Labshake search
Citations for R&D Systems :
1451 - 1500 of 1539 citations for Mouse ONECUT3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Immunology 2021Quote: ... 2×105 isolated TILs have been cultured in vitro in 96well plate in presence of 20ng/ml mouse IL-2 (R&D System 402-ML), 20ng/ml mouse IL-7 (R&D System 407-ML ...
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bioRxiv - Cell Biology 2021Quote: ... and diluted to equal concentrations before performing an enzyme-linked immunoabsorbent assay (ELISA) using a Mouse/Rat FGF21 Quantikine ELISA kit (R&D Systems, Minneapolis, MN)(57).
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bioRxiv - Microbiology 2021Quote: Virus-inactivated serum samples or lung supernatants from SARS-CoV-2 VoC infected mice were added to a custom 8-plex Mouse Magnetic Luminex Assay (R&D Systems LXSAMSM-08) including IL-6 ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with fresh media (DMEM + 10% FBS) supplemented with 0.03 ng ml−1 of recombinant mouse IFNγ (R&D Systems, #485-MI-100). After 24 h treatment ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of IP10 in the media was then determined by ELISA using the Mouse CXCL10/IP-10/CRG2 DuoSet ELISA from R&D system (cat. DY466) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... or Recombinant Mouse Wnt-3a (10µg/ml stock solution in 0.1% BSA in PBS) (1324-WN-002, R&D systems, Bio-Techne, Minneapolis, MN) with DMSO and 0.1% BSA in PBS as vehicle controls ...
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bioRxiv - Immunology 2022Quote: ... the blood was centrifuged with 2000g for 20min and the supernatant (serum) was collected for TPO measurement using Quantikine Mouse Thrombopoietin ELISA Kit (R&D Systems, Minneapolis, USA) to measure serum TPO levels ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein was applied to each ELISA membrane and developed according to the manufacturer’s instructions (Proteome Profiler Mouse XL Cytokine Kit, ARY028, R&D Systems, Minneapolis, MN, USA). Cytokine array signals were detected by X-ray film ...
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bioRxiv - Systems Biology 2021Quote: ... we added one or combinations of the following recombinant proteins to the medium: 200 ng/mL of recombinant mouse FGF4 (R&D Systems, #7486-F4), 200 ng/mL of recombinant human FGF5 (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Immunology 2021Quote: ... the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF; 1000 U/mL; R&D Systems, Minneapolis, MN) and interleukin 4 (IL-4 ...
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bioRxiv - Immunology 2021Quote: Concentration of Activin A in human and mouse serum samples were performed using sandwich ELISA-Activin A immunoassay kit from R&D system (Cat DAC008).
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bioRxiv - Immunology 2019Quote: ... and the tissue levels of myeloperoxidase (MPO) in the supernatants were determined using a mouse MPO ELISA (R&D systems, Biotechne, Minneapolis, MN, USA). The fixed tissues (left lungs ...
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bioRxiv - Cancer Biology 2020Quote: The relative content of 111 cytokine proteins (Table S1) was determined using the dot blot based mouse XL cytokine array kit following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Immunology 2021Quote: ... magnetically isolated T cells were resuspended in the complete medium and were seeded in a 10-cm petri dish or T-75 flask at 1–2×106 cells/cm2 and cultured in the presence of 20 ng/mL recombinant mouse interleukin-7 (rmIL-7; 402-ML-020/CF, R&D Systems, Minneapolis, MN) for 24 hr at 37°C in a humidified 5% CO2 atmosphere.
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bioRxiv - Immunology 2021Quote: ... Mouse CXCL2/MIP-2 DuoSet ELISA (Catalog no. DY452-05) and mouse CXCL1/KC DuoSet ELISA (Catalog no. DY453-05) were obtained from R&D Systems (Minneapolis, MN).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 were assayed using a Luminex mouse pre-mixed Multi-Analyte kit according to standard instructions (LXSARM-06, R&D System, Minnesota, USA).
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... the top ring (as viewed in Figure 2d) on each waveguide was spotted with mouse IgG1 isotype control antibody (R&D Systems, Minneapolis, MN) at 1000 μg/mL ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Evolutionary Biology 2023Quote: Lipocalin-2 concentration in the supernatants was measured using the commercial kit Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) for mouse Lipocalin-2 (DY1857) ...
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Physiology 2022Quote: ... Plasma ICAM-1 and VCAM-1 were measured using the Mouse ICAM-1/CD54 and VCAM-1/CD106 Quantikine ELISA kits according to the handbooks (R&D Systems, Minneapolis, USA).
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Pathology 2023Quote: ... These cytokines were analyzed using a cytokine array kit according to the manufacturer’s protocol (Proteome Profiler Mouse Cytokine Array Kit, Panel A; R&D Systems, Minneapolis, MN, USA). Plasma samples (100 μL/group ...
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bioRxiv - Neuroscience 2023Quote: ... Fractalkine protein levels were measured in hippocampal lysates via the R&D System’s Mouse Fractalkine (CX3CL1) Quantikine ELISA Kit (R&D Systems cat. Nr. MCX310) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5x diluted serum from end-stage HCC-bearing and 15-weeks control mice using the Proteome Profiler Mouse XL Cytokine Array (R&D systems, cat.no. ARY028) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4℃ with the following primary antibodies: anti-Nestin (rabbit, 1:500, Millpore, PN-MAB5326) and anti-ki67 (mouse, 1:500, R&D system, PN-AF7649); anti-CHI3L1 (rabbit ...
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bioRxiv - Genomics 2024Quote: FACS-sorted Vcam1-FAPs were grown to 60-80% confluence followed by adipogenic differentiation and were treated with 500 ng/mL recombinant mouse sFRP-1 protein (R&D systems, 9019-SF) with or without sFRP-1 inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... The secretion of IFN-γ was also quantified in cell supernatants using a sandwich mouse IFN-γ Duo Set ELISA (DY485-05, R&D systems). Standards and samples were diluted appropriately to obtain results within the stated detection levels.
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were subsequently washed with PBS without Ca2+ and Mg2+ and then incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Neuroscience 2021Quote: Serum FGF21 protein levels of FGF21-Tg and WT C57BL/6J littermates were determined using a mouse/rat solid-phase FGF21 Quantikine ELISA Kit MF2100 (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The levels of CXCL1 in BALF and TNFα in serum were determined using Quantikine® mouse ELISA kits (R&D systems, Inc., Minneapolis, MN, USA). The Candida β-glucan concentration in BALF or serum was determined with Glucatell kits (Associates of Cape Cod ...
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bioRxiv - Cancer Biology 2020Quote: ... Invasion was also quantified in response to serum-free DMEM supplemented with 10 ng/mL of recombinant mouse CSF2 (R&D Systems, 415-ML-5), PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems ...
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bioRxiv - Biochemistry 2022Quote: ... mice aged 6.5 weeks were starved for 6 hours and intraperitoneally injected with 220ng/g body weight of recombinant mouse FGF23 (2629-FG-025 R&D Systems, Minneapolis, MN, USA) in PBS-BSA 0.1% or PBS-BSA 0.1% vehicle with injection volume of 4μL/g body weight ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
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bioRxiv - Neuroscience 2019Quote: ... half of the culture media was replaced with media alone or media containing recombinant mouse netrin-1 (R&D systems cat. no.1109-N1/CF), recombinant human ephrin-A5-Fc (R&D systems cat ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...