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Citations for R&D Systems :
1101 - 1150 of 1278 citations for IL 4Ra Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the cells were transferred to an IL-17A ELISPOT plate for an additional 24hours and then developed according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... The cytokine concentrations in the culture supernatant were determined using quantitative ELISA (Mouse TNF and IL-10 DuoSet; R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Released IL-8 was determined in supernatants collected at 24 hrs using an ELISA kit (Biosource Europe and R&D Systems), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml soluble anti-CD28 and cultured in the absence (Th0) or presence of 2.5 ng/ml IL-12 (R&D Systems) (Th1 ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: ... Sanofi-aventis Cat# NAC2004–5843-01) and interleukin-4 (IL-4; 1000 IU/mL; R&D Systems Cat# 204-1 L). Mature ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Mice were injected with 100 ng/mL IL-1β (R&D Systems, #401-ML-005, 100 µg/mL stock in PBS) and 50 ng/mL TNF (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-10 and CCL7 were measured using a multiplex Luminex bead assay by R&D Systems (R&D Systems, Minneapolis, MN). CCL2 and TL1A were measured using human CCL2 (MCP-1 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at 1.5×105/ml in primary differentiation medium supplemented with 1ng/ml interleukin-3 (IL-3, R&D Systems), 10ng/ml SCF and 1μg/ml doxycycline ...
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bioRxiv - Neuroscience 2022Quote: ... The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) ...
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bioRxiv - Microbiology 2023Quote: Supernatants harvested from infected cells were assayed using enzyme-linked immunosorbent assay (ELISA) kits for human IL-18 (R&D Systems) and IL-1β (BD Biosciences).
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bioRxiv - Microbiology 2023Quote: Harvested supernatants from infected human macrophages were assayed for cytokine levels using ELISA kits for human IL-1α (R&D Systems), IL-18 (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... A microtiter plates with 96 well were incubated with anti-TNF-α or anti-IL-1β primary antibodies (R&D Systems) diluted in PBS (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
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bioRxiv - Cell Biology 2024Quote: ... IL-1β and TNFα in plasma were measured using ELISA kits (DY410-05, DY406-05, DY401-05, R&D Systems, MN) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Immunology 2022Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Biochemistry 2019Quote: ... Cytokines (IL-6 and KC) were quantified by an enzyme-linked immunosorbent assay (Quantikine; R&D Systems Europe, Abingdon, Oxon, United Kingdom).
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of secreted IL-33 in the conditioned media was visualized on a Proteome Profiler Mouse XL Cytokine Array (R&D Systems). The concentration of IL-33 secreted in the conditioned medium was measured on a V-PLEX Mouse Cytokine 19-Plex Kit (Meso Scale Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Immunology 2022Quote: ... Human CSF-1 and IL-34 human serum levels were quantified using ELISA kits (both R&D systems, Mountain View, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 1.25% of supernatant from Con-A-stimulated rat splenocytes (assay positive control), c) recombinant canine IL-4 (rcaIL-4, 360 ng/mL) (R&D Systems), d ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit, R&D Systems). All media was collected two days following media change ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2019Quote: ... Immature dendritic cells were prepared by treatment of monocytes with 500 U/ml human IL-4 and 800 U/ml human GM-CSF (R&D Systems) in CellGenix media for 6 days ...
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bioRxiv - Neuroscience 2020Quote: ... Serum levels of IL-17B and CXCL5 were measured in duplicate using a custom assay on the Luminex platform (R&D Systems). The manufacturer protocol was followed and antigen binding within the assay was measured on a Luminex 200 System and analyzed using Milliplex Analyst 5.1 ...
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bioRxiv - Immunology 2020Quote: ... Quantitation of IL-2 muteins in mouse serum was performed using an electrochemiluminescent immunoassay with a biotinylated rat anti-mouse IL-2 (JES6-1A12, R&D Systems) as a capture reagent and a ruthenylated goat anti-mouse IgG ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma interleukin (IL)-6 and lipopolysaccharide-binding protein (LBP) levels were measured by commercially available enzyme-linked immunosorbent assay (R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2021Quote: ... The supernatants were collected 72 hours post-stimulation and the levels of IFNγ and IL-10 determined by and ELISA (R&D Systems). IFNγ production was also assessed by ELISPOT ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IL-10 clone 948505 (MAB9184) and the isotype controls mouse IgG2a (MAB003) and mouse IgG1 (MAB002) (all from R&D systems).