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9251 - 9300 of 10000+ citations since 2019

• Inducible mechanisms of disease tolerance provide an alternative strategy of acquired immunity to malaria

  Wiebke Nahrendorf, et al., bioRxiv - Immunology 2020

  Quote: ... We used commercially available ELISA kits to quantify plasma IFNγ (mouse IFNγ platinum ELISA extra sensitive, ebioscience), CXCL10 (mouse IP-10 platinum ELISA, ebioscience) and Angiopoietin-2 (mouse/rat Angiopoietin-2 quantine ELISA kit, R&D Systems). Absorbance was measured using a Multiskan Ascent (MTX Lab systems ...
• Prostacyclin Promotes Degenerative Pathology in a Model of Alzheimer’s disease

  Tasha R. Womack, et al., bioRxiv - Neuroscience 2020

  Quote: The primary antibodies used were Collagen IV (1:400 dilution; Cosmo Bio, Catalog Number LSL-LB1403) and CD13 (1:100 dilution; R&D Systems, Catalog Number AF2335). Mice were sacrificed by decapitation ...
• Changing the Waddington landscape to control mesendoderm competence

  James R. Valcourt, et al., bioRxiv - Developmental Biology 2020

  Quote: ... SOX17 (1:100, R&D Systems AF1924); phosphorylated SMAD1/5/9 (1:200 ...
• Changing the Waddington landscape to control mesendoderm competence

  James R. Valcourt, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and NANOG (1:500, R&D Systems AF1997). The PAX6 antibody was deposited to the DSHB by Kawakami ...
• A TgfβRI/Snai1-dependent developmental module at the core of vertebrate axial elongation

  André Dias, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Primary antibodies (1:200): goat anti-Brachyury (R&D Systems, AF2085), rabbit anti-Sox2 (Abcam ...
• A TgfβRI/Snai1-dependent developmental module at the core of vertebrate axial elongation

  André Dias, et al., bioRxiv - Developmental Biology 2020

  Quote: ... goat anti-Tbx6 (R&D Systems, AF4744), rabbit anti-Laminin 111 which detects all laminins containing a1 ...
• A TgfβRI/Snai1-dependent developmental module at the core of vertebrate axial elongation

  André Dias, et al., bioRxiv - Developmental Biology 2020

  Quote: ... goat anti-human EpCAM/TROP1 (R&D Systems, AF960) and rabbit anti-Vimentin (Abcam ...
• RASA1-driven cellular export of collagen IV is required for the development of lymphovenous and venous valves in mice

  Di Chen, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Primary antibodies used were: goat anti-VEGFR3 (R&D Systems), rabbit anti-PROX1 (AngioBio) ...
• Base-resolution models of transcription factor binding reveal soft motif syntax

  Žiga Avsec, et al., bioRxiv - Genomics 2020

  Quote: ... ɑ-Klf4 (R&D Systems, AF3158), ɑ-Klf4 (Abcam ...
• Astrocyte- and neuron-derived extracellular vesicles from Alzheimer’s disease patients effect complement-mediated neurotoxicity

  Carlos J Nogueras-Ortiz, et al., bioRxiv - Neuroscience 2020

  Quote: ... Primary neurons and EVs were pre-incubated with active recombinant human CD59 (cat. no. 1987-CD, R&D Systems) at a final concentration of 1 μM (stock at 10 μM ...
• Astrocyte- and neuron-derived extracellular vesicles from Alzheimer’s disease patients effect complement-mediated neurotoxicity

  Carlos J Nogueras-Ortiz, et al., bioRxiv - Neuroscience 2020

  Quote: ... Licor Biosciences) targeting apolipoprotein A1 (0.4 μg/mL; RRID: AB_2242717, cat. no. AF3664, R&D Systems, Minneapolis, MN) as an indicator of lipoproteins ...
• Astrocyte- and neuron-derived extracellular vesicles from Alzheimer’s disease patients effect complement-mediated neurotoxicity

  Carlos J Nogueras-Ortiz, et al., bioRxiv - Neuroscience 2020

  Quote: ... DAF (cat. no. 2009-CD) and CD59 (cat. no. 1987-CD) were acquired from R&D Systems, whereas Factor I (cat ...
• Granzyme A inhibition reduces inflammation and increases survival during abdominal sepsis

  Marcela Garzón-Tituaña, et al., bioRxiv - Immunology 2020

  Quote: ... Human serum GzmA levels from healthy donors and patients with confirmed abdominal sepsis at diagnose and during the first 72h was monitored by a commercial ELISA kit (Human Granzyme A DuoSet ELISA, R&D Systems) following the manufacturer recommendations.
• Cadherin-11 is required for neural crest determination and survival

  Subrajaa Manohar, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Rabbit α-Active Caspase-3 (R&D Systems, #AF835), Mouse α-PAX7 (DSHB) ...
• Gain-of-function mutation in Gli3 causes ventricular septal defects

  Antonia Wiegering, et al., bioRxiv - Developmental Biology 2020

  Quote: Western blot studies were performed as described earlier using an anti-GLI2 (#AF3635; R&D Systems) antibody (Wang et al. ...
• Gain-of-function mutation in Gli3 causes ventricular septal defects

  Antonia Wiegering, et al., bioRxiv - Developmental Biology 2020

  Quote: ... GLI2 (#AF3635; R&D Systems), KI67 (#sc-7846 ...
• MicroRNA-dependent inhibition of PFN2 orchestrates ERK activation and pluripotent state transitions by regulating endocytosis

  Carolyn Sangokoya, Robert Blelloch, bioRxiv - Developmental Biology 2020

  Quote: ... cells were treated with 100ng/mL human recombinant FGF4 (R&D Systems, 235-F4) in the presence of 10 μg/mL heparin sulfate and 3 μM BI-D1870 RSK inhibitor at 37°C ...
• Differential regulation of lineage commitment in human and mouse primed pluripotent stem cells by NuRD

  Ramy Ragheb, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 10 ng/ml BMP4 (R&D Systems), 10 µM LY294002 (Promega ...
• A Chemokine-Fusion Vaccine Targeting Immature Dendritic Cells Elicits Elevated Antibody Responses to Malaria Sporozoites in Infant Macaques

  Kun Luo, et al., bioRxiv - Immunology 2020

  Quote: ... recombinant purified mouse MIP3α (R&D Systems, Minneapolis, MN) was used (2 μg/well ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... supplemented with BMP4 (3 ng/ml, R&D Systems) and Thiazovivin (2 μM ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... basic FGF (bFGF, 2.5 ng/ml, R&D Systems), and BMP4 (0.5 ng/ml ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and vascular endothelial growth factor (VEGF, 10 ng/ ml, R&D Systems). On day 5 EBs were dissociated and analyzed for CXCR4+ cKIT+ cells ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and BMP4 (0.5 ng/ml, R&D Systems). At day 4 ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... At day 1 the medium was changed to base media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
• Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

  Young Sun Hwang, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 50ng/mL EGF (R&D Systems, 236-EG), 1,000 U/ml LIF (Millipore ...
• Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

  Young Sun Hwang, et al., bioRxiv - Developmental Biology 2020

  Quote: ... at 3500 cells per well in GK15 medium supplemented with 200 ng/ml BMP4 (R&D Systems, 314-BP-010), 100 ng/ml SCF (R&D Systems ...
• Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

  Young Sun Hwang, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 100 ng/ml SCF (R&D Systems, 255-SC-010), 50ng/mL EGF (R&D Systems ...
• Activation of mitochondria is an acute Akt-dependent response during osteogenic differentiation

  C. Owen Smith, Roman Eliseev, bioRxiv - Developmental Biology 2020

  Quote: ... 0.2 ng/mL TGFβ (R&D systems 240-B), or 1 ng/mL PTH aa1-34 (R&D systems 3011/1 ...
• Activation of mitochondria is an acute Akt-dependent response during osteogenic differentiation

  C. Owen Smith, Roman Eliseev, bioRxiv - Developmental Biology 2020

  Quote: ... 25 ng/mL Wnt3a (R&D systems 5036-WN-010), 5 ng/mL IGF1 (Sigma 13769-50UG) ...
• DOT1L methyltransferase regulates the calcium influx in erythroid progenitor cells in response to erythropoietin

  Yi Feng, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Mouse EPO (R&D systems) was added to a final concentration of 10 units/ml ...
• Derivation of Isogenic Mesodermal and Ectomesodermal Chondrocytes from Human Induced Pluripotent Stem Cells for Articular Cartilage Regeneration

  Ming-Song Lee, et al., bioRxiv - Developmental Biology 2020

  Quote: ... hiPSCs were incubated with the primary antibody goat anti-human OCT3/4 (R&D Systems, Minneapolis, MN, USA), mouse anti-human SOX2 (R&D Systems) ...
• Derivation of Isogenic Mesodermal and Ectomesodermal Chondrocytes from Human Induced Pluripotent Stem Cells for Articular Cartilage Regeneration

  Ming-Song Lee, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-human PODPCALYXIN (R&D Systems), mouse anti-human SSEA4 (R&D Systems) ...
• Derivation of Isogenic Mesodermal and Ectomesodermal Chondrocytes from Human Induced Pluripotent Stem Cells for Articular Cartilage Regeneration

  Ming-Song Lee, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-human SSEA4 (R&D Systems), or mouse anti-human CD9 (R&D Systems ...
• Derivation of Isogenic Mesodermal and Ectomesodermal Chondrocytes from Human Induced Pluripotent Stem Cells for Articular Cartilage Regeneration

  Ming-Song Lee, et al., bioRxiv - Developmental Biology 2020

  Quote: ... or mouse anti-human CD9 (R&D Systems) for 30 min at 4°C ...
• Derivation of Isogenic Mesodermal and Ectomesodermal Chondrocytes from Human Induced Pluripotent Stem Cells for Articular Cartilage Regeneration

  Ming-Song Lee, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-human SOX2 (R&D Systems), goat anti-human NANOG (R&D Systems) ...
• Derivation of Isogenic Mesodermal and Ectomesodermal Chondrocytes from Human Induced Pluripotent Stem Cells for Articular Cartilage Regeneration

  Ming-Song Lee, et al., bioRxiv - Developmental Biology 2020

  Quote: ... goat anti-human NANOG (R&D Systems), mouse anti-human PODPCALYXIN (R&D Systems) ...
• The BMP antagonist Gremlin1 contributes to the development of cortical excitatory neurons, motor balance and fear responses

  Mari Ichinose, et al., bioRxiv - Developmental Biology 2020

  Quote: ... the membranes were incubated overnight at 4°C with anti-Gremlin1 antibody (R&D systems, AF956, 1:1000) or anti-βActin (Santa Cruz ...
• Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells

  Diana Gumber, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 40ng/mL SCF (R&D Systems), 20ng/mL VEGF (R&D System) ...
• Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells

  Diana Gumber, et al., bioRxiv - Developmental Biology 2020

  Quote: ... with 40ng/mL BMP4 (R&D Systems), 40ng/mL SCF (R&D Systems) ...
• Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells

  Diana Gumber, et al., bioRxiv - Developmental Biology 2020

  Quote: ... 20ng/mL VEGF (R&D System), 10ng/mL FGF-2 (StemCell Technology) ...
• Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells

  Diana Gumber, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Activin A (100 ng/ml, R&D Systems) and a treatment for the first day ...
• Ubc9 deletion in adipocytes causes lipoatrophy in mice

  Aaron R. Cox, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and FGF21 (#MF2100; R&D Systems). Hepatic triglyceride content was quantified by Thermo Scientific Triglycerides Reagent (#TR22421 ...
• Interspecies Chimeric Conditions Affect the Developmental Rate of Human Pluripotent Stem Cells

  Jared Brown, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and 5ng/mL bFGF (R&D Systems, USA). Cells were passaged by adding TrypLE (Thermo Fisher Scientific ...
• Hnf4a is required for the development of Cdh6-expressing progenitors into proximal tubules in the mouse kidney

  Sierra S. Marable, Eunah Chung, Joo-Seop Park, bioRxiv - Developmental Biology 2020

  Quote: ... Cdh6 (1:100, R&D Systems MAB2715-SP), and Cdh6 (1:200 ...
• Context-dependent functional compensation between Ythdf m6A readers

  Lior Lasman, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Esrrb (R&D systems, PP-H6705-00), Oct4 (Santa Cruz ...
• The extracellular matrix protein agrin is essential for epicardial epithelial-to-mesenchymal transition during heart development

  Xin Sun, et al., bioRxiv - Developmental Biology 2020

  Quote: ... while WNT3A (R&D Systems) was used at a concentration of 50ng/ml ...
• The extracellular matrix protein agrin is essential for epicardial epithelial-to-mesenchymal transition during heart development

  Xin Sun, et al., bioRxiv - Developmental Biology 2020

  Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
• Activation of mitochondria is an acute Akt-dependent response during osteogenic differentiation

  C. Owen Smith, Roman Eliseev, bioRxiv - Developmental Biology 2020

  Quote: ... or 1 ng/mL PTH aa1-34 (R&D systems 3011/1) to αMEM media induced osteogenesis ...
• Prrx1b directs pro-regenerative fibroblasts during zebrafish heart regeneration

  Dennis E.M. de Bakker, et al., bioRxiv - Developmental Biology 2020

  Quote: ... An NRG1-β1 ELISA assay was performed according to the protocol of the manufacturer (Human NRG1-β1 DuoSet ELISA, R&D systems). Absolute NRG1-β1 concentration was calculated based on the standard curve.
• Zfp36l1 and Zfp36l2 balances proliferation and differentiation in the developing retina

  Fuguo Wu, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-Nr2e3 (R&D systems PP-H7223,1:100), goat anti-Nrl (R&D systems AF2945 ...