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Citations for R&D Systems :
51 - 100 of 2977 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated using a primary antibody against NPHS1 (R&D Systems, #AF4269), diluted 1:100 in FACS buffer (PBS supplemented with 1% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... was determined using the dot blot-based mouse XL cytokine array kit for the model group breast tissues and human XL cytokine array kit and human XL oncology array kit for the human cell conditioned media following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Biochemistry 2021Quote: ... cells were resuspended in Goat anti-Human ACE2 antibody (1:500, R&D Systems) in the flow buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with recombinant human IFNγ (R&D Systems, 285-IF-100) by adding an equal volume of the complete culture media containing the cytokine ...
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bioRxiv - Genomics 2019Quote: ... 50 ng/mL carrier-free recombinant human stem cell factor (SCF; R&D Systems), 50 ng/mL TPO (R&D Systems) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 100ng/mL human recombinant FGF4 (R&D Systems, 235-F4) in the presence of 10 μg/mL heparin sulfate and 3 μM BI-D1870 RSK inhibitor at 37°C ...
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bioRxiv - Immunology 2022Quote: ... cells were immunostained with a goat anti-human ACE2 antibody (#AF933, R&D Systems) followed by immunostaining with a secondary antibody donkey anti-goat Alexa Fluor 488 (#A-11055 ...
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bioRxiv - Systems Biology 2019Quote: ... cells were incubated with titrated concentrations of human TGF-β1 protein (R&D systems) in the complete culture medium ...
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bioRxiv - Microbiology 2020Quote: ... cells were then fed with 20ng/ml human BMP4 (R&D Systems, PRD314-10), 0.5µM all-trans retinoic acid (ATRA ...
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bioRxiv - Developmental Biology 2022Quote: ... the dissociated cells were stained with APC-conjugated anti-human DLK1 (R&D systems). Cells stained with the cell surface marker and those expressing NT were sorted using FACSAria Fusion (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stimulated with 50 ng/ml recombinant human BMP-9 (R&D Systems) and 150 ng/ml recombinant human VEGF (PeproTech ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... the cells were treated with Recombinant Human TNFα Protein (R&D Systems, MN, USA) at 25ng/mL for 18 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight with human PRL (R&D Systems, 682-PL-050)
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 10μg/ml human anti-TNFR1 (clone 16805) (R&D System), 20μg/ml human anti-IFNGR1 (clone GIR208 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with mouse anti-human CCR1 antibody (2.5 µg/106 cells; mab145, R&D Systems) for 20 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with anti-MHC primary antibody (MHC; R&D Systems, Minneapolis, MN, USA) (1:400 ...
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bioRxiv - Immunology 2022Quote: ... Primary B cell complete media also contains 5 ng/ml of BAFF (R&D Systems).
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bioRxiv - Genomics 2021Quote: ... Cells were then incubated in primary antibodies (SOX17-R&D Systems, AF1924, SOX2-Millipore, ab5603) diluted at 1:500 in 2% horse serum ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2020Quote: ... cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma ...
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bioRxiv - Cell Biology 2022Quote: Cells were characterised using hPSC imaging kit (Human Pluripotent Stem Cell Marker Antibody Panel kit, SC008, R&D system, UK) with analysis performed on a flow cytometer (Beckman Coulter Cytomics FC 500) ...
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bioRxiv - Molecular Biology 2020Quote: Mice fibroblast cells (FLS) treated with 5 ng/mL recombinant human TNF (R&D Systems) for 16 hours were harvested at 95% confluence ...
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bioRxiv - Immunology 2020Quote: ... isolated NK cells were cultured in 1000 IU/mL human IL-2 (R&D Systems) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated in SFD medium supplemented with 100ng/ml human Noggin (R&D Systems) and 10μM SB431542 for 24 hours followed by switching to SFD media supplemented with 10 μM SB431542 and 1 μM IWP2 (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were treated with 5 ng/mL recombinant human TGFβ1 (R&D Systems, 240-B) prepared according to the manufacture’s specifications ...
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bioRxiv - Bioengineering 2023Quote: ... as well as the Human Mesenchymal Stem Cells Multi-Color Flow Kit (R&D Systems) including antibodies for positive markers CD44 ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with 25 ng/ml human IL-17 recombinant protein (R&D Systems). Vehicle control or 25 ng/ml human TNF-α recombinant protein were used as controls ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with 1 ng/mL of recombinant human TGF-β1 (R&D Systems), 10 ng/mL of recombinant mouse IL-4 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... recombinant human PD-L1 human IgG1 Fc chimera protein (R&D Systems), or mouse IgG1 isotype antibodies (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... human fibronectin and human recombinant BMP-2 (R&D systems, Biotechne, UK); recombinant human BMP-2 (Infuse/InductOS® Bone graft kit ...
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Neuroscience 2020Quote: ... cells were preincubated for 10min with 30µg rat anti-human neuropilin1 Fc chimera (R&D System) or 20µg goat anti-human neuropilin2 Fc chimera (R&D System ...
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bioRxiv - Genomics 2022Quote: ... cells were incubated with indicated concentrations (Figure 1B) of human TGF-β1 protein (R&D systems) in the complete culture medium ...
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bioRxiv - Cell Biology 2022Quote: ... cells were exposed for 2 days to human Activin A (8 ng/ml, R&D Systems) and human bone morphogenetic protein 4 (hBMP4 ...
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bioRxiv - Cancer Biology 2022Quote: Medium from cells was harvested and used in the Human Cytokine Array Kit (R&D Systems), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and/or cells were activated with recombinant human IL-12 and IL-18 (R&D Systems), or IL-2 and IL-33 ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Cells were co-stained with a rabbit anti-human VE-Cadherin (1:100, R&D Systems). Secondary antibodies included Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 546 donkey anti-rabbit IgG (both 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with recombinant human IL1β (208-IL-010, R&D Systems, Minneapolis, MN, USA) for 30 min before immunofluorescence ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with either BSA or human recombinant WNT3a (5036-WN-010, R&D Systems) 24 hours later ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were stained with Human CD46 Alexa Fluor 594-conjugated Antibody (R&D systems FAB2005T-1). Alexa Fluor 594 signal was detected with a 561 nm yellow laser and a 585/16 bandpass emission filter ...
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bioRxiv - Bioengineering 2023Quote: P4 MSCs were assessed using the Human Mesenchymal Stem Cell Functional Identification Kit (R&D System) following the product instruction ...
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bioRxiv - Bioengineering 2023Quote: P4 MSCs were characterized with the Human Mesenchymal Stem Cell Verification Flow Kit (R&D Systems), including antibodies for positive markers CD90 ...
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bioRxiv - Cell Biology 2024Quote: ... The Phase I expansion media contains 50 ng/mL human stem cell factor (R&D Systems), 10 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... Primary keratinocytes or HEK 293T cells were labelled with nectin-4PE antibody (clone 337516; R&D Systems) to assess the expression of nectin-4 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with a primary antibody against ACE2 (4µg/ml, R&D Systems, catalog no. AF933) followed by a secondary antibody labelled with Alexa Fluor 568 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a primary antibody against p65 (1:25; R&D systems, Wiesbaden, Germany) for 1 h followed by the incubation with a Cy3-conjugated secondary antibody (1:250 ...