Labshake search
Citations for R&D Systems :
1101 - 1150 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
-
bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
-
bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
-
bioRxiv - Immunology 2024Quote: ... cells were maintained in resting conditions by culturing in simple R10 or R10 supplemented with 10 ng/mL of recombinant human interleukin-7 (IL-7, R&D Systems, cat. BT-007-010) for one week.
-
bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary antibody incubation was done overnight at 4 °C with following dilutions: anti-PLOD2 mouse at 1:500-1:1,000 (MAB4445, R&D Systems), anti-FERMT3 mouse at 1:500-1:1,500 (ab173416 ...
-
bioRxiv - Microbiology 2021Quote: Sterile tissue culture plates were coated overnight at 4°C with 5 µg/mL recombinant CD62P protein (R&D Systems, #137-PS-050). The following day the wells were washed 3X with PBS before being blocked with 1% BSA in PBS at room temperature (RT ...
-
bioRxiv - Cell Biology 2024Quote: ... for 30 min on a rotator at 4 °C and then transferred to a new tube with 5 μl TSC1 antibody (R&D Systems, MAB4379) or 25 μl mouse IgG Control (SC-2025 ...
-
bioRxiv - Bioengineering 2024Quote: ... and 10ng/ml of Bone Morphogenic Protein-4 (BMP-4: R&D system, #314-BP-050) on differentiation days 1 ─ 2 to initiate differentiation ...
-
bioRxiv - Biochemistry 2024Quote: ... 3C-D (mouse, R&D systems cat# MAB10026; 1:250). The following secondary antibodies were used at a 1:10,000 dilution (species and vendor indicated in parenthesis) ...
-
bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
-
bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
-
bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
-
bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... Fibers were incubated in plating medium consisting of 15% Fetal Bovine Serum (Hyclone) and 2 ng/ml bFGF (R&D Systems) in DMEM.
-
bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
-
bioRxiv - Neuroscience 2022Quote: ... Group 2 ligands were derived from human transcriptomic data and included: TGFβ2 (R&D Systems, Cat. 302-B2-002, 5ng/mL), NLGN1 (Sino Biological ...
-
bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed three times with PBST and incubated with 15 μl of DAB (3,3’-Diaminobenzidine) chromogen (2 ml of DAB chromogen (860001, R&D Systems) diluted in DAB chromogen buffer (860005 ...
-
bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
-
bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were treated for 2 hours prior to infection with the IC50 concentration of anti-ACE2 antibody (R&D Systems: AF933) determined for the cell lines used ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... media was changed daily with hindgut medium (RPMI 1640, 1X GlutaMAX, 2% defined FBS, 2µM CHIR99021 and 500ng/ml FGF4 (R&D Systems) until day 8 ...
-
bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells were pre-treated for 16 h with Type I Interferon (1000 Units/mL; R&D Systems, 11200-2) and treated with HMW Poly(I:C ...
-
bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
-
bioRxiv - Immunology 2022Quote: ... were determined by the CXCL2/MIP-2 DuoSet enzyme linked immunosorbent assay (ELISA) kit to the manufacturer’s instructions (R&D Systems). Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend ...
-
bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...
-
bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
-
bioRxiv - Cell Biology 2024Quote: ... The organoids were encapsulated in 50% Cultrex basement membrane extract type 2 (BME-II) (R&D Systems; catalog #3533-010-02) and maintained in advanced DMEM/F12 (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
-
bioRxiv - Microbiology 2024Quote: ... Isolated naive CD4+ T cells were resuspended in isolation media supplemented with recombinant IL-2 (R&D Systems, #202-IL-050) at 10 ng/mL (growth media ...
-
bioRxiv - Immunology 2024Quote: ... Abundance of LCN-2 and IL-17A/F were measured by Quantikine ELISA assays from R&D Systems (Minneapolis, MN, USA). BALF (50 μL ...
-
bioRxiv - Immunology 2024Quote: ELISA kits were used to quantified serum ferritin (Christal Chem) and soluble mouse CD25/IL-2 alpha receptor (R&D Systems) following manufacturers instruction.
-
bioRxiv - Neuroscience 2024Quote: ... small molecules (CHIR99021[2 mM, Axon, Groningen, Netherlands]; SB-431542[10 mM, Axon, Groningen, Netherlands]; noggin[0.5 mg/ml, R&D Systems R&D Systems ...
-
bioRxiv - Cell Biology 2024Quote: ... the buffer was switched to imaging media with either 1mM or 11mM glucose with treatment (CB-100: 5μg/mL, nidogen-2 (Biotechne R&D Systems)) and incubated for 30 minutes prior to acquiring a treatment image ...
-
bioRxiv - Cell Biology 2024Quote: ... the buffer was switched to imaging media with either 1mM or 11mM glucose with treatment (CB-100: 5μg/mL, nidogen-2 (40ng/mL, Biotechne R&D Systems)) and incubated for 5 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatants were used with the Human Phospho-EphB4 DuoSet IC ELISA kit (#DYC4057-2, R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2024Quote: ... 2 µM parkin (either full-length or truncated parkin) were incubated in the presence of 10 µM UbVS (R&D Systems) in 30 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Developmental Biology 2020Quote: ... the membranes were incubated overnight at 4°C with anti-Gremlin1 antibody (R&D systems, AF956, 1:1000) or anti-βActin (Santa Cruz ...
-
bioRxiv - Neuroscience 2020Quote: ... The blots were incubated overnight at 4 °C with goat anti Dscam (AF3666, 1:500, R&D systems) and mouse anti-tubulin (12G10 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C with primary antibodies against pLCK (Y394) (1:1000) (R&D Systems), GAPDH (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... and incubated overnight at 4°C with antibodies against ACE2 (1:400; R&D systems, Minneapolis, MN, USA), TMPRSS2 (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cell pellet was stained for 1 hour at 4°C with primary ACE2 antibody (R&D systems #AF933 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated at 4 °C overnight with antibodies against TBXT/BRACHYURY (1/1000, R&D Systems: AF2085), SOX2 (1/1000 ...
-
bioRxiv - Bioengineering 2024Quote: Stage 1 (4 days): Base medium supplemented with 100 ng/mL Activin A (338-AC, R&D Systems) and 3 μM CHIR99021 (2520691 ...
-
bioRxiv - Cell Biology 2024Quote: ... Stage 1 (4 days): BE1 medium supplemented with 100 ng/mL Activin A (338-AC, R&D Systems) and 3 μM CHIR99021 (2520691 ...
-
bioRxiv - Developmental Biology 2021Quote: ... sheep anti-PAX6 (AF8150: R&D Systems, 5 µg/mL); rat anti-TBR2 (clone ...