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Citations for R&D Systems :
1351 - 1389 of 1389 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-SARS-CoV-2 spike S2 subunit mAb (IgG2a, Cat#: MAB10557) and mouse anti-SARS-CoV-2 nucleocapsid protein mAb (IgG2b, Cat#: MAB10474) were from R&D Systems (Minneapolis, MN). Mouse anti-SARS-CoV-2 membrane protein mAb E5A8A (IgG1 ...
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bioRxiv - Neuroscience 2024Quote: ... 50 mL of sample diluted 1:2-1:10 depending on the experiment or human TREM2-Fc standard (R&D Systems #1828-T2-050) were added to the plate and incubated at room temperature for one hour with shaking ...
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bioRxiv - Immunology 2020Quote: ... IL-15 and IL-18 (rhIL-12, R&D Systems, 5 ng/mL; rhIL-15, Pepro-Tech, 20 ng/mL; rhIL-18, R&D Systems, 50 ng/mL), or left unstimulated without further intervention ...
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2021Quote: ... After 48 hours the cells were fed with stage 2 differentiation medium consisting of STEMDiff APEL supplemented with 50 ng/mL of VEGF 165 (R&D Systems, 293-VE-010), 25 ng/mL of BMP4 (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 107 cells per sample were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and incubated with 50 µg/mL SARS-CoV-2 spike S1 subunit-Fc fusion protein (R&D systems, #10623-CV-100) or human IgG1 isotype control (BioXCell ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Ang-2 and MCP-1 were measured from individual samples following the manufacturer’s protocols of respective Quantikine ELISA Kits (R&D systems, DVE00, DANG20 and DCP00). Cytokine expression profiles of 2D cultures were assessed using a human angiogenesis antibody array (Abcam) ...
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bioRxiv - Physiology 2022Quote: ... and phospho-JNK (T183/Y185 for JNK1/2 and T221/Y223 for JNK3) with commercial kits (DuoSet IC ELISA, R&D Systems, # DYC1018B and # DYC1387B) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were resuspended in 1 part Advanced DMEM/F12 and 2 parts Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems; 3533-010-02) for a final solution of 66% Cultrex ...
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bioRxiv - Immunology 2022Quote: ... Fecal LCN2 levels in the supernatant fraction were estimated using a Duoset murine LCN-2 ELISA kit (R&D Systems DY1857-05, Minneapolis, MN, USA) based on provided directions.
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bioRxiv - Developmental Biology 2024Quote: ... washed gently three times with warm DMEM-F12 and refed with mTeSR without ROCKi for 2 hours followed with mTeSR containing 50ng/ml BMP4 (R&D systems, #314-BP-010) for 42-48 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... The hPSCs were plated at ∼4x104 cells/cm2 and allowed to grow for 2 days before changing with “Day 0 medium” consisting of the “base medium” plus 20 ng/mL BMP4 (R&D Systems 314-BP-050), 30 ng/mL Activin A (R&D Systems 338-AC-050) ...
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bioRxiv - Cancer Biology 2024Quote: ... or 5 days (SCLC cell lines) to allow for at least 2 cell doublings before processing in an MTT proliferation assay (R&D systems, #4890-050-K) performed according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... cell staining was done using the following conjugated antibodies and their IgG controls: Allophycocyanin (APC)-conjugated mouse IgG2a anti-human Neuropilin-2 (R&D systems, catalog no. FAB22151A) with allophycocyanin (APC)-conjugated mouse IgG2a control (R&D systems ...
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bioRxiv - Bioengineering 2024Quote: ... were transferred into Neural Induction Media (NIM) composed of DMEM/F-12 supplemented with 1x N-2 MAX Media Supplement (R&D Systems, Cat no. AR009), 1x GlutaMAX ...
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bioRxiv - Bioengineering 2024Quote: HLOs in BME-2 were either collected manually by pipetting in cold PBS-/- or isolated from BME-2 using Cultrex organoid harvesting solution (R&D Systems; 3700-100-01) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were incubated at room temperature for 2 hours with primary antibodies (Megalin/LRP2, MyBiosource MBS690201, 1:500) (KIM-1, R&D Systems MAB1817, 1:250) in block solution ...
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bioRxiv - Microbiology 2022Quote: Secretion of CXC motif chemokine ligand 10 (CXCL10/IP-10, DY266) and CC chemokine ligand 5 (CCL5/RANTES, DY278) were quantified by DuoSet® ELISA (R&D systems, Bio-Techne GmbH, Wiesbaden, Germany) under BSL-3 conditions according to the manufacturer’s instructions using the appropriate ancillary reagent kit (DY007).
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bioRxiv - Immunology 2022Quote: ... NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL rhIL-2 (R&D Systems, Cat. 202-IL-010). NK cells were then placed into a 37°C incubator ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... there were six mice per treatment and control group for a total of 78 mice in thirteen groups: (A) Seven vaccine groups included 1 μg of unformulated SARS-CoV-2 spike protein (R&D Systems, Cat# 10549-CV-100) delivered with adjuvanted NPs ...
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bioRxiv - Pathology 2020Quote: ... cells of 2 MOI were treated with serum-free media for 24 hours and then media containing TGF-β (20 ng/ml, R&D Systems, Minneapolis, MN, USA) for 72 hours ...
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bioRxiv - Physiology 2024Quote: Wild type C57BL/6 10-week-old female mice were treated daily with recombinant tumor necrosis factor-like weak inducer of apoptosis (TWEAK 1237-TW; R&D Systems; 2 ug per mouse) for 4 days followed by a single i.p ...
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bioRxiv - Immunology 2024Quote: ... 2fTGH and HEK293T cells and ELISA was performed according to the manufacturer’s protocols for human IFN-β (R&D systems DY814-05PBL Assay Science 41410-2), human IFN-γ (Biolegend 43104 ...
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bioRxiv - Molecular Biology 2023Quote: Polystyrene microtiter plates were coated with 1 µg/ml recombinant SARS-CoV-2 spike protein produced in HEK293T cells (10549-CV-100, R&D Systems and Biotech, NE, MN, USA) while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... The remaining NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL (250 IU/mL) rhIL-2 (R&D Systems, cat. no. 202-IL-010), then placed in a 37C CO2 incubator for 12-16 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were starved with 2% FBS media overnight and treated with Activin-A (R&D system, 338-AC-010, 25 ng/mL or 50 ng/mL) for indicated times.