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Citations for R&D Systems :
1151 - 1200 of 2638 citations for IL 4 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Concentrations of murine IL-11 in the culture supernatants were determined by ELISA according to the manufacturers’ instruction (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Human IL-8/CXCL8 Quantikine ELISA kit and non-immune human IgG control were purchased from R&D Systems (Minneapolis, MN). Phorbol myristate acetate (PMA) ...
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bioRxiv - Systems Biology 2022Quote: ... Levels of lymphocyte-derived cytokines (IFNγ, IL-17) were determined after 7 days (PeliKine Compact or Duoset ELISA, R&D Systems). Baseline inflammatory plasma markers from both cohorts ...
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bioRxiv - Microbiology 2019Quote: The human IFN-β ELISA Kit (cat. #41410-1) and IL-6 ELISA kit (cat. #D6050) purchased from R&D systems were used to measure the Chlamydia-induced IFN-β and IL-6 respectively ...
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bioRxiv - Immunology 2019Quote: ... the cells were transferred to an IL-17A ELISPOT plate for an additional 24hours and then developed according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Microbiology 2020Quote: ... Released IL-8 was determined in supernatants collected at 24 hrs using an ELISA kit (Biosource Europe and R&D Systems), according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml soluble anti-CD28 and cultured in the absence (Th0) or presence of 2.5 ng/ml IL-12 (R&D Systems) (Th1 ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: Supernatants harvested from infected cells were assayed using enzyme-linked immunosorbent assay (ELISA) kits for human IL-18 (R&D Systems) and IL-1β (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: Harvested supernatants from infected human macrophages were assayed for cytokine levels using ELISA kits for human IL-1α (R&D Systems), IL-18 (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... A microtiter plates with 96 well were incubated with anti-TNF-α or anti-IL-1β primary antibodies (R&D Systems) diluted in PBS (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were cultured for 8 days and stimulated with 2.5 ng/mL crude peanut extract (Stallergenes Greer) with or without 50 ug/mL anti-human IL-4Ra (R&D Systems), as previously described (17).
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bioRxiv - Molecular Biology 2024Quote: ... IL-10 and CCL7 were measured using a multiplex Luminex bead assay by R&D Systems (R&D Systems, Minneapolis, MN). CCL2 and TL1A were measured using human CCL2 (MCP-1 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at 1.5×105/ml in primary differentiation medium supplemented with 1ng/ml interleukin-3 (IL-3, R&D Systems), 10ng/ml SCF and 1μg/ml doxycycline ...
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bioRxiv - Immunology 2024Quote: ... Mice were injected with 100 ng/mL IL-1β (R&D Systems, #401-ML-005, 100 µg/mL stock in PBS) and 50 ng/mL TNF (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: AREG-neutralising experiments were performed by pre-incubating freshly prepared EVs (at 4 x 103 EVs per recipient cell) with either 4 µg/ml of goat anti-AREG (R&D Systems #AF262) or goat anti-IgG (R&D Systems #AB-1080-C ...
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bioRxiv - Immunology 2021Quote: ... Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453, R&D Systems, Minneapolis, MN).
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bioRxiv - Microbiology 2024Quote: ... KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453, R&D Systems, Minneapolis, MN). Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Immunology 2022Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Biochemistry 2019Quote: ... Cytokines (IL-6 and KC) were quantified by an enzyme-linked immunosorbent assay (Quantikine; R&D Systems Europe, Abingdon, Oxon, United Kingdom).
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Immunology 2022Quote: ... Human CSF-1 and IL-34 human serum levels were quantified using ELISA kits (both R&D systems, Mountain View, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Neuroscience 2020Quote: ... Serum levels of IL-17B and CXCL5 were measured in duplicate using a custom assay on the Luminex platform (R&D Systems). The manufacturer protocol was followed and antigen binding within the assay was measured on a Luminex 200 System and analyzed using Milliplex Analyst 5.1 ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma interleukin (IL)-6 and lipopolysaccharide-binding protein (LBP) levels were measured by commercially available enzyme-linked immunosorbent assay (R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2021Quote: ... The supernatants were collected 72 hours post-stimulation and the levels of IFNγ and IL-10 determined by and ELISA (R&D Systems). IFNγ production was also assessed by ELISPOT ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... were measured by flow cytometry 2 days after activation with or without an extra stimulation with 25 ng/mL IL-2 (R&D Systems) during 15 min.
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).