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Citations for R&D Systems :
1251 - 1300 of 3996 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the buffer was switched to imaging media with either 1mM or 11mM glucose with treatment (CB-100: 5μg/mL, nidogen-2 (40ng/mL, Biotechne R&D Systems)) and incubated for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatants were used with the Human Phospho-EphB4 DuoSet IC ELISA kit (#DYC4057-2, R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... 2 µM parkin (either full-length or truncated parkin) were incubated in the presence of 10 µM UbVS (R&D Systems) in 30 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor cells were resuspended in 1x PBS and mixed with methylcellulose (1:1 ratio) (R&D Systems). 8- to 10-week old naïve C57BL/6 mice were anesthetized with isoflurane and placed in a stereotactic frame ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human sFRP-1 and antihuman Dkk-1 antibodies (cat# CCM017; R&D Systems; Minneapolis, MN, USA) for 15 days ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor® 647-conjugated anti-human lymphatic vessel endothelial receptor-1 (LYVE-1 ,R&D Systems) or Alexa Fluor™ 488-conjugated anti-human laminin (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used: purified goat anti mouse IGFBPL-1 (R&D Systems, product no: AF4130, (1:50)) ...
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bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated with 50 µL of 1:1 Poly- L-Lysine (R&D Systems, #3438-100-01) and Poly-D-Lysine (R&D Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-mouse CD142 (Sino Biological, 50413-R001, 1:100; or R&D Systems, AF3178, 1:50). Anti-mouse CD142 antibodies were conjugated with Biotium Mix-n-Stain CF647 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-CD31/platelet-endothelial cell adhesion molecule-1 (PECAM-1) antibody (R&D Systems, AF3628,1:500), and rabbit anti-glial-fibrillary acidic protein (GFAP ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg/mL Noggin and 1 μg/mL Chordin as double treatment (758-CN; R&D Systems), 2% v/v of PBS containing 0.1% BSA as the control Noggin and Chordin double treatment ...
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bioRxiv - Immunology 2024Quote: ... human recombinant IFN-α2a (1 µg/ml) and IFN-β (1 µg/ml) proteins (R&D Systems) were reduced using NuPAGE Sample Reducing Agent (10x ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were administered either a 5 μL volume of phosphate-buffered saline (PBS) containing 10 μg/kg of carrier-free rmIL-1β (R&D Systems, Minneapolis, MN) or PBS alone (control ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Cell Biology 2020Quote: ... were covered with HUVECs and embedded 24 hours later in a 2,5 mg/mL fibrin gel in the presence or absence of VEGFA-165 at 5 ng/mL (R&D systems, Minneapolis MN, USA). Conditioned media from papillary or reticular fibroblasts were added on top of the gel and renewed every day for four days ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and HEK293T cells (ATCC) were cultured in DMEM (4.5 g/liter glucose, pH 7.2) with 5% FBS (Atlanta Biologicals/R&D Systems #S11150, Lot #F18071) 20 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Genetics 2023Quote: ... with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM, 10 µM or 30 µM, R&D Systems, 6977/10) or in the absence of ascorbic acid (normally added to the reprogramming medium at 25 mg/ml ...
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bioRxiv - Physiology 2022Quote: ... and were further stratified to either treatment of 5 d/wk injections of recombinant sclerostin (0.1 mg/kg body mass) (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline control ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C in 5% CO2 in a humidified incubator in IMDM with phenol red supplemented with 10% fetal bovine serum (FBS) (R&D systems, Minneapolis, MN), 1mM Sodium Pyruvate ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... This medium is based on DMEM/F12 supplemented with 5% R-Spondin1-conditioned medium (made from Cultrex 293T R-spondin1-expressing cells; R&D Systems, MN, USA), 10% Noggin-conditioned medium (made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Immunology 2021Quote: ... Interleukin 4 (IL-4) and Interferon Gamma (IFNy) from R&D Systems/Bio-Techne (R&D Systems/Bio-Techne ...
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bioRxiv - Cancer Biology 2021Quote: ... culture media were changed to serum free media or serum free media with 2 ug/ml IGF2 antibody (AF-292, R&D Systems) for another 72 hours ...
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bioRxiv - Immunology 2021Quote: Five million of splenocytes per well in 12-well plates were cultured in 2.5 ml of complete DMEM medium (for details refer below) with 1000 U/ml of recombinant human (rh) IL-2 (202-IL-010, R&D Systems) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Bioengineering 2020Quote: Injured animals were randomly selected to receive intralesional injections of eCS matrix functionalized with the neurotrophic factors fibroblast growth factor (FGF-2; 50μg/mL; R&D Systems; 233FB025CF) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Cell Biology 2020Quote: Relative phosphorylation levels of 43 kinases and 2 related proteins were assessed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Slit2 concentrations in mice serum were determined by the sandwich ELISA method with the DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the coverslips were transferred to a 48-well plate and cultured in Neurobasal-A medium + 2% B27 + 50 ng/ml of neurturin (R&D Systems) + 50 ng/ml of neuronal growth factor (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... six- to eight-week old NSG mice were subcutaneously injected into the flank with 2×106 wildtype or pre-transduced A-673 or RD-ES EwS cells in Cultrex Basement Membrane Extract (R&D Systems) to enhance tumor formation ...
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bioRxiv - Immunology 2022Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of human rIL-2 (10U/ml, R&D Systems) for 4 additional days ...
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bioRxiv - Microbiology 2022Quote: ... then stained with 0.1 µg/sample Alexa Fluor 647-conjugated mouse-anti SARS-CoV-2 Spike S1 (R&D Systems, FAB105403R-100UG) at 4°C for 30 min in the dark ...
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary goat F(ab’)2 anti-mouse IgG-PE antibody used for flow cytometry was purchased from R&D Systems (F0102B) (Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... Quantitation of IL-2 muteins in mouse serum was performed using an electrochemiluminescent immunoassay with a biotinylated rat anti-mouse IL-2 (JES6-1A12, R&D Systems) as a capture reagent and a ruthenylated goat anti-mouse IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... and then differentiated into day 2 definitive endoderm through the addition of CDM2 basal medium supplemented with Activin A (100 ng/mL; R&D Systems), LDN-193189 (250 nM ...
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bioRxiv - Immunology 2021Quote: Plasma cytokine and chemokine profiles from Mif-2−/−Apoe−/− mice and Apoe−/− mice were mapped by capitalizing on a membrane-based mouse cytokine array panel A (R&D Systems) following the standard instructions ...
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bioRxiv - Pathology 2021Quote: Frozen maternal serum aliquots were quick thawed and immediately assayed using either a Human ACE-2 DuoSet ELISA assay (R&D systems) or a ParameterTM Estradiol assay per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IL-21 and IL-15 were measured in plasma diluted 1:2 from acute COVID-19 patients and acute healthy controls using a custom designed multiplex Magnetic Luminex Screening assay (R&D Systems), according to the manufacturer’s instructions.