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Citations for R&D Systems :
951 - 1000 of 1278 citations for IL 4Ra Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... IL-12p40 cytokine release was quantified using the Duo Set ELISA kit from R&D Systems according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... BMDMs were stimulated on day 7 with the appropriate doses of IL-6 (R&D Systems) and IL-10 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with biotin-linked anti-IL-1β detection antibody (R&D Systems, 1:1000), followed by horseradish peroxidase-conjugated streptavidin ...
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bioRxiv - Immunology 2023Quote: ... and IL-17 in the culture supernatant was determined using ELISA kits from R&D systems DY485 ...
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bioRxiv - Immunology 2024Quote: ... The recombinant human IL-1RA protein was purchased from R&D Systems (#280-RA-050/CF) and reconstituted in phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... FL5.12 cells were cultured in the presence of 10 ng/ml IL-3 (R&D Systems). Cell cycle exit in FL5.12 was achieved by washing the cells three times with PBS to remove IL-3 from the cells and by placing the cells in IL-3 free culture media for 48 h ...
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bioRxiv - Immunology 2024Quote: ... in conjunction with neutralizing antibodies against mouse IFNγ and IL-4 (also from R&D Systems). Culturing was performed in IMDM medium (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... mouse IL-1b/1F2 (DY401-05) and mouse TNF-α (DY410-05) from R&D Systems according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... IL-1β and TNF-α in culture supernatants were measured using kits from R&D Systems or Proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Biochemistry 2021Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
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bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody used was goat IgG anti-IL-1RA (1: 500, AF-280-NA, R&D Systems) and samples were incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CFSE-labelled cells were then resuspended in R10 media supplemented with recombinant IL-2 (R&D system) and 2-mercaptoethanol (Themo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies used were: goat anti-mouse IL-33 (AF3626, R&D Systems, Minneapolis, MN; 1:1000), rabbit anti-Muc5ac (gift from Dr ...
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bioRxiv - Cancer Biology 2021Quote: ... interleukin- 6 (IL-6) were determined using enzyme immunoassay (EIA) kits (R&D Systems, Minneapolis, MN, USA). The concentration of the above cytokines in culture medium was determined by comparing their optical density to the standard curve.
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bioRxiv - Microbiology 2021Quote: Harvested supernatants from infected cells were assayed using ELISA kits for human IL-1α (R&D Systems), IL-18 (R&D Systems) ...
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bioRxiv - Microbiology 2021Quote: Supernatants harvest from infected cells were assayed using ELISA kits for human IL-18 (R&D Systems) and IL-8 (R&D Systems).
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bioRxiv - Microbiology 2021Quote: IL-1β in BMDM supernatants was quantified using a murine ELISA kit (R&D Systems®, MLB00C) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... and IL-1β levels were evaluated in macrophage media using a standard sandwich ELISA (R&D Systems DuoSet ELISA Development System ...
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bioRxiv - Biochemistry 2022Quote: ... I used 10 ng/ m L recombinant human interleukin 1 (IL-1α; R&D Systems, Minneapolis, MN). All incubations were done at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... IL-10 concentration in human intestinal biopsy supernatants was measured by ELISA (#DY217B-05, R&D systems). TNFα was measured in human monocyte-derived macrophage culture supernatants by ELISA (#DY210-05 ...
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bioRxiv - Neuroscience 2020Quote: ... and IL-10 were detected using the method provided by the kit manufacturer (R&D Systems, USA). Optical densities were measured using a Model 680 microplate reader (Bio-RAD ...
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bioRxiv - Microbiology 2022Quote: Basal media was supplemented with 50 ng/μL IL-13 (R&D Systems, cat # 213-ILB-010) and replaced every 48 hours for the final two weeks of differentiation before infection for IL-13-treated cultures ...
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bioRxiv - Synthetic Biology 2020Quote: Levels of IL-1Ra in serum and paws were assessed by ELISA (Quantikine-IL1Ra, R&D Systems). Levels of paw and serum inflammatory mediators were evaluated using Luminex® (Mouse High Sensitivity T-cell discovery array 18-Plex ...
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bioRxiv - Immunology 2019Quote: ... Supernatant was collected and IL-1β levels were measured by ELISA (R&D systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2020Quote: ... and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... sCD25 were measured by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00) and IP-10 by Human IP-10 ELISA Kit (CXCL10 ...
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bioRxiv - Molecular Biology 2021Quote: ... was obtained from Cell Signaling (Frankfurt am Main, Germany) and goat anti-mouse Il-36α (AF2297, RRID:AB_355216) was from R&D Systems/Bio-Techne (Wiesbaden-Nordenstadt ...
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bioRxiv - Immunology 2020Quote: ... and mouse IL-10 cytokine concentrations were determined using ELISA DuoSet kits (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... and 100 ng/ml recombinant mouse IL-28B/IFN-λ3 (R&D Systems #1789-ML-025/CF). For select experiments ...
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bioRxiv - Cell Biology 2022Quote: ... cell-free supernatants were collected and then assayed for murine IL-1 β (MLB00C, R&D Systems) by ELISA kit (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Recombinant human IL-1β was obtained from R&D Systems (Abingdon, UK; Cat. No. 201-LB/CF) and recombinant murine IFNγ from PeproTech (Rocky Hill ...
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bioRxiv - Neuroscience 2022Quote: ... and 25 ng/ml (422.6 nM) Hyper-IL-6 (R&D Systems, Cat. no. 8954-SR-025) at day 45 of differentiation ...
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bioRxiv - Molecular Biology 2022Quote: ... Human TGF-β1 (240-B) and IL-1β (201-LB-005) were purchased from R&D systems.
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bioRxiv - Immunology 2022Quote: ... Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein (2 μg/ml, R&D Systems), Ultrapure LPS from E ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human IL-4 500 IU/mL and GM- CSF 800 IU/mL (both R&D systems) at a cell density of 1-2 x106 cells/mL for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... 48 and 96 hours in the presence or absence of recombinant human IL-1β (R&D systems), recombinant human IFNγ (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... and IL-13 was assessed as previously described (DY 404, DY405, DY413, R&D systems, Minneapolis, MN)51 ...
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bioRxiv - Immunology 2023Quote: Collected supernatants were assayed for IL-6 and IFN-β using mouse DuoSet kits (R&D Systems). Samples were diluted in PBS and incubated in a 96-well plate pre-coated with either IL-6 or IFN-β capture antibodies for 2h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were subjected to enzyme-linked immunosorbent assay (ELISA) for IL-1β (SMLB00C, R&D systems). The assays were performed as recommended by the manufacturers.
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bioRxiv - Bioengineering 2023Quote: ... The human IL-1β antibody and goat IgG HRP-conjugated antibody were purchased from R&D Systems. Potassium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... cells were stimulated or not with 100 ng/ml mouse IL-17 recombinant protein (R&D Systems) for 10 or 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... goat anti-mouse IL-1β polyclonal antibody (250 ng ml−1; R&D Systems, AF-401-NA) rabbit anti-mouse caspase-1 + p10 + p12 monoclonal antibody (1.87 µg ml−1 ...
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bioRxiv - Immunology 2023Quote: ... The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... Abs against the following proteins were used: IL-1β (R&D systems, AF-401-NA, 1: 1,000), caspase 1 p20 (Millipore ...
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bioRxiv - Immunology 2024Quote: ... Release was determined by using the human IL-6 DuoSet ELISA kit (DY206-05; R&D systems) in combination with ELISA Ancillary Reagent Kit 2 (DY008 ...
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bioRxiv - Cell Biology 2024Quote: ... the proinflammatory cytokine cocktail contained 25 ng/mL mouse IL-1β (R&D Systems; 401-ML-010), 50 ng/mL mouse TNF-α (R&D Systems ...