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Citations for R&D Systems :
51 - 100 of 174 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... plates were incubated with 25 µl of a biotinylated anti-TREM2 antibody (R&D systems, BAF1729) at 0.125 µg/ml for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated with 3,3’,5,5’-tetramethylbenzidine according to manufacturer instructions (DuoSetELISA Development System, R&D Systems) and absorbance was read at 450 nm (VICTOR3 spectrophotometer ...
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bioRxiv - Immunology 2021Quote: ... Tissue culture 96-well plates were coated with DL4-Fc and VCAM-1-Fc (R&D Systems) overnight at 4°C or for 2-4 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... coated 6 well plates in oRIM supplemented with 2.6 nM (20 ng/mL) IGF1 (R&D Systems). On day 24 ...
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bioRxiv - Immunology 2023Quote: ELISA plates were coated with 2 µg/ml mouse anti-His tag antibody (R&D Systems, MAB050) and blocked as described above ...
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bioRxiv - Microbiology 2023Quote: ... ligand-loaded CD1d (0.25 ug per well) was bound onto Streptavidin-coated plates (R&D Systems #CP003). Plates were washed with PBS three times and then added 24.7 NKT cells (4 x 104∼105 per well ...
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bioRxiv - Immunology 2024Quote: Plates were centrifuged at 825xg for 5 min and supernatants collected for IFNγ ELISA (R&D Systems Mouse IFNγ DuoSet ELISA Kit ...
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bioRxiv - Immunology 2021Quote: ... PVDF backed microplate plates pre-coated with IFNγ-specific antibodies (R&D systems, mouse IFNγ Kit Cat # EL485) were washed with PBS and blocked with RPMI medium (Corning ...
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bioRxiv - Immunology 2022Quote: ... Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... were seeded on 6 well plate and cultured with recombinant mouse IFNγ (100 units/mL; R&D Systems) and recombinant human IL-1β (5 units/mL ...
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bioRxiv - Neuroscience 2021Quote: ... coated plates in N4 media (N3 with 0.05uM Retinoic acid, 2ng/ml BDNF (R&D Systems #248-BDB) and 2 ng/ul GDNF (R&D Systems #212-GD)) ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were incubated with 0.25 μg/ml biotinylated sheep anti-mouse TREM2 antibody (R&D Systems, BAF1729) for 1 hr at RT with shaking ...
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bioRxiv - Neuroscience 2022Quote: ... high-affinity binding 96 well plates were coated with a NOTCH3 capture monoclonal antibody (MAB1559; R&D Systems) at 0.625 ng/µL in 100 µL of PBS and agitated overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were transferred to 96-well plates and treated with 20ng/ml of IFN-γ (R&D Systems) for 48 hours prior to co-culture with T cells ...
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with anti-mouse protein C polyclonal sheep IgG (R&D Systems, Minneapolis, MN) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: 96-well plate was coated overnight at 4 °C with TREM2 coating antibody (MAB17291-100, R&D Systems) in ELISA Coating Buffer (421701 ...
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bioRxiv - Developmental Biology 2024Quote: ... cell culture plates were pre-coated with recombinant human DLL1- IgG1-Fc (Biotechne R&D systems, 10184-DL), human JAG1-IgG1-Fc (Biotechne R&D systems ...
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bioRxiv - Cell Biology 2022Quote: ... On the day of the measurements media from the plate was aspirated and Resazurin reagent (R&D Systems, AR002) diluted 1:10 in media was added to each well ...
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bioRxiv - Biochemistry 2021Quote: ... 16055 NFL trimers were captured on the ELISA plate by a mouse anti-His tag mAb (R&D Systems) coated at 2 μg/ml in PBS at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ELISA was performed using the Solid Phase Sandwich Quantikine ELISA Kit (96-well strip plates, R&D Systems, Canada) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: For TCR-mediated adhesion 96-well plates were pre-coated with 0.5μg human ICAM-1/well (R&D systems). Transfected cells were left untreated or stimulated with anti-TCR antibody (OKT3 ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated to streptavidin (R&D Systems), diluted 40× in T-PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Laminin was added to plate in 50 ul of base media (DMEM/F12 supplemented with N2 (R&D Systems), B-27 (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... Plates used for virus production are rinsed briefly with poly-L-Lysine (R&D Systems Cat#3438-200-01). 293T/17 are seeded at 110k/cm2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then plated in duplicates at 3,000 viable cells/plate using MethoCult methylcellulose media (R&D Systems, HSC007). After 14 days ...
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bioRxiv - Cancer Biology 2024Quote: ... A 96-well ELISA plate was coated overnight with TREM2 capture antibody (R&D Systems; cat. #MAB17291; RRID: AB_2208679). The plate was washed 3 times ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems, UK). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Biochemistry 2023Quote: ... were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37°C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM ...
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bioRxiv - Cancer Biology 2023Quote: 10^4 LECs were seeded on 96-well plates pre-coated with 50 μl of Cultrex (3433, R&D Systems) and incubated 24 h in the cell incubator ...
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bioRxiv - Physiology 2024Quote: ... 24 well-plates were coated with recombinant murine (rm) E-selectin (rmCD62E-Fc chimera, 10µg mL-1, R&D Systems) or PBS/0.1% BSA at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Nunc® Maxisorp™ 384-well plates were coated with 6 µg/ml of recombinant mouse pIgR protein (R&D Systems) diluted in coating solution concentrate (Seracare ...
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bioRxiv - Neuroscience 2020Quote: ... onto Matrigel-coated plates in E8 Flex medium plus 10 μM ROCK inhibitor Y-27632 (R&D Systems, cat no. 1254). The next day ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Developmental Biology 2021Quote: ... MSD standard-bind microtitre plates were first coated with 30µl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 µg/ml in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were seeded on Matrigel coated culture plates in mTeSR supplemented with 10µM Y-27632 dihydrochloride (R&D Systems, 1254/10) at a density of 56,000/cm2 for routine maintenance of iPSC lines ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems, UK). Plates were left to incubate at room temperature for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... the endodermal cells were dissociated into single cells using Accutase and plated on the Matrigel-coated plates in serum-free differentiation media (SFM) supplemented with 1.5 μM Dorsomorphin dihydrochloride (R&D System) and 10 μM SB431542 for 24h ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... A 384-well maxisorp plate (Thermo, Cat# 464718) was coated overnight with an anti-IDS antibody (R&D Systems, Cat# AF2449) and blocked with Casein-PBS Buffer (Thermo ...
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bioRxiv - Neuroscience 2021Quote: For sTREM2 measurements, multi array 96-well plates (MSD, L15XA-3) were coated with mouse anti-TREM2 (R&D systems, AF1729) capture antibody O/N at 4°C ...
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bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: ... CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well) in the presence of 20 ng/mL M-CSF (216-MC-005; R&D Systems) for 4 days.6 Monocyte-derived macrophages [(Mb) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed twice with TBS/0.05% Tween20 before incubating with serially diluted mAbs and CD81-LEL-hFc (R&D systems) in casein blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Immunology 2023Quote: ... A microtiter plates with 96 well were incubated with anti-TNF-α or anti-IL-1β primary antibodies (R&D Systems) diluted in PBS (1:1000) ...