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Citations for R&D Systems :
1501 - 1550 of 1736 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Molecular Biology 2021Quote: ... washed twice with 1x PBS and stained using the H/MM pluripotent Stem Cell Multi-Color Flow Cytometry kit (R&D Systems) according to vendor’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cell suspensions of wild type and Pds5a KO mESCs were permeabilized and fixed using the fixation/permabilization buffer (R&D systems), washed twice with 1x PBS and stained using the H/MM pluripotent Stem Cell Multi-Color Flow Cytometry kit (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... while 29-13 cells were passaged in Beck’s Medium supplemented with 15% doxycycline-free fetal calf serum (R&D Systems—Minneapolis, MN), 50 μg mL-1 hygromycin (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... microglial cells were preincubated with 20µg/ml of anti-C3aR antibody (Catalog # MAB10417) or control IgG (R&D Systems, Inc. Minneapolis, MN) suspended in DMEM/F12 supplemented with 5% heat-inactivated FBS ...
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bioRxiv - Immunology 2022Quote: ... The cells were again divided and incubated in the presence or absence of 50 ng/ml TGFβ (R&D Systems, Minneapolis, MN). Cells were harvested at 30 minutes and 24 hours post addition of TGFβ and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: CD4+ splenic T-lymphocytes were polarized to TH17 ex vivo by use of CellXVivo Mouse Th17 Cell Differentiation Kit (#CDK017, R&D Systems). Briefly ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatant from treated cells were collected and applied to the IP-10 antibody-coated plate for ELISA analysis (R&D Systems) following the manufacture’s instruction.
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bioRxiv - Cancer Biology 2022Quote: ... six- to eight-week old NSG mice were subcutaneously injected into the flank with 2×106 wildtype or pre-transduced A-673 or RD-ES EwS cells in Cultrex Basement Membrane Extract (R&D Systems) to enhance tumor formation ...
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bioRxiv - Cancer Biology 2020Quote: ... Soluble LAG3 was also evaluated by ELISA in bulk and sorted peripheral CD8+ and CD4+ T cells ex vivo utilizing a sLAG3 ELISA kit (R&D Systems). A 96 well plate was coated with a capture antibody for 24 hours followed by sample and standard incubation ...
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bioRxiv - Immunology 2020Quote: Levels of human IL-1β (DY201) and IL-18 (DY318) were measured in the cell supernatants using ELISA kits from R&D Systems. Human IL-6 (# 88-7066-86 ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Intracellular granzyme B and INFγ staining was performed in cells that had fixed and permeabilized in Flow Cytometry Fixation and Permeabilization Buffers (Catalog numbers FC004, FC005, R&D systems) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Immunology 2020Quote: Intestinal tissues from mice treated with PBS or spore for 7 d and 45 d were lysed with cell lysis buffer (R&D Systems) supplemented with 1% 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2019Quote: Protein levels in cell-free sputum samples of CXCL10/IP-10 and CCL5/RANTES were measured using duoset ELISA kits (R&D Systems), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... and Fc-ICAM-1 or Fc-VCAM-1 (Intercellular Adhesion Molecule 1; Vascular Cell Adhesion Molecule 1, R&D system, Minneapolis, MN) solution at 10 μg/mL overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Cell supernatants were collected and levels of IFNλ were determined with the IFN-lambda 1/3 DuoSet ELISA kit (R&D Systems). Plates were read on a Synergy HT plate reader (Biotek ...
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bioRxiv - Neuroscience 2019Quote: ... and cells were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4 ...
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants were collected and IL-8 secretion was determined using IL-8 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of TGFβ secreted by mouse pancreatic tumor cells was quantified by using the Mouse TGFβ 1 DuoSet ELISA (#DY1679-05, R&D systems), complemented with Sample Activation Kit 1 (#DY010 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 5 × 105 cells in 100 μl adhesion buffer were added to the upper chamber and either 500 ng/ml CCL2 (R&D Systems) or 10 nM C5a (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Immunology 2019Quote: ... normal T cell expressed and secreted (RANTES) and tumor necrosis factor alpha (TNF-α) by ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Cell Biology 2019Quote: ... Cxcl10 and Ccl22 in mouse peritoneal cells were analyzed by ELISA according to the manufacturers instructions (Human and Mouse DuoSet ELISA respectively, R&D Systems). For relative chemokine concentration in comparison to parasite load ...
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bioRxiv - Developmental Biology 2019Quote: ... or β-CATENIN cells were grown in Basal medium in 60 mm dishes and treated with either 50 ng/ml ActA (R&D systems) or with WNT3A/L-cell conditioned medium (L-CM)11 for 6h ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated for 24 hours either with vehicle or with 10nM GnRH and 1nM activin A (R&D Systems, Minneapolis, MN) in serum-free DMEM with 0.1% BSA.
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bioRxiv - Cell Biology 2020Quote: ... and chondrogenic differentiation experiments were performed following the instructions of the human mesenchymal stem cell functional identification kit (R&D systems, SC006). For osteogenic differentiation ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were exposed to 1 µM EdU along with or withpout 2-100 ng/ml recombinant VEGFA (R&D Systems, 493-MV) for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with either M1-activation medium [RPMI160 medium with 20 ng/ml IFNγ (485-MI-100; R&D systems) and 10 ng/ml LPS (L2630 ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Developmental Biology 2020Quote: ... a brief wash with DMEM/F12 is followed with Day0 medium 30ng/ml Activin A (Cell Guidance Systems) + 40ng/ml BMP4 (R&D Systems) + 6uM CHIR99021 (Tocris ...
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 cells expressing dox-inducible shNT or shUGUUUGC shRNAs were mixed with 50% basement membrane extract (R&D Systems, Minneapolis, MN, USA) and injected subcutaneously into the flanks (3×106 cell per injection) ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Immunology 2022Quote: ... CXCL10 was measured in plasma and cell supernatant using a DuoSet ELISA Kit (R&D Systems DY466-05, R&D Systems DY008B). ELISAs were performed according to manufacturer’s protocol.