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Citations for R&D Systems :
1051 - 1100 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... with 5 % fetal bovine serum (R&D systems, S11150). HEK 293T cells from ATCC were maintained in DMEM medium with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... human ACE2 (5 μg/mL; R&D Systems, MAB10823) with Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: Polystyrene microtiter plates were coated with 1 µg/ml recombinant SARS-CoV-2 spike protein produced in HEK293T cells (10549-CV-100, R&D Systems and Biotech, NE, MN, USA) while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Molecular Biology 2022Quote: ... and interleukin-4 (IL-4, 50 ng/mL, R&D Systems, 6507-IL-025/CF) for 5 days ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then cultured with bone morphogenic protein-4 (BMP-4; R&D Systems) and CHIR-99021 in RPMI-B27 medium to specify for cardiac mesoderm lineages ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4°C in the following primary antibodies: T-LCK (1:1000 R&D Systems), T-LCK (1:1000 Proteintech) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:200; Af114, R&D Systems). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were labeled with anti-human SSEA-4 APC-conjugated antibody (1:100, R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:50; Af114, R&D Systems). The next day ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... organoids were grown embedded in a mix of 90% ice-cold RGF BME Type 2 PathClear (Cultrex, R&D Systems) and 10% Dulbecco’s modified Eagle’s Medium/Ham’s F12 (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Bioengineering 2021Quote: ... Virus infection was detected with mouse mAb to SARS-CoV- 2 nucleocapsid (N) (R&D Systems, Minneapolis, MN, Cat#MAB10474) and counterstained with rabbit anti-mouse IgG-HRP (Rockland Immunochemicals ...
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bioRxiv - Developmental Biology 2022Quote: ... the differentiation media consisting of aRB27 with 2% Geltrex or Cultrex (+BME overlay) and 10ng/ml BMP4 (R&D Systems) was added to the cells [or with variations ...
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bioRxiv - Neuroscience 2023Quote: ... Culture media consists of N2B27 base medium supplemented with 20 ng/mL FGF-2 (R&D Systems, 233-FB-010), and 50 units/mL-50ug/mL Penicillin-Streptomycin (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... DIV12 hippocampal neurons were treated with either 2 ug/mL of recombinant Nptx2 (CF 7816-NP-050, R&D Systems) or BSA as control ...
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bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... A feeding layer of 2 ml RPMI growth medium supplemented with 10 ng/ml PDGF-BB ligand (R&D Systems) and/or 0.01 uM siRNA (see below ...
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2023Quote: ... The supernatant was analyzed using the mouse Lipocalin-2 enzyme-linked immunosorbent assay (ELISA) Duoset (DY1857-05, R&D Systems) according to the manufacturer’s instruction in serial dilutions.
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bioRxiv - Immunology 2023Quote: ... mouse TNF-alpha DuoSet ELISA (DY410) and DuoSet ELISA Ancillary Reagent Kit 2 (DY008) were purchased from R&D system.
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bioRxiv - Immunology 2024Quote: ... ELISA kits were used to quantified ferritin (Christal Chem) and soluble mouse CD25/IL-2 alpha receptor (R&D Systems) following manufactorers instructuon.
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tissue pellet was resuspended in cold Cultrex Reduced Growth Factor BME type 2 (Cultrex BME) (R&D systems) and 25 mL drops per well were plated on pre-warmed 48-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Brain homogenates were prepared by adding an equal volume of Cell Lysis Buffer 2 (R&D Systems, Minneapolis, MN, USA) to dissociated brain tissues ...
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bioRxiv - Developmental Biology 2021Quote: ... BMP4 (range tested: 4-160 ng/ml, R&D Systems), 100 ng/ml GDF7 (R&D Systems) ...
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bioRxiv - Biophysics 2020Quote: Recombinant human ADAMTS-4 (R&D Systems, Minneapolis, MN, USA) and MMP-13 (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... rhIL-4 (final concentration 200 ng/ml, R&D Systems) and CpG ODN2006 (final concentration 1 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... and goat anti-human TSP-4 (AF2390, R&D Systems) using Vecta Stain ABC Kit (PK-6104 ...
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bioRxiv - Cancer Biology 2020Quote: ... and goat anti-human TSP-4 (AF2390, R&D Systems). Sections of human tumors were stained with mouse anti-human CD68 (Dako ...
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bioRxiv - Immunology 2021Quote: ... 4 μg/mL of each Siglec-Fc (R&D Systems) was pre-complexed with either 8 μg/mL AffiniPure Donkey anti-human IgG Alexa Fluor 647 (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2021Quote: ... All recombinant factors were purchased from PeproTech with the exception of hBMP-4 from R&D Systems.
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bioRxiv - Neuroscience 2022Quote: ... IL-4 (20 ng/mL, 24 h, R&D Systems) or apoptotic SH-SY5Y neuroblastoma cells (2:1 ratio neurons:microglia ...
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bioRxiv - Developmental Biology 2023Quote: ... and BMP-4 (314-BP-010 Biotechne/R&D Systems) from 0.5-10ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Mouse IL-4 DuoSet ELISA (R&D Systems, catalog# DY404) and Mouse IL-13 DuoSet ELISA (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX; 10 μM; R&D Systems) was then added to determine the effect of AMPA receptor blockade on ePSCs ...
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bioRxiv - Microbiology 2022Quote: ... 100 uM of fluorogenic tripeptide substrate Methionine-Glycine-Proline-7-amido-methylcoumarin (R&D Systems ES017); and 2 ug/mL of MetAP enzyme (R&D Systems 3537-ZN) ...
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bioRxiv - Immunology 2022Quote: ... USA) and cultured for 7 days with 20 ng/ml recombinant M-CSF (R&D Systems) to allow them to differentiate to macrophages ...
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bioRxiv - Systems Biology 2023Quote: ... BMDMs were stimulated on day 7 with the appropriate doses of IL-6 (R&D Systems) and IL-10 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...