Labshake search
Citations for R&D Systems :
1301 - 1350 of 2638 citations for IL 4 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma IL-6 was assessed in duplicate using high-sensitivity enzyme-linked immunosorbent assay (ELISA; Quantikine HS Human IL-6 ELISA, R&D Systems, UK) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: The secreted TNF-α and IL-6 cytokines in the culture supernatants were quantified by ELISA using Duo Set ELISA (R&D systems) as per the manufactures instructions ...
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bioRxiv - Cell Biology 2022Quote: The level of IL-22 in the patients’ serum were measured by ELISA kits (Quantikine ELISA kits, R&D Systems; Minneapolis, MN, USA). The absorbance of each well was measured with a microplate reader (Multiskan FC ...
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bioRxiv - Cancer Biology 2020Quote: ... condition media was collected and estimated quantity of the secretary IL-6 using commercially available ELISA kit (R&D Systems, DY 206-05) with human IL6 antibody (R&D Systems ...
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bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
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bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
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bioRxiv - Immunology 2022Quote: Detection of secreted IL11 into the supernatant of HPAEpiC cultures was performed using the human IL-11 ELISA kit (R&D systems, D1100) according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were then blocked with 10% normal goat serum and incubated with primary antibodies against Iba1 (1:500, Wako Chemicals, USA) and IL-1β (1:1000, R&D Systems, USA) overnight at 4°C ...
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bioRxiv - Pathology 2022Quote: ... for 1 hour at room temperature followed by overnight incubation with primary antibodies for IL-31RA (15 µg/ml, R&D Systems AF2769) and CHRM3(1:500 ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... the conditioned media was stored at -80° C and the interleukin-6 (IL-6) content was quantified via enzyme-linked immunosorbent assay (R&D Systems, DY406) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems).
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bioRxiv - Immunology 2023Quote: ... and hESCs were seeded at 3×105 cells/well onto 6-well plates and rested for 24hrs prior to stimulation with PBS or rhIL-23 (1290-IL-010, R&D Systems, USA) at concentrations of 1 ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-ATG12 (Cat# MAB6807) and recombinant mouse IFN-α (Cat #:12100-1) from R&D Systems (Minneapolis, MN 55413, USA). The mouse anti-CHIKV (Clone A54Q ...
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bioRxiv - Cancer Biology 2020Quote: ... neutralizing Ab anti-human/mouse SDF-1 25μg/ml (MAB310) and mouse VEGF 150ng/ml (VEGF164) (all from R&D System).
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bioRxiv - Immunology 2020Quote: ... Neutrophil migration in response to UV light was inhibited by treating mice intraperitoneally (i.p.) with 50 μg anti-mouse G-CSF monoclonal antibody or mouse IgG isotype control (R&D Systems) 24h and 3h prior to skin exposure to UVB light (1 x 500 mJ/cm2 ...
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bioRxiv - Immunology 2021Quote: ... recombinant proteins representing either full-length mouse ANGPTL3 or the N-terminal domain of mouse ANGPTL3 (R&D Systems, Minneapolis, MN), or the N-terminal domain of human ANGPTL4 (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... anti-FcγRII/III (rat anti-mouse, clone 190909, cat. MAB1460), and anti-FcγRI (rat anti-mouse, clone 29035, cat. MAB2074) were purchased from R&D systems. 40μg of anti-FcγRII/III and 20μg of anti-FcγRI was used in FcγR-blocking conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... CTSD for mouse cell lysates (#MAB1123-SP) and CTSA for mouse cell lysates (AF1029-SP) were bought from R&D Systems.
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bioRxiv - Microbiology 2021Quote: ... 5 ng/mL BMP-4 (314-BP-050, R&D Systems), 50 ng/mL VEGF (293-VE-500 ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant 52-kDa pro-cath-D (4 µM; R&D Systems) was auto-activated to 51-kDa pseudo-cath-D in 0.1 M Na-acetate buffer (pH 3.5) ...
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bioRxiv - Biochemistry 2022Quote: ... 20 ng/mL bone morphogenetic protein 4 (BMP4, R&D Systems), and 10 ng/mL fibroblast growth factor-basic (bFGF ...
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bioRxiv - Immunology 2020Quote: ... 3 and 4 were measured using Luminex technology (R&D Systems), according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 ng human recombinant IGF2 (292-G2-050, R&D Systems) was used as positive control ...
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bioRxiv - Cell Biology 2021Quote: ... 30 ng/ml Bone morphogenic protein-4 (BMP4) (R&D systems), 1.5 μM CHIR99021 (Stemgent) ...
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bioRxiv - Developmental Biology 2022Quote: ... BMP-4 (40 ng/ml, R&D Systems #5020-BP-010) or CHIR99021 (3 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... The caspase 4 inhibitor (Z-YVAD-FMK, FMK005, R&D systems) was used in further experiments at the dose of 50 µM ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µl of anti-V5-AF647 antibody (R&D Systems, FAB8926R) was used for staining cells for 20 minutes on a shaker at 500 rpm (Thermo Microplate Shaker) ...
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bioRxiv - Molecular Biology 2023Quote: ... and growth factors: 5 ng/ml BMP-4 (R&D Systems), 9 ng/ml Activin A (R&D Systems) ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 ng/mL human recombinant FGF-4 (R&D Systems). After 9 to 13 days ...
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bioRxiv - Microbiology 2021Quote: ... After centrifugation, supernatants were used for ELISA analysis (Mouse C3a (TECOmedical, TE1038) or Mouse IFN-γ DuoSet ELISA (R&D Systems, DY485)) ...
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bioRxiv - Immunology 2020Quote: ... We used commercially available ELISA kits to quantify plasma IFNγ (mouse IFNγ platinum ELISA extra sensitive, ebioscience), CXCL10 (mouse IP-10 platinum ELISA, ebioscience) and Angiopoietin-2 (mouse/rat Angiopoietin-2 quantine ELISA kit, R&D Systems). Absorbance was measured using a Multiskan Ascent (MTX Lab systems ...
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bioRxiv - Neuroscience 2021Quote: The cytoplasmic fraction of human postmortem brain homogenates and astrocyte culture media were used to determine cytokine content using Mouse or Human Proteome Profiler arrays (Mouse Cytokine Array Panel A and Human Cytokine Array, R&D Systems), according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-mouse Lyve1 (1:500, #DP35135P, Acris Antibody GmbH, Herford, Germany) and sheep anti-mouse Trem2 (1:50, #AF1729, R&D Systems), and rabbit anti-mouse Ki67 (1:200 ...
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bioRxiv - Bioengineering 2020Quote: The relative cytokine content of the decellularized mouse heart tissues (n=3) were obtained using the Mouse XL Cytokine Array Kit (ARY028, R&D Systems). Briefly ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of TGFβ secreted by mouse pancreatic tumor cells was quantified by using the Mouse TGFβ 1 DuoSet ELISA (#DY1679-05, R&D systems), complemented with Sample Activation Kit 1 (#DY010 ...
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bioRxiv - Cancer Biology 2021Quote: CCL2 and CXCL1 were also quantified with Mouse CCL2/JE/MCP-1 and Mouse CXCL1/KC DuoSet ELISA kits (R&D Systems), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... Cxcl10 and Ccl22 in mouse peritoneal cells were analyzed by ELISA according to the manufacturers instructions (Human and Mouse DuoSet ELISA respectively, R&D Systems). For relative chemokine concentration in comparison to parasite load ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescently-labeled antibodies directed against cell-specific surface marker for 30 min one ice in the dark (anti-mouse CD45-APC/Cy7, #103116, BioLegend; anti-mouse CD45-FITC, #FAB114F, R&D Systems, Wiesbaden-Nordenstadt ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then washed with phosphate-buffered saline (PBS)/0.05% Tween 20 and incubated with mouse IgG2a-anti-IAV NP (Clone Hb65, EVL laboratories) 5 μg/ml or mouse IgG2a isotype control (MAB003, R&D systems) 2.5 μg/ml in PBS/0.1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... CXCL9 (5 μg, MAB392), mouse IgG1 isotype control (5 μg, MAB002) and mouse IgG2B isotype control (MAB004) all from R&D Systems, at RT for 2 h ...
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bioRxiv - Bioengineering 2023Quote: Cytokine profiling of the MBVs isolated from the aged and young mouse breast ECMs was performed using the dot blot-based Proteome Profiler Mouse XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions ...