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Citations for R&D Systems :
1101 - 1150 of 3996 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Biochemistry 2020Quote: ... a total of 1 μg native proMMP2 and 0.5 μg proMMP14 were separately incubated in 10 μl with a range of furin (catalog no. 1503-SE-010, R&D systems) concentrations (0 – 250 nM ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Genetics 2023Quote: Reprogramming was done as described previously, combining the IFNγ (10 ng/ml, day 0 to 5) (R&D Systems, 485-MI-100) with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM ...
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bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Biophysics 2023Quote: Collagen fibrils were formed by combining rat tail tendon-derived acid-soluble type I collagen (5 mg/mL in 20 mM acetic acid) (CultrexRat Collagen I, R&D Systems) with 400 mM HEPES + 0.02% sodium azide and 1 M NaCl at room temperature to reach final concentrations of 1 mg/mL collagen ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were incubated with anti-Siglec-9-APC (Clone 191240) and/or anti-Siglec-5/14-AF488 (Clone 194128) (R&D Systems) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Molecular Biology 2022Quote: Human dermal fibroblasts transfected with either 10 nM si-circGLIS3 or scrambled siRNA (Eurofins Genomics, Ebersberg, Germany) for 48 hours and treated with 5 ng/ml TGF-β1 (R&D Systems) were rinsed and pelleted in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: The preosteoblast cells cultured in tissue culture plates were differentiated for 5 days and treated with Myostatin of 100 ng/mL (788-G8-010/CF R&D system), follistatin of 100 ng/mL (769-FS-025/CF ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then washed with phosphate-buffered saline (PBS)/0.05% Tween 20 and incubated with mouse IgG2a-anti-IAV NP (Clone Hb65, EVL laboratories) 5 μg/ml or mouse IgG2a isotype control (MAB003, R&D systems) 2.5 μg/ml in PBS/0.1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated in blocking solution (3% bovine serum albumin + 0.1% Triton X-100 + 5% Horse serum) at room temperature for 1hr and then with anti-OSMR beta (R&D Systems, AF662) antibody (10 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively, R&D Systems). Mouse IL-33 was quantified by ELISA Kit (Cat# 88-7333-22 ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice ...
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bioRxiv - Immunology 2024Quote: ... in either 10% chicken sera in 5% BSA/PBS (immunofluorescence) and probed with goat anti-human MPO (detects mouse MPO, AF3667 R&D Systems) rat anti-mouse CD45 (all leukocytes ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse interleukin-3 (IL-3) (R&D Systems, 403-ML) and 10 ng/mL mouse interleukin-6 (IL-6 ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant mouse ICAM-1-Fc and VCAM-1-Fc were purchased from R&D Systems. Antibodies for flow cytometery were from BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1) (1/100; R&D Systems; Cat. No. AF4534); and mouse anti-neurofilament protein (2H3 ...
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bioRxiv - Cell Biology 2021Quote: We quantified ET-1 levels using the Endothelin-1 Quantikine ELISA kit (R&D systems). First we diluted the media 1/125 in the appropriate buffer and used 75 μL of the dilution for the assay ...
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bioRxiv - Systems Biology 2020Quote: ... goat anti-PDGFRα (1:200, R&D Systems; mouse anti-APC (CC1; 1:300, Millipore); rabbit anti-GFAP (1:300 ...
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bioRxiv - Developmental Biology 2022Quote: ... and appropriate Alexa Fluor (Thermo 1:200) and Northern Lights (R&D Systems 1:200) conjugated secondary antibodies were incubated for 2 hours at 37ºC ...
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bioRxiv - Bioengineering 2020Quote: ... STRO-1 (1:100, animal poly- or mono-, R&D System, Inc., Minneapolis, MN, USA) and the extracellular markers anti-integrin β1 (1:50 ...
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bioRxiv - Cancer Biology 2021Quote: ... NKp46 (1:175, HIER 1, monoclonal mouse, clone 195314, R&D Systems, USA; MAB1850-500), FoxP3 (1:100 ...
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bioRxiv - Pathology 2021Quote: Endothelin-1 ELISA was performed on plasma using Endothelin-1 Immunoassay kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and SorCS2 (R&D systems, AF4237, 1:1000 in WB and 1:100 in ICC).
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bioRxiv - Molecular Biology 2021Quote: ... and MCP-1 (CCL2 /JE/MCP-1 ELISA kit, R&D Systems Inc., Minneapolis, MN) in mouse serum ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1) (1/100; R&D Systems; Cat. No. AF4534), and guinea pig anti-SCIP (1/16,000 ...
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bioRxiv - Bioengineering 2023Quote: ... human/mouse GLI-1 affinity purified polyclonal (1:100; AF3455, R&D systems, RRID: AB_2247710), RUNX2 (27-K ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... or CD31/PECAM-1 Alexa Fluor 488-conjugated Antibody (1:200; R&D system #FAB3628G). After primary antibody incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Glypican 1 (GPC-1) biotinylated antibody was purchased from R&D systems (MN, USA). Biotinylated anti-CD63 ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-FORKHEAD BOX PROTEIN 1 (FOXP1) (1/100; R&D Systems; Cat. No. AF4534); mouse anti-neurofilament protein (2H3 ...
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bioRxiv - Cell Biology 2024Quote: ... were coated with VCAM-1 (1 µg/mL) (cat no. 862-VC; R&D Systems) overnight at 4 °C.
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bioRxiv - Cell Biology 2022Quote: ... anti-m/rCD31/PECAM-1 Alexa Fluor 488 Conjugated (1:50, FAB3628G; R&D Systems); Claudin-5 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... 50 μL/well of a 1:1 solution of H2O2 (R&D Systems, part #895000) and tetramethylbenzidine (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Immunology 2022Quote: ... cells were washed at day 7 and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 250 U/ml IFN-α (R&D Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... organoids were grown embedded in a mix of 90% ice-cold RGF BME Type 2 PathClear (Cultrex, R&D Systems) and 10% Dulbecco’s modified Eagle’s Medium/Ham’s F12 (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Bioengineering 2021Quote: ... Virus infection was detected with mouse mAb to SARS-CoV- 2 nucleocapsid (N) (R&D Systems, Minneapolis, MN, Cat#MAB10474) and counterstained with rabbit anti-mouse IgG-HRP (Rockland Immunochemicals ...