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Citations for R&D Systems :
1251 - 1300 of 3554 citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Adherent cell were then cultured in RPMI supplemented with 10% heat-inactivated human serum and L-Glutamine and gentamycin as above (RPMI10%HuS) and 30 ng/ml GM-CSF (Immunex) and 10 ng/ml IL-4 (R&D Systems) for 5 days50 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 106 BM cells were incubated at 37°C or 4°C for 45 min with or without Cxcl12 (10 nM, R&D Systems). The reaction was stopped by adding ice-cold RPMI and brief centrifugation at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Cancer Biology 2024Quote: A pool of plasma from 2 to 4 mice was loaded on the membrane of the Proteome Profiler Mouse XL cytokine Array (ARY028, R&D systems) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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bioRxiv - Physiology 2024Quote: ... the platelets were incubated at 4°C over night with antibodies against CXCR4 (MAB172, Clone # 44716, R&D Systems, Minneapolis, Minnesota, USA), ACKR3 (ab72100 ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (Cell Signaling; #5679S, western blot dilution 1:1000, IHC dilution 1:100; R&D Systems; AF2517, IF dilution 1:100), NR5A2 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... prior to 1 hour incubation of CD206 (1:200, R&D systems, AF2535), F480 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 ng/mL neuregulin-1 (recombinant human NRG-1; R&D Systems)) at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-βIII-Tubulin mAB (clone TuJ-1, 1:1000, R&D Systems MAB1195). The membranes were incubated with the primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD31/PECAM-1 (dilution 1:300, goat polyclonal, AF3628 R&D Systems) + anti-goat AF546 (dilution 1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal anti-goat TIMP-1 (R&D Systems; Minneapolis, MN; 1:300 dilution), monoclonal anti-mouse microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2019Quote: ... and Mfge8 (Thermo MA5-23913, 1:50; R&D Systems MAB2805, 1:50). All Ltf and Mfge8 antibodies have been previously validated by staining lactating mammary glands.
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bioRxiv - Cell Biology 2021Quote: ... IGF-1 (mouse/rat IGF-1 ELISA kit, R&D system, Minneapolis, MN) and total thyroxine (total thyroxine ELISA ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies Nkx6.1 (1:100, DSHB) and Pdx1 (1:100, R&D Systems) were added and incubated for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... CINC-1 and TIMP-1 were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Neuroscience 2023Quote: ... recombinant murine TIMP-1 (rmTIMP-1, R&D Systems, Minneapolis, MN; 10ng/mL) was added 24 hours prior to harvesting of protein/RNA.
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bioRxiv - Neuroscience 2023Quote: ... TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit, R&D Systems, catalog # MTM100) and MCP-1 (Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit ...
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bioRxiv - Neuroscience 2023Quote: Exogenous IGF-1 Administration: Human recombinant IGF-1 (R&D Systems 291-G1) was reconstituted in sterile saline and 5µls (30µgSE ...
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bioRxiv - Bioengineering 2023Quote: ... and goat anti-jagged 1 (1:150, AF599, R&D Systems, RRID: AB_2128257). Secondary antibodies (all raised in donkey ...
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bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated in primary antibodies (Anti-LAMP1 1:100 Invitrogen CAT#14-1079-80; anti-Iba-1 1:250 Abcam CAT#ab5076; anti-dsDNA 1:250 Millipore CAT#MAB1293; anti-PTDGS/PGD2 R&D Systems CAT#MAB10099 1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked in PBS and Tween-20 with 1 % (w/v) nonfat dry milk for 1 h at RT followed by incubation with 1:50 PAX6 (AF8150; R&D Systems) or 1:500 FOXC1/2 (CPA3482 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Physiology 2020Quote: ... non-esterified fatty acid (NEFA) serum levels were measured by colorimetric assay (Randox, UK) and dipeptidyl peptidase 4 (DPP4; R&D Systems, UK) levels were measured by ELISA.
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific, 114.13D; R&D Systems, MAGM204) were intravenously injected to an actin-DsRed mouse ...
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bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...