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Citations for R&D Systems :
1501 - 1539 of 1539 citations for Mouse ONECUT3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Cell Biology 2022Quote: Intracellular cAMP expression levels were measured after 10min to 48h of PTH treatment using Mouse/Rat cAMP Assay Parameter™ Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions in both pre-differentiation COBs and BMSCs ...
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bioRxiv - Neuroscience 2024Quote: ... 10-week-old control female and 13-weekold control male mice (Esr1fl/fl) were injected daily with recombinant mouse NOV/CCN3 at 7.48 μg/kg (R&D Systems Catalog # 1976-NV-050) or vehicle control (0.9% NS) ...
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bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were analyzed by SDS-PAGE and immunoblot using an anti-histidine tag antibody (Monoclonal Mouse IgG1 histidine tag antibody; R&D Systems, cat. no. MAB050). Fractions containing TDO2 were combined and dialyzed (molecular weight cutoff 3.5 kDa ...
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bioRxiv - Neuroscience 2022Quote: ... TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: The cytokine/chemokine profile of mice plasma or culture supernatants was determined by using proteome profiler mouse cytokine array kit (R&D Systems, CAT. No. ARY006). The expression levels of cytokine/chemokine were measured by pixel density of each dot using the ImageJ software ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Molecular Biology 2023Quote: ... The cytochrome c concentrations in the isolated cytosolic fraction were measured by rat/mouse cytochrome c quantikine ELISA Kit (MCTC0, R&D Systems, Inc., Minneapolis, MN).
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels within the lung and brain homogenates of infected mice were analyzed using the Proteome Profiler Mouse XL Cytokine Array (R&D Systems Inc., Minneapolis, MN). The relative expression levels of 111 soluble mouse proteins were determined following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ splenic T-lymphocytes were polarized to IL-17A-producing phenotypes ex vivo by use of CellXVivo Mouse TH17 Cell Differentiation Kit (#CDK017, R&D Systems, Minneapolis, MN, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... the IGF-1 concentration in the medium was measured using the Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA)
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... 1.5 mL of medium from each condition was collected for cytokine analysis and relative intensity of a panel of cytokines was measured (R&D Systems, Mouse Cytokine Array Kit).
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Immunology 2022Quote: Extracellular calprotectin was measured in plasma and culture media using the Mouse S100a8/S100a9 Heterodimer DuoSet Solid Phase Sandwich ELISA Kit (R&D Systems #DY859605, Minneapolis, MN, USA) as per manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... #CCL-131) were plated in each well of 96-well plates and preactivated with 100 ng/mL mouse IFN-γ (R&D Systems, #485-MI-100) for 18-24 hours ...
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Genetics 2024Quote: The concentration of IGF-1 in the liver was quantified using the Mouse/Rat IGF-I/IGF1 Quantikine ELISA Kit (MG100, R&D Systems, Inc., Minneapolis, MN, USA). Liver protein lysates were diluted at a ratio of 1:6 in calibrator diluent and the assay was performed following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: Wild type C57BL/6 10-week-old female mice were treated daily with recombinant tumor necrosis factor-like weak inducer of apoptosis (TWEAK 1237-TW; R&D Systems; 2 ug per mouse) for 4 days followed by a single i.p ...
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bioRxiv - Immunology 2021Quote: ... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
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bioRxiv - Developmental Biology 2021Quote: ... From each litter we used cells at passage one for treatment with 50 ng/ml mouse recombinant IGF2 (R&D Systems, 792-MG-050; dissolved in PBS), 1000 ng/ml human IGF2Leu27 (GroPep – TU100 ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
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bioRxiv - Immunology 2023Quote: ... the pellets obtained after centrifugation of the BAL fluid were pooled and stained for 30 min at 4°C with unconjugated polyclonal goat anti-mouse Ym1/2 (AF2446, R&D Systems; 1:100 diluted in PBS). After washing ...
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bioRxiv - Immunology 2020Quote: ... Immature BMDCs were harvested from femurs and tibias of 8-to12-week-old mice and cultured in the presence of recombinant mouse GM-CSF (R&D Systems cat. 415-ML, 20 ng/ml) and IL-4 (R&D Systems cat ...
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bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...