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Citations for R&D Systems :
1201 - 1243 of 1243 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... +/-1 µg/mL anti-CD28 (CD28.2) antibody (eBioscience/Thermo 16-0289-81) or recombinant human CD58-Fc chimera protein (R&D Systems 10068-CD-050) at 37 ºC for 2 hours ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Systems Biology 2021Quote: ... we added one or combinations of the following recombinant proteins to the medium: 200 ng/mL of recombinant mouse FGF4 (R&D Systems, #7486-F4), 200 ng/mL of recombinant human FGF5 (R&D Systems ...
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bioRxiv - Immunology 2020Quote: Secreted IL-1b and MCP-1 proteins in supernatants of monocytic cells stimulated with TNF-α were quantified using sandwich ELISA following the manufacturer’s instructions (R&D systems, Minneapolis, USA).
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bioRxiv - Bioengineering 2020Quote: ... medium was removed and the scaffolds designated for study in normal growth media were placed in wells with fresh normal growth medium and the mineralized collagen-AM scaffolds designated for study in inflammatory conditions were placed in wells with inflammatory medium (1 ng/ml of IL-1β recombinant porcine protein (R&D Systems, Minneapolis, MN) added to normal growth media) ...
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bioRxiv - Cancer Biology 2020Quote: The relative content of 111 cytokine proteins (Table S1) was determined using the dot blot based mouse XL cytokine array kit following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2024Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1% v/v FBS with or without 100 ng/ml Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN). Medium change was performed at day 3 and images were taken at 3 and 5 days using a Keyence BZ-X800 Fluorescence Microscope (Keyence ...
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Neuroscience 2024Quote: ... BDNF standards and tissue protein levels were run in triplicate per manufacturer instructions using Total BDNF Quantikine ELISA Kit (Catalog #DBNT00, R&D Systems, Minneapolis, MN), a sandwich enzyme immunoassay technique ...
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bioRxiv - Cell Biology 2023Quote: ... Myeloperoxidase (MPO, indicative of tissue oxidation) levels in ALF (using 10 μg of protein) were determined by using LUMINEX (R&D Systems, Minneapolis, MN).
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bioRxiv - Neuroscience 2023Quote: ... Fractalkine protein levels were measured in hippocampal lysates via the R&D System’s Mouse Fractalkine (CX3CL1) Quantikine ELISA Kit (R&D Systems cat. Nr. MCX310) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
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bioRxiv - Bioengineering 2023Quote: ... proteins were extracted via lysis using a lysis buffer from the Proteome Profiler™ Human Apoptosis Array Kit (ARY009, R&D Systems, Minneapolis, MN) kit ...
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bioRxiv - Cancer Biology 2023Quote: ... chemokines and acute phase proteins were simultaneously assessed using the proteome profiler human XL cytokine array kit [ARY005B] from R&D Systems (Abingdon, UK). Cell lysates were obtained from A375 following treatment with 0.1 MOI of RT3D and 0.1 mM talazoparib at 48 hours post-treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... female BALB/c mice were anaesthetized and injected as described above with 1 μg CINC-1 protein (453-KC-050/CF, R&D systems, Abingdon, UK) in 1 μL saline in the left striatum ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-SARS-CoV-2 spike S2 subunit mAb (IgG2a, Cat#: MAB10557) and mouse anti-SARS-CoV-2 nucleocapsid protein mAb (IgG2b, Cat#: MAB10474) were from R&D Systems (Minneapolis, MN). Mouse anti-SARS-CoV-2 membrane protein mAb E5A8A (IgG1 ...
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bioRxiv - Genomics 2024Quote: FACS-sorted Vcam1-FAPs were grown to 60-80% confluence followed by adipogenic differentiation and were treated with 500 ng/mL recombinant mouse sFRP-1 protein (R&D systems, 9019-SF) with or without sFRP-1 inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... Live cells were used for staining the cell surface proteins KP by incubating with FACS buffer containing PE-conjugated KDR antibody (R&D Systems FAB357P-100) and APC-conjugated PDGFRa antibody (R&D Systems FAB1264A ...
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bioRxiv - Cell Biology 2024Quote: ... ranging from 0.1 μg/mL to 10 μg/mL, of purified human SNED1 proteins (full-length, fragments, or integrin-binding mutants) or murine Sned1 (R&D systems, 9335-SN) for 180 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 107 cells per sample were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and incubated with 50 µg/mL SARS-CoV-2 spike S1 subunit-Fc fusion protein (R&D systems, #10623-CV-100) or human IgG1 isotype control (BioXCell ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
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bioRxiv - Immunology 2020Quote: Soluble proteins were quantified in EDTA anticoagulated plasma using the Luminex® multiplex platform (Luminex, Austin TX) with custom-developed reagents (R&D Systems, Minneapolis, MN), as described in detail ((16 ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Gamma (10795-CV-100) and Delta (10878-CV-100) spike proteins were purchased from R&D Systems (R&D Systems, Bio-Techne, MN, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Biophysics 2023Quote: ... The reversible osmotic stress eliminates these wrinkles and allows the conversion of effective 3D protein concentrations into 2D receptor concentrations.(37) The swelling media was supplemented with 50 nM dimeric ephrinA1-Fc (R&D Systems, #602-A1-200). The cells were allowed to equilibrate for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots of individual OCY454 WT and Cx43 KO samples differentiated for ten days were assessed for key secretory proteins implicated in osteoblast and osteoclast differentiation – sclerostin (R&D Systems; Catalog #DY1589-05) and dickkopf-related protein 1 (R&D Systems ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Molecular Biology 2024Quote: ... Plasma concentrations of fatty acid binding protein 4 (FABP4) and leptin were determined using the xMAP technology Luminex assays (R&D Systems, Minneapolis, MN, USA) and the Luminex MAGPIX System (Luminex Corp. ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... there were six mice per treatment and control group for a total of 78 mice in thirteen groups: (A) Seven vaccine groups included 1 μg of unformulated SARS-CoV-2 spike protein (R&D Systems, Cat# 10549-CV-100) delivered with adjuvanted NPs ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Neuroscience 2024Quote: ... was assessed on 5μL murine plasma using the human SimplePlex Assay as the kit is cross reactive with murine NF-H (R&D Systems, Protein Simple SPCKB-PB-000519) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Recombinantly expressed TIM-4 in HEK293 cells (Q96H15) and podocalyxin-like protein 1 recombinantly expressed in NS0 cells (AAB61574.1) were purchased from R&D Systems (9407-TM, 1658-PD-050). Recombinantly expressed LAMP-4 in HEK293 cells (P34810 ...
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bioRxiv - Physiology 2024Quote: The expression patterns of proteins associated with angiogenesis were assessed utilizing the Proteome Profiler™ Human Angiogenesis Antibody Array Kit (R&D Systems, Minneapolis, MN, USA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: Polystyrene microtiter plates were coated with 1 µg/ml recombinant SARS-CoV-2 spike protein produced in HEK293T cells (10549-CV-100, R&D Systems and Biotech, NE, MN, USA) while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
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bioRxiv - Genetics 2024Quote: ... The inhibitors of Wnt-mediated osteoblastic bone formation Dickkopf related protein-1 (DKK-1) and Sclerostin (SOST) were determined by ELISA at P183 (R&D Systems Europe Ltd, Abingdon, Oxfordshire, UK).
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bioRxiv - Physiology 2024Quote: ... Protein expression was visualised using immunofluorescence staining of mesenteric artery rings and the following antibodies: anti-CD31 (PECAM, # AF3628, R&D Systems, 1:1000 dilution, raised in goat); anti-TRPV4R (# ACC-034 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...