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Citations for R&D Systems :
1 - 50 of 174 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... U-bottom 96 well plates in Essential 8 medium supplemented with 50 ng/mL BMP-4 (R&D Systems, 314-BP010) and 10 μM Rock inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Microbiology 2021Quote: CCL22/Ccl22 ELISAs were performed on culture supernatants (undiluted or diluted when necessary) using Immulon 4HBX flat bottom microtiter plates with the human CCL22/MDC DuoSet ELISA (R&D Systems DY336) or the mouse Ccl22/Mdc DuoSet ELISA (R&D Systems DY439 ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Immunology 2022Quote: ... NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL rhIL-2 (R&D Systems, Cat. 202-IL-010). NK cells were then placed into a 37°C incubator ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... The remaining NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL (250 IU/mL) rhIL-2 (R&D Systems, cat. no. 202-IL-010), then placed in a 37C CO2 incubator for 12-16 hours ...
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bioRxiv - Bioengineering 2022Quote: ... The bottom rings were spotted with antibodies to IL-1β (R&D Systems) at 500 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Microtiter plates (R&D Systems) were first coated with a monoclonal rat antimouse IgE (clone R35–72 ...
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bioRxiv - Cancer Biology 2021Quote: ... bottom chambers were filled with 500 μL of serum-free DMEM with chemoattractants (R&D Systems). Cells were cultured overnight at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: Quantikine ELISA plates (R&D Systems, UK) came pre-coated with capture antibody ...
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bioRxiv - Immunology 2023Quote: ... 5μg/ml plate-bound (R&D Systems); Orlistat ...
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bioRxiv - Neuroscience 2020Quote: ... on Poly-D-lysine and laminin coated glass coverslips (Sigma-Aldrich, R&D Systems, and Academy Science Limited ...
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bioRxiv - Immunology 2022Quote: ... A 96-well ELISA plate (R&D system) was coated with the recombinant RBD protein (Acro Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... gas-permeable ELISA plate sealers (R&D Systems). Plates were read in Epoch Plate Reader (BioTek ...
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bioRxiv - Biochemistry 2022Quote: ... 8-chamber glass slides were coated with 5μg/ml vitronectin (R&D systems, 2308-VN) for 2 hours at room temperature prior to being seeded with parental RPE-1 cells ...
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bioRxiv - Neuroscience 2020Quote: ... Glass coverslips were coated with alternating stripes (IgG or Sema7a 100 μg/ml, R&D Systems), which were then covered by laminin (20 μg/ml) ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with anti-hACE2 (R&D Systems) diluted in 1% non-fat milk PBS 0.05% Tween 20 for 2 h at 37°C ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Systems Biology 2022Quote: Pre-coated ELISA plates were purchased from R&D Systems and protocols were performed according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and HRP-streptavidin 1000 (R&D Systems) diluted and were added in each well ...
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bioRxiv - Neuroscience 2021Quote: ... The sandwich layout of this TREM2 MSD assay from the bottom to the top is (1) Capture-antibody (1 μg/mL; BAF1729, R&D Systems), (2 ...
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bioRxiv - Cell Biology 2022Quote: Glass coverslips (#1.5, 12mm diameter) were coated with Cultrex Poly-L-Lysine (R&D systems, 34-382-0001) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... a DuoSet ELISA plate (R&D Systems Inc, Minneapolis, MN, USA) for the detection of IFN-β was prepared following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... RBD was coated onto a 96-well ELISA plate (R&D system) at two ng/well in coating buffer (15 mM sodium carbonate ...
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bioRxiv - Bioengineering 2020Quote: ... Albumin was coated onto a 96-well ELISA plate (R&D system) at 1-10 ng/well in coating buffer (15 mM sodium carbonate ...
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bioRxiv - Immunology 2022Quote: ... cell culture plates were coated with recombinant DLL4-FC (R&D systems) overnight at 4°C at 0.5 μg/mL prior to the addition of FLS and cytokines ...
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bioRxiv - Pathology 2021Quote: ... and grown on Cultrex coated plates (R&D Systems, 3434-010-02). Cells were routinely passaged using our automated platform in the presence of Accutase (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 96-well microtiter plates were incubated with primary antibodies (R&D Systems) diluted in PBS (1:1000) ...
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bioRxiv - Microbiology 2020Quote: ... BHK21-hACE2 or the control BHK21 cells seeded onto fibronectin-coated glass coverslips were incubated with 0.4 μg/mL of hACE2-specific goat antibody (#AF933, R&D systems) at 4°C in media containing 25 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... glass coverslips were sterilized in 70% EtOH and coated with 10 µg/ml fibronectin (R&D Systems, Minneapolis, Minnesota, #1030-FN) in PBS for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to an IL-17A ELISpot plate (R&D systems) for an additional 24h and developed according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The plate was read using the Luminex Magpix analyser (R&D systems, USA).
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bioRxiv - Microbiology 2022Quote: Cover glass (FisherBrand, 12-546-P) was sterilized in 70% EtOH and coated with BME Cultrex (R&D Systems, 3432-010-01) prior to seeding ...
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bioRxiv - Developmental Biology 2024Quote: ... All of the experiments with MEFs were performed at passage 4 (P4) and at 60-70% confluence on glass fibronectin (1030-FN-05M, R&D Systems) coated coverslips or plastic culture plates (Starlab).
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bioRxiv - Neuroscience 2021Quote: ... the plates were precoated with anti-TRKB antibody (1:1000, R&D Systems, #AF1494) in carbonate buffer (pH 9.8 ...
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bioRxiv - Bioengineering 2022Quote: ... plates were incubated with respective detection antibodies diluted in reagent diluent (R&D systems) (anti-Apis mellifera defensin-1 ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound CD155-Fc chimera protein (CD155-IEGRMDP-Mouse IgG2a; R&D Systems). For plate coating with Abs or Fc ...
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bioRxiv - Physiology 2023Quote: ... before plates were incubated with 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution (R&D Systems). Enzymatic reactions were quantified by measuring absorbance at 450 nm using a standard plate reader (Biotek Instrument).
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bioRxiv - Cancer Biology 2021Quote: ... supernatant was transferred onto CXCL10 enzyme-linked immunosorbent assay (ELISA) plates (R&D Systems DY466) using the JANUS G3 MDT automated workstation (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... plates were blocked in 300 ml of 1x reagent diluent concentrate 2 (R&D Systems) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... the plate was washed (0.05% Tween 20 in PBS) and blocked (R&D system, DY995) at room temperature using the reagent diluent for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Cyto C Immunoassay 96-well plates followed by ELISA detection (R&D Systems, Minneapolis, MN). The fractional release of mitochondrial cytochrome c was calculated for each condition as [mean intensitysupernatant/(mean intensitymitochondria + mean intensitysupernatant)] ...
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bioRxiv - Neuroscience 2021Quote: ... coated plates in the expansion medium (N2B27) supplemented with 3 µM CHIR (R&D System, 4423), 0.5 µM PMA (Cayman Chemical ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK 293T cells were seeded on plates coated with Cultrex Poly-L-Lysine (R&D Systems) prior to transfection with the pCCl-c-MNDU3 STEAP1-BBζ CAR lentiviral plasmid and the packaging plasmids pMDL ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transferred to a new plate with fresh medium and IL-23 (R&D Systems). After 48 hours rest ...
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bioRxiv - Cell Biology 2022Quote: Plates (96-wells) were pre-coated with murine recombinant MadCAM-1 (R&D Systems, Minneapolis, MN.), fibronectin ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with biotin-linked anti-IL-1β detection antibody (R&D Systems, 1:1000), followed by horseradish peroxidase-conjugated streptavidin ...
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bioRxiv - Neuroscience 2024Quote: ... coated plates in the expansion medium (N2B27) supplemented with 3 µM CHIR (R&D System, 4423), 0.5 µM PMA (Cayman Chemical ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed five times and incubated with mouse TREM2 biotinylated antibody (R&D Systems, BAF1729) for 2 h at RT with constant shaking ...