamma DuoSet ELISA kit (R&D Systems, DY285B) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:500, rabbit anti-PDGFRα (3164 Cell Signalling Technologies, Danvers, Massachusetts) 1:500 and goat anti-Sox10 (AF2864 R&D Systems, Minneapolis, Minnesota) 1:50 ...
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... A time course was performed on cells treated with 300 ng/mL of recombinant mouse VEGF-B186 (R&D Systems #767-VE-010) for 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Levels of vascular endothelial growth factor (VEGF) were evaluated by sandwich enzyme-linked immunosorbent assay (ELISA) in GL261 cell supernatants using matched antibody pairs from R&D Systems (USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Generated hiPSC lines were layered onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Genetics 2023Quote: Fully differentiated Day 30 Cardiomyocytes were resuspended onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
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bioRxiv - Cancer Biology 2023Quote: The receptor tyrosine kinase (RTK) activities in cells after drug treatment were analyzed by a Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, ARY001B) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: IFN-γ and granzyme B concentrations in the supernatants of CD8+ T cells were measured using a mouse ELISA kit (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... HUES8 cells were treated with 3μM CHIR99021 (CHIR, Stem-RD, Hoover, AL) and 100 ng/mL Activin A (AA, R&D systems, Minneapolis, MN) in RPMI media (Cellgro ...
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bioRxiv - Genomics 2023Quote: ... were assessed from the culture supernatant of the activated CD4+ T cells by Human Th9/Th17/Th22 Luminex Performance Assay 17-plex Fixed Panel (R&D systems, USA). The assay was performed according to manufacturers instructions and samples diluted 1:2 with calibrator diluent ...
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bioRxiv - Immunology 2023Quote: ... T cells were analyzed using ELISpot according to the manufacturer’s protocol ELISpot Rat interferon-gamma (IFNγ) (Cat: EL585 by R&D Systems, United States).
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bioRxiv - Genetics 2023Quote: ... and colonies were picked into a Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 24-well plate for expansion ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Immunology 2023Quote: The two cytokines IFNγ and TNFα were detected from cell culture supernatants of the degranulation assays utilizing the respective R&D DuoSet (R&D Systems, USA) ELISAs per manufacturers’ recommendation.
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with an equal volume of 1 mg/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2h ...
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bioRxiv - Cancer Biology 2024Quote: ... mice (6-8 weeks old) were injected with 1x106 cells suspended in PBS and Cultrex™ basement membrane extracts (BME) (Bio-techne/R&D Systems) into the right flank ...
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bioRxiv - Developmental Biology 2020Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
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bioRxiv - Bioengineering 2021Quote: Conditioned media from each cell-line/passage (at the time of cell harvest) were collected and prepared for cytokine profiling using the Human Premixed Multi-Analyte Magnetic Luminex Assay (R&D Systems, Minnneapolis MN) (Supplemental Table 3) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell lines were purchased from ATCC (Manassas, VA, USA) and confirmed to be mycoplasma free by MycoProbe kit (R&D Systems, Minneapolis, MN, USA). Cells were grown at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: Twenty thousand HUVEC cells were seeded in reduced growth factor basement membrane extract (BME)-coated 96-well plate (R&D systems: 3470-096-K). Endothelial cells were exposed to 100 μl of the different benign or malignant cells conditioned media ...