Labshake search
Citations for R&D Systems :
1051 - 1100 of 1144 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
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bioRxiv - Bioengineering 2021Quote: Conditioned media from each cell-line/passage (at the time of cell harvest) were collected and prepared for cytokine profiling using the Human Premixed Multi-Analyte Magnetic Luminex Assay (R&D Systems, Minnneapolis MN) (Supplemental Table 3) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell lines were purchased from ATCC (Manassas, VA, USA) and confirmed to be mycoplasma free by MycoProbe kit (R&D Systems, Minneapolis, MN, USA). Cells were grown at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The medium for differentiation consisted of DMEM/F12 supplemented with 5% R-Spondin1 conditioned medium (home made from Cultrex 293T R-spondin1-expressing cells, R&D Systems, MN, USA), 10% Noggin conditioned medium (home made with HEK293-mNoggin-Fc cells ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with PBS and stimulated with cytokine cocktail consisting of IL-1β (5 ng/ml, R&D Systems, Minneapolis, MN, USA), and IFN-γ (50 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: Twenty thousand HUVEC cells were seeded in reduced growth factor basement membrane extract (BME)-coated 96-well plate (R&D systems: 3470-096-K). Endothelial cells were exposed to 100 μl of the different benign or malignant cells conditioned media ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mullins’ laboratory (Voigt et al., 2019) at the University of Iowa and grown in endothelial cell growth medium (R&D Systems, Minneapolis, MN, USA). iCEC2 cells contain a temperature-sensitive hypomorphic T antigen and actively proliferate at 33°C ...
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bioRxiv - Cancer Biology 2022Quote: The levels of murine TRAIL/TNFSF10 (DY1121) in the cells were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (R&D Systems, Emoriville, California, USA). The OD value was measured by Microplate Reader (Multiskan Spectrum ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Cell Biology 2022Quote: ... MDA-MB-231 and HEK293T cells (ATCC) were cultured in DMEM (4.5 g/liter glucose, pH 7.2) with 5% FBS (Atlanta Biologicals/R&D Systems #S11150, Lot #F18071) 20 mM Hepes ...
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bioRxiv - Cancer Biology 2020Quote: ... were used for this study.1.8×106 22Rv1 cells stably transduced with doxycycline-inducible shScramble or shTMEFF2-9 shRNAs and mixed with Basement membrane extract (R&D systems, Minneapolis, MN) at a 1:1 ratio ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
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bioRxiv - Immunology 2020Quote: Secreted IL-1b and MCP-1 proteins in supernatants of monocytic cells stimulated with TNF-α were quantified using sandwich ELISA following the manufacturer’s instructions (R&D systems, Minneapolis, USA).
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bioRxiv - Immunology 2019Quote: ... human sputum supernatants or cell supernatants from in vitro experiments were assayed using commercial “duoset” enzyme-linked immunosorbent assay kits (R&D Systems, Abingdon, UK). The MesoScale Discovery (MSD ...
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bioRxiv - Neuroscience 2021Quote: Differentiated BMDM were plated in 96-well plate at density of 30,000 cells/well either in the presence of 40ng/ml M-CSF (R&D systems, 416-ML-050) or without M-CSF ...
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bioRxiv - Immunology 2021Quote: ... IL-1β and IL-33 levels in cell-free supernatant were measured by ELISA according to manufacturers’ instruction (all R&D Systems DuoSet ELISA). For in vivo infection ...
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bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.2 x 106 WT or Lyn KO Maver-1 cells or 1 x 106 primary CLL cells were seeded into the transwell upper inserts while media were supplemented with chemokine CCL19 (#361-MI-025, R&D Systems, Minneapolis, MN) at 200 ng/mL or 0.1% BSA in PBS (control ...
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bioRxiv - Neuroscience 2022Quote: ... we determined the amount of HB-EGF per cell through enzyme linked immunosorbent assay (ELISA; Cat# DY8239-05, R&D Systems, Minneapolis, MN, USA). For analysis of HB-EGF levels in brain tissue ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in appropriate cell culture medium as directed by supplier with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems; Minneapolis, MN) and 1% Penicillin-Streptomycin (Thermo Fisher ...
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Long Noncoding RNA MALAT1 is Dynamically Regulated in Leader Cells during Collective Cancer InvasionbioRxiv - Cancer Biology 2022Quote: A 3D invasion assay was applied to investigate the lncRNA dynamics in collective cancer invasion with Cultrex© 3D Spheroid Cell Invasion Assay Kits according to manufacturer’s instructions (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in the presence of 50 ng/mL of GM-CSF (02532, Stem Cells, Vancouver, Canada) and 50 ng/mL of M-CSF (216-MC, R&D Systems, Minneapolis, USA) at 37°C and 5% CO2 for differentiation ...
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bioRxiv - Immunology 2023Quote: ... Media was typically changed every 3 days to maintain cell viability and all supernatants were collected for enzyme-linked immunosorbent assay (ELISA) per kit instructions (R&D Systems, Minneapolis, MN) to quantify cytokine production ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Bioengineering 2022Quote: ... was determined using the dot blot-based mouse XL cytokine array kit for the model group breast tissues and human XL cytokine array kit and human XL oncology array kit for the human cell conditioned media following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... IP-10/CXCL10 release from mouse cells was assayed using the Quantikine Mouse IP-10 ELISA assay (R&D Systems, Catalog No. DY466-05) using cell-free supernatants of stimulated cells according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Cancer Biology 2022Quote: The concentration of PEDF was measured in the conditioned media collected from control and knockdown QGY-7703 cells by using ELISA kit (R&D Systems, Minnesota, USA) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Protein profiling of the secreted factors from all cell cultures was performed using the Proteome Profiler Human XL Cytokine array kit (R&D systems, Minneapolis, MN) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... non-adherent hematopoietic progenitors were pooled and plated at 7x104 cell/well (96-well) with complete media supplemented with 30 ng/mL M-CSF (R&D Systems, 416-ML). At 90% confluence ...
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bioRxiv - Genetics 2023Quote: ... the cells were first seeded in a 96-well plate at a density of 50,000 cells per well in RDM containing 10% FBS and 10 µM ROCK inhibitor (R&D Systems; Cat# Y-27632). On Day 2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Bioengineering 2023Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Molecular Biology 2024Quote: IL-1β and IL-10 amounts were measured in the cultured media of treated cells by commercially available ELISA kit as described by manufacture (Quantikine, R&D Systems, Abingdon, UK). Data were normalized to LPS and presented as percentage change in cytokines release ± SEM.
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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bioRxiv - Cell Biology 2024Quote: ... Seven different ligand conditions were tested at concentrations previously determined to elicit maximal cell responses49 (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM) ...
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bioRxiv - Cell Biology 2020Quote: ... dPSCs were differentiated into each of the three germ layers according to the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems®, Catalog # SC027B).
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
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bioRxiv - Physiology 2019Quote: ... Cells were washed with BEC medium containing 10% FBS and were re-suspended in Flow Cytometry Staining Buffer (1X) (R&D Systems, Minneapolis, MN, USA) supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...