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Citations for R&D Systems :
1051 - 1100 of 1243 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Tumor lysates were quantified and 40 μg protein loaded for analysis of GzB levels by ELISA (R&D Systems).
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells surface ACE2 protein was stained with Alex Flour® 647-conjugated human ACE2 antibody (R&D Systems FAB933R). Live/Dead (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Immunology 2020Quote: ... anti-CD28 (1 µg/ml, clone CD28.2, eBioscience) and immobilized recombinant ICAM1-Fc fusion protein (0.5µg/ml, R&D Systems) in complete RPMI/HS medium ...
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bioRxiv - Neuroscience 2022Quote: ... Primary microglia were stimulated with recombinant Mouse CXCL10 protein (R&D Systems 466-CR-608 050/CF, Minneapolis MN) and incubated with Mouse CXCL10 antibody (R&D Systems AF-466-609 NA ...
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bioRxiv - Cell Biology 2023Quote: ... Direct binding of decorin protein core to the soluble Fc tagged VEGFR3 (sVEGFR3-Fc, R&D systems, 349-F4), was performed using solid-phase binding assay ...
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bioRxiv - Immunology 2022Quote: ... in absence or presence of stimuli (PMA/ionomycin or human recombinant soluble BAFF/BLyS/TNFSF13B Protein (R&D System)) for 18 h at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μL of sample containing either LNCaP crude extract or recombinant HK2 protein (R&D Systems™, Cat# 8179HK020) was added to a well of 96-well plates ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Physiology 2024Quote: ... The levels of GDF15 protein in the serum were measured by an ELISA kit (Cat# MGD150, R&D Systems) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... NSCs were treated in the presence or absence of the recombinant proteins RSPO1 (7150-RS/CF, R&D Systems), RSPO2 (6946-RS/CF ...
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bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...
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bioRxiv - Molecular Biology 2022Quote: ... Day 0: 10 ng/ml recombinant human FGF basic/FGF2/bFGF (146 aa) protein (R&D Systems 233-FB), 10 µM RI (what is RI?) ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentration of human HGF was determined by ELISA using the Quantikine Human HGF Immunoassay (R&D Systems #DHG00B) according to manufacturer instructions and analyzed by a SpectraMax ID3 microplate reader ...
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bioRxiv - Cancer Biology 2023Quote: MSCs were stimulated with recombinant human IFNα (0.03 U/ml) or IFNβ (60 pg/ml) protein (R&D Systems), in combination with different concentrations of inhibitor of IFNα/β (i-IFNα/β ...
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bioRxiv - Immunology 2024Quote: ... were suspended in 200 μL of a 125 nM solution of recombinant human SIRPα-Fc protein (R&D Systems), the cognate isotype control recombinant human IgG1 protein (Sino Biological) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 50 ng/mL human bone morphogenetic protein 4 (hBMP4) (R&D Systems, Minneapolis, Minnesota, USA), 50 ng/mL human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Immunology 2024Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems, 10549-cv) was determined by injecting two to four concentrations of SARS-CoV-2 RBD (50 nM highest concentration) ...
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bioRxiv - Cell Biology 2024Quote: ... were coated overnight at 4°C with 5 ug/ml recombinant Invasin proteins or 2% BME (R&D systems) in cold PBS milliQ ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981; IFNλ2/3: R&D systems, MAB1587), or mock ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Neuroscience 2024Quote: ... HEK-293 cells 72h posttransfection or adult DRG neurons at DIV 3 were incubated with commercial anti-CASPR2 antibodies (AF5145, 1:250, R&D Systems, Minneapolis, MN, US) and/or human patient serum (1:50-1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations was determined by Thermo Fisher BCA assay kit and then 50μg of protein was loaded into the pre-coated VEGFA ELISA kit (R&D systems). Assay per manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
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bioRxiv - Zoology 2022Quote: ... The transfected cells were cultured in iPSC medium with 200 ng/mL of recombinant viral B18R protein (R&D Systems). B18R was removal once the transfected cells were ready to re-seed onto irradiated MEFs.
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of angiogenic proteins in CM was measured using a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D systems) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Cancer Biology 2023Quote: ... 200μg of total mouse protein samples were analyzed respectively with a Mouse XL Cytokine Array Kit (R&D Systems; ARY028) per manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: YM1 and CCL17 protein levels were measured in PCLS (4mm diameter) supernatant using commercially available ELISA kits (R&D Systems), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). The supernatant and organoid lysates were collected to measure GLP-1 concentrations in secretion and total protein concentrations ...
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bioRxiv - Microbiology 2024Quote: ... supernatants were collected for protein evaluation performed with enzyme-linked immunosorbent assay (ELISA) kit (R&D System, Inc. Bio-Techne) according to manufacturer’s instruction in order to assess human IL-6 (#DY206) ...
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bioRxiv - Immunology 2024Quote: INFα/β protein concertation in mice WT and KO-Hmox1 blood serum was measured using ELISA kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Nunc® Maxisorp™ 384-well plates were coated with 6 µg/ml of recombinant mouse pIgR protein (R&D Systems) diluted in coating solution concentrate (Seracare ...
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bioRxiv - Microbiology 2022Quote: ... KC protein from mouse brain homogenates was detected by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Cell Biology 2021Quote: We performed a Phage-Fab (antigen-binding fragment) selection using a naïve Fab library (libF46) on an immobilized recombinant rat Unc5B-ECD Fc fusion protein (R&D systems). Phage particles fused with Fabs were incubated with an unrelated protein (e.g ...