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Citations for R&D Systems :
851 - 900 of 1144 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems) and StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR analysis of pluripotency markers was done using the Human Pluripotent Stem Cell Assessment Primer Pair Panel (R&D Systems, SC012) and amplification was done using GoTaq Green ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Molecular Biology 2020Quote: Cytokine levels in cell culture supernatants samples were determined using the Proteome Profiler Mouse Cytokine Array Panel A (R&D Systems) following the manufacturer recommendations.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in PBS and stained with a goat anti-human ACE2 polyclonal antibody (R&D Systems, Cat # AF933) or isotype goat polyclonal isotype control (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Canada.70 Cells were maintained in DMEM supplemented with 10% fetal bovine serum (TET tested, heat inactivated, R&D SYSTEMS INC), 1% antibiotic-antimycotic ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from CH12 cells cultured for 3 days with TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were activated in the presence of the following sets of cytokines and/or neutralizing antibodies (all from R&D Systems): Th0—media alone ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Neuroscience 2020Quote: ... Myeloid progenitor cells were treated in suspension for 1 h with 5μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were treated in the presence or absence of interferon γ (IFN-γ 25ng/ml (R&D Systems, 285-IF) for 24 hours ...
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bioRxiv - Genetics 2022Quote: Supernatants from transfected HEK293T cells were analyzed for their LBP content by Human LBP DuoSet ELISA (#DY870-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... During this time cells were cultured in MEF-conditioned medium (70% MEF-conditioned medium, 30% TSC medium, FGF4 (37.5 ng/ml, R&D System), Heparin (1.5 μg/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fixed cells were stained with 1:100 dilution of APC-conjugated anti-SOX-17 (R&D Systems, Minneapolis, MN, USA; IC1924A) for 30 mins at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were resuspended in an antibody solution containing 5μL DYKDDDDK Epitope Tag Alexa Fluor 647-conjugated Antibody (R&D Systems #IC8529R) and 45μL DBPS + 10% FBS and incubated in the dark at room temperature for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... cells were treated for 2 hours prior to infection with the IC50 concentration of anti-ACE2 antibody (R&D Systems: AF933) determined for the cell lines used ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 μl of cell culture media was treated and incubated with the Proteome profiler mouse cytokine array membrane (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).
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bioRxiv - Molecular Biology 2023Quote: ... Colony forming assays were performed by plating 200 cells of each isolated subpopulation in Complete Methylcellulose Media (R&D Systems; HSC003).
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bioRxiv - Cancer Biology 2023Quote: ... We used a Comet Assay Single Cell Gel Electrophoresis Assay kit (cat. no. 4250-050-K; R&D Systems, Bio-Techne) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFβ concentration in the cell free BALF was calculated using Mouse TGFβ 1 DuoSet Elisa (R&D Systems Cat DY1679-05) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Supernatants harvested from infected cells were assayed using enzyme-linked immunosorbent assay (ELISA) kits for human IL-18 (R&D Systems) and IL-1β (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells were pre-treated for 16 h with Type I Interferon (1000 Units/mL; R&D Systems, 11200-2) and treated with HMW Poly(I:C ...
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bioRxiv - Immunology 2023Quote: ... HUT78 cells were stimulated in complete IMDM media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems). All treatments were 24 hours.
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bioRxiv - Immunology 2023Quote: ... K562 cells were stimulated in complete RPMI media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems), 20 ng/mL human IFN-β (84-99I-F010 ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ levels were measured in NK cell media samples using the human IFN-γ DuoSet ELISA kit (R&D Systems). IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems) (53 ...
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at RT with anti-PAR/pADPr monoclonal antibody (R&D Systems cat# 4335-MC-100) diluted 1:250 in 3% BSA-PBS ...
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bioRxiv - Genomics 2023Quote: ... MDA-MB-231 cells were cultured in DMEM high-glucose medium with 10% FBS (Thermo Fisher Scientific or R&D Systems). Doxycycline-inducible GATA3 expression system in MDA-MB-231 cells was developed by lentiviral transduction ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad) ...
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bioRxiv - Genomics 2023Quote: ... Cells were seeded in 96-well plates and 24 hours after plating treated with: THBS1 (R&D systems, 7859-TH-050), syndecan-4 Ab (BD Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: ... Harvested CM was normalized to the fibroblast cell count and assessed for IGFBP2 using ELISA purchased from R&D system (DGB200). Serum samples were diluted 50-fold ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Fixable Viability Dye eFluor™ 780 (65-0865-14) and CD34 (RQR8)-APC (R&D Systems; FAB7227A), washed in PBS and resuspended in BD Cytofix/Cytoperm™ (BD ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... The cell mediums were collected and used to detect the TNFα levels by the TNFα ELISA kit (R&D Systems, MTA00B). All ELISA assays were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Biophysics 2023Quote: ... about 15.000 cells per well were resuspended in Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) (R&D Systems) and seeded in pre-heated low adhesion 24-well plates (GreinerBio) ...
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bioRxiv - Cancer Biology 2023Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5 × 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated with Accutase and plated again at 80,000 cells/ml in SFD supplemented with 75ng/ml Activin A (R&D Systems). After 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then incubated with a 5 µg/mL of human HNF-3 beta/FoxA2 antibody (R&D Systems; AF2400) for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: The levels of CXCL9 secreted to the media by MIN6 cells were quantified by Mouse DuoSet ELISA kit (R&D Systems). Polystyrene 96-well plates were coated with 100 μL of 200 ng/mL unconjugated goat anti-mouse CXCL9 antibody in PBS at room temperature overnight ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were isolated from the spleens of female OT-I (CD45.1+/CD45.2+) mice using CD3+ T cell enrichment columns (R&D Systems, MTCC25) and co-cultured with the peptide-pulsed splenocytes for 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Cell Biology 2020Quote: ... a second primary antibody was used to highlight non-differentiated cells on epithelia for 2h at room temperature: p63 (AF1916, R&D Systems) or pan-cytokeratin (CK ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...