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Citations for R&D Systems :
1051 - 1100 of 3428 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... naïve B cells or CH12F3 cells were treated with 10ng/ml F(ab’)2 Fragment Goat Anti-Mouse IgM or 10ng/ml mIL-5 (R&D Systems) for 5 or 10 min and then collected for Western blotting or RNA extraction.
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bioRxiv - Immunology 2023Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of rhuIL-2 (10 U/mL, R&D System) to expand the T cells ...
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... TSP2 concentrations in LX-2 cell lysates were determined using enzyme-linked immunosorbent assays following the manufacturer’s instructions (Quantikine® ELISA, #DTSP20, R&D Systems) (16) ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Immunology 2023Quote: ... Unpurified transient transfection supernatant samples (50 µl) were pre-incubated with biotinylated SARS-CoV-2 spike protein (R&D Systems cat# BT10549) (50 µl ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Bioengineering 2024Quote: ... Enzyme-linked immunosorbent assays (ELISA) was then carried out as per manufacturer’s instructions (R&D Systems, BMP-2 DuoSet ELISA kit, DY355). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 2 h at room temperature with secondary antibodies in TBS-T containing 10% milk: anti-Mouse IgG (HAF018, R&D Systems) and anti-Rabbit IgG (A9196 ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
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bioRxiv - Immunology 2022Quote: ... cells were washed and split into two new 48-well plate wells and cultured with IL-2 (5 ng/ml; R&D Systems), in the presence or the absence of CH-223191 (10 µM ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Immunology 2024Quote: Fecal samples collected from female mice at 5 weeks of age were assayed for immunoglobulin and Lcn-2 content using ELISA kits (Mabtech; R&D Systems). Protein was extracted from fecal samples by manual homogenization of 2-3 pellets in PBS + 0.05% Tween20 followed by 4 hr of agitation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated overnight at 4°C with a primary antibody specific for IL-1α (15 μg/mL in PBS with 2% BSA, AF-400 NA R&D Systems). After washing with Tris-buffered saline ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Cancer Biology 2024Quote: A pool of plasma from 2 to 4 mice was loaded on the membrane of the Proteome Profiler Mouse XL cytokine Array (ARY028, R&D systems) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... then maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems). For the pilot experiment in which CD4 cells with a defined CAR BC were spiked into CD8 cells (Figure 1D-F) ...
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bioRxiv - Immunology 2024Quote: ... All primary cells were maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems) at 37°C in 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... The neutralizing capacity of the αVEGF-B monoclonal antibody was evaluated by stimulating confluent HUVECs pre-starved for 2 h in GFD media with a fixed amount of VEGF-B186 (50 ng/ml; R&D Systems) for 2 h in GFD media ...
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bioRxiv - Plant Biology 2024Quote: In vitro E3 ubiquitin ligase assays were performed by incubating 2 μg of recombinant GST-MpPUB20e and 75 μg/mL of HA-tagged ubiquitin (R&D Systems) in a buffer containing 25 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cell Biology 2024Quote: ... were O/N coated at 4°C with 5 µg/ml Invasin protein in PBS or 2% BME® (R&D system) diluted in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... the level of the chemokine IL-8 in the basal medium of Caco-2/TC7 cells was quantified using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems).
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bioRxiv - Cell Biology 2024Quote: ... Spheroids were incubated in blocking buffer (3% BSA 1% Tween-20 in PBS) for 2 hours and then incubated with primary antibodies (goat anti-mouse VE-Cadherin (AF1002, R&D systems) (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... Goat anti-Dll4 (*with antigen retrieval 1:50 and without antigen retrieval 1:25, AF1389, R&D Systems), *Rabbit anti-NICD activated (1:75 ...
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bioRxiv - Neuroscience 2022Quote: ... 1% P/S and 0.l l⍰lμ gl⍰lml-1 of interleukin (IL)-34 (R&D Systems) and 0.01 μg ml −1 of granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were stained with TRA-1-60 conjugated FITC antibody (R&D system, FAB4770G, 1:50 dilution) or normal IgM conjugated FITC (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies were as follows: goat anti-Lyve-1 (R&D Systems, Minneapolis, MN, USA; 1:600), rabbit anti-Prox-1 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Chicken anti–GFP (GFP10-20; AVES, 1:2000) Goat anti–mouse podocalyxin (AF1556; R&D Systems, 1:1000) Rabbit anti–mouse LYVE-1 (11-034 ...
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bioRxiv - Microbiology 2022Quote: ... we changed the RSPO-1 conditioned media to lyophilized RSPO-1 (80ng/ml) (R&D systems 4645-RS).
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bioRxiv - Microbiology 2021Quote: ... cells were blocked with 1% BSA and stained overnight with goat anti-ACE2 (1:200, R&D Systems) in blocking buffer followed by rabbit anti-goat Alexa Fluor 488 (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... then incubated with 1:1000 α-polySia mAb 735 (BioAspect) or 1:2000 α-NCAM (R&D Systems) in blocking buffer at room temperature overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... ELISA for HO-1 was performed using a human HO-1 DuoSet IC kit from R&D Systems, and total protein was assessed using a PierceTM BCA Protein Assay Kit.
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies (Rabbit anti-MAP2, Millipore, AB5622, 1:800; Mouse anti-Tuj1, R&D Systems, mab1195, 1:500) were diluted in PBS and added to the wells to incubate overnight at 4°C while gently rocking ...
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bioRxiv - Biophysics 2023Quote: ... The samples were then exposed to either 10 nM thrombospondin-1 (TSP-1) (3074-TH, R&D Systems) or HBSS for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg/mL bFGF (R&D Systems) and 50 IU/mL heparin (B ...
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bioRxiv - Cell Biology 2020Quote: ... MET (#AF276, 1:100, R&D Systems), EEA1 (#610457 ...
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bioRxiv - Physiology 2020Quote: ... HspB8 (R&D Systems, MAB4987, 1:800), Hsp70 (Proteintech ...
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bioRxiv - Cell Biology 2020Quote: ... FABP4 (R&D Systems, AF1443, 1:1,000). After washing ...
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bioRxiv - Developmental Biology 2021Quote: ... AXIN2 (1:200; R&D Systems, MAB6078), PDPN (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... PDPN (1:200; R&D Systems, AF3670), CD31 (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... SOX17 (1:100, R&D Systems AF1924); phosphorylated SMAD1/5/9 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... -MOG (1:80, R&D Systems, AF2439), -MBP (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... SOX2 1:300 (R&D systems, AF2018), H3.X/Y 1:200 (Active Motif ...
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bioRxiv - Genetics 2020Quote: ... 1 ng/mL IL3 (R&D Systems), and 2 U/mL EPO (Amgen) ...