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Citations for R&D Systems :
1051 - 1100 of 1389 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... chick GFP (1:500; 2010, Aves) and goat Contactin-2 (1:100; AF1714 R&D Systems, Minneapolis, MN, USA), Donkey anti-goat-488 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Autophagy was induced by treating the cells for 2 hours in 250nM Torin-1 (R&D Systems, 4247/10) in 1X Hanks’ Balanced salt solution ...
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bioRxiv - Immunology 2020Quote: ... 2-incubation with 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) in microscopy buffer for 1 h at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An ELISA for mouse lipocalin-2 was performed on feces (protocol according to the manufacturer; R&D Systems kit) to determine the inflammatory state of the gut.
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bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...
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bioRxiv - Microbiology 2024Quote: ... was measured from the resulting supernatant using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN). Measuring lipocalin-2 (Lcn-2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with compounds for 2 hours prior to TGF-β1 stimulation (1ng/ml, R&D systems. After 48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and the medium was supplemented with 10 ng/ml rhFGF-2 and 20 ng/ml VEGF (R&D Systems) for EC expansion ...
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL of IL-2 and 10 ng/mL of IL-12 (R&D systems, 419-ML-010)) ...
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bioRxiv - Immunology 2023Quote: ... MRT organoids were cultured in droplets of growth factor-reduced basement membrane extract (BME) Type 2 (R&D Systems) in kidney organoid medium (KOM ...
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bioRxiv - Cell Biology 2023Quote: ... starved for 2 hours and treated with 10 ng/ml rat recombinant EGF (R&D Systems, Minneapolis, MN, USA) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Cell Biology 2023Quote: ... to small clumps and plated on a layer of BME type 2 (R&D Systems, cat. no. 3533-001), incubated overnight in culture medium supplemented with 10 μg/mL polybrene (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... and the growth factors FGF-2 (20 ng/ml, Alomone Labs) and EGF (20 ng/mL, R&D systems). NSC differentiation was induced withdrawal of these growth factors.
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bioRxiv - Immunology 2023Quote: ... mouse IFNβ (catalog # 42400-2) cytokine levels were measured using ELISA kits from R&D Systems (Minneapolis, MN, USA). Human IFNβ levels were measured using an ELISA kit (PBL Assay Science ...
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bioRxiv - Cancer Biology 2024Quote: ... Each microwell was then overlaid with 2 μL of organoid digestion mix including Organoid Harvesting Solution (R&D Systems) with 10 mg/mL B ...
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bioRxiv - Immunology 2024Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems, 10549-cv) was determined by injecting two to four concentrations of SARS-CoV-2 RBD (50 nM highest concentration) ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured using recombinant Human IL-2 (R&D Systems, catalog no. 202-GMP or BT-002-GMP) supplemented at 300 IU/mL and stimulated with 12.5 uL/mL Immunocult Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
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bioRxiv - Immunology 2021Quote: ... 115-006-068), 250ng/ml mouse recombinant MEGACD40L (Enzo, ALX-522-120) and 20ng/ml Recombinant mouse IL-4 (R&D system, 404-ML). Cells were collected at specific time points for analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µm sections were deparaffinized and stained with either hematoxylin and eosin or with specific antibodies for PROX1 (R&D Systems, AF2727) and GFP (Abcam ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...