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Citations for R&D Systems :
351 - 400 of 2812 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Harvested supernatants from infected human macrophages were assayed for cytokine levels using ELISA kits for human IL-1α (R&D Systems), IL-18 (R&D Systems) ...
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bioRxiv - Bioengineering 2023Quote: ... Human IL-2 in the supernatant was measured using a sandwich ELISA kit (Human IL-2 Quantikine ELISA, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Single human recombinant DUBs (800 nM) were added together with 50 μM human recombinant HA-tagged ubiquitin (R&D SYSTEMS, U110) to each reaction ...
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bioRxiv - Bioengineering 2021Quote: ... Kidney tissue sections were incubated with 1μg/mL of mouse-α-Tubulin primary antibody and Goat-α-CD31 primary antibody (R&D Systems, AF3628) in 0.1% Triton-X/PBS at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: goat anti-VEGFR3 (R&D Systems), rabbit anti-PROX1 (AngioBio) ...
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bioRxiv - Genomics 2019Quote: ... incubated with anti-nephrin primary antibody (R&D Systems, AF4269) and APC-conjugated anti-podocalyxin antibodies (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... with primary antibody (sheep anti-THP, R&D Systems AF5144) in PBS + 2% BSA + 0.1 % Triton X-100 and secondary antibody + FITC-phalloidin (Molecular Probes ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-CD9 primary antibody (MAB1880) was from R&D systems, anti-CD81 (TAPA-1 ...
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bioRxiv - Cancer Biology 2021Quote: Primary antibodies were p16INK4a (AF5779, R&D Systems, 1:200), MDM2 (A2716 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies: E-Cadherin (1:500, R&D Systems, AF748), cleaved Caspase 3 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies: Polyclonal Sheep anti-Mouse PEAR1 (R&D Systems, Cat # AF7607 ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies (anti-Cd31, goat polyclonal, R&D Systems #AF3628 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies: Olig2 (Goat, 1:200, R&D Systems, AF2418), MBP (rat ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies recognizing PDGFRB (AF385, R&D Systems, 1:200), CD34 (AF7227 ...
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bioRxiv - Genetics 2023Quote: ... labeled with primary antibodies anti-SOX21 (AF3538, R&D Systems), anti-DCT (ab74073 ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies used: anti-PDGFRα (R&D systems, Cat.# AF1062), anti-EpCAM (Abcam ...
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bioRxiv - Biophysics 2023Quote: ... The primary antibody (goat@MET, #AF276, R&D Systems, USA) was diluted in BB to a final concentration of 2 µg/mL and incubated for 2 h at room temperature with gentle shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... Phospho-kinase arrays: proteome profiler human phospho-kinase array and human phospho-RTK array kits including 49 RTKs were purchased from R&D Systems. Indicated SW620 cells were lysed ...
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bioRxiv - Neuroscience 2021Quote: The cytoplasmic fraction of human postmortem brain homogenates and astrocyte culture media were used to determine cytokine content using Mouse or Human Proteome Profiler arrays (Mouse Cytokine Array Panel A and Human Cytokine Array, R&D Systems), according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine profiling of these pooled supernatants was performed using a human cytokine array kit (human cytokine array kit, panel A; R&D Systems) and near-infrared fluorescence detection using IRDye 800CW Streptavidin (Li-Cor Biosciences).
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bioRxiv - Bioengineering 2021Quote: ... and 100 U/mL Penicillin/Streptomycin supplemented with 0.1% recombinant human Fetal Growth Factor b (Alamone F-170) and 0.1% recombinant human Epidermal Growth Factor (R&D systems 236-EG). On Day 12 ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human ACE2 polyclonal antibody (AF933) and mouse anti-human ACE2 mAb MAB9332 were available from R&D Systems (Minneapolis, MA). Rabbit anti-SARS-CoV-2 RBD (40592-T62 ...
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bioRxiv - Immunology 2022Quote: ... Human CSF-1 and IL-34 human serum levels were quantified using ELISA kits (both R&D systems, Mountain View, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Neuroscience 2020Quote: ... Recombinant proteins were separately preincubated with neuronal cultures and recombinant tau protein for 3 hours prior to incubation of neuronal cultures with the mixer containing recombinant proteins (100nM Human LRP-1 Cluster IV Fc Chimera Protein; 10nM Human LRPAP Protein [R&D systems]). For pharmacological treatments ...
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Immunology 2019Quote: ... Immature dendritic cells were prepared by treatment of monocytes with 500 U/ml human IL-4 and 800 U/ml human GM-CSF (R&D Systems) in CellGenix media for 6 days ...
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bioRxiv - Cancer Biology 2020Quote: Human S100A8/S100A9 heterodimer amounts in human plasma samples were quantified using S100A8/S100A9 heterodimer Quantikine ELISA kit (R&D System, DS8900) following the manufacture protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Human 26S Proteasome Protein (E-365) and Human 20S Proteasome Protein (E-360) were purchased from R&D Systems (Minneapolis, MN, USA). Human KRAS (G12D ...
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bioRxiv - Neuroscience 2023Quote: ... Relative expression level of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: The relative cytokine content of the aged iCMs before and after ECM treatment and of the decellularized human heart ECM were obtained using the Human XL Cytokine Array Kit (ARY022B, R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Proteome Profiler Array Human XL Oncology Array (Catalog Number ARY026) and Human XL Cytokine Array (Catalog Number ARY022B) from R&D Systems, Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The culture supernatants were analyzed for cytokines using the Human TNF-α and Human IL-12 DuoSet ELISA kits (R&D Systems) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... Relative expression levels of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cancer Biology 2020Quote: ... Then we collected the supernatants and cell lysates so that we could detect the chemokines using a Proteome Profiler Human Chemokine Array Kit (R&D Systems, ARY017).
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
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bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The receptor tyrosine kinase (RTK) activities in cells after drug treatment were analyzed by a Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, ARY001B) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... were assessed from the culture supernatant of the activated CD4+ T cells by Human Th9/Th17/Th22 Luminex Performance Assay 17-plex Fixed Panel (R&D systems, USA). The assay was performed according to manufacturers instructions and samples diluted 1:2 with calibrator diluent ...
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bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Immunology 2023Quote: IFN-γ secreted in the supernatant after 72h of co-culture of tumor and effector T cells was measured using the Human IFN-gamma DuoSet ELISA kit (R&D Systems, DY285B) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Phospho-RTK Array Kit (ARY001B, R&D Systems) was used according to the manufacturer’s instructions and the blots were imaged and quantified in the same way as western blots.
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bioRxiv - Bioengineering 2021Quote: ... and anti-human PDGFR-β (R&D Systems, AF385). After three 8-hour washes in PBST at 4°C ...