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Citations for R&D Systems :
751 - 800 of 1736 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the vessels were incubated with either PBS or different concentrations of recombinant mouse CXCL9 protein (R&D systems, catalog number 492-MM) for 3-4 hrs ...
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bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
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bioRxiv - Cancer Biology 2020Quote: ... The overexpression and loss of ICAM-1 protein were validated by flow cytometry with an anti-ICAM1 antibody (BBA20, R&D Systems). Besides ...
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bioRxiv - Cell Biology 2021Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: Relative phosphorylation levels of 43 kinases and 2 related proteins were assessed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Each anti-VEGF-A injection consisted of 200 μL of 32.5 pM concentration of antibody against mouseVEGF-A164 protein (YU1418071, R&D Systems, Inc.) in saline ...
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bioRxiv - Biochemistry 2020Quote: All proteins described herein for in vitro analyses were expressed and purified as recombinant proteins with the exception of E1 activating enzyme UBE1 and heavy isotope labeled ubiquitin (both purchased from R&D Systems). Recombinant ubiquitin ...
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bioRxiv - Systems Biology 2019Quote: ... five µg of glycopeptide in 50 mM Tris-HCl pH 7.4 was mixed with one µg recombinant human C1GalT1/C1GalT1C1 protein (R&D Systems, NM) in the presence of either 0.5 mM UDP-Gal (Sigma-Aldrich ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... respectively) and recombinant proteins used for quantification (8614-MT, 344-ER, 1129-ER, and 348-RB respectively) were from R&D systems. Streptavidin Phycoerythrin (SAPE ...
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bioRxiv - Immunology 2020Quote: Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
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bioRxiv - Cell Biology 2019Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Neuroscience 2019Quote: ... Plasma interleukin (IL)-6 and lipopolysaccharide-binding protein (LBP) levels were measured by commercially available enzyme-linked immunosorbent assay (R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2021Quote: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25-1.25 µg/ml recombinant human rSCUBE3 protein (Cat # 7730-SC, R&D Systems, Abingdon, UK) in the presence of RANKL and M-CSF ...
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bioRxiv - Immunology 2020Quote: ... cells were preincubated for 5 min at room temperature with 1 µg of the unlabeled PS-binding proteins MFG-E8 (R&D systems) and AnV (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 protein was detected by Hamster IL-6 Sandwich ELISA Kit (AssayGenie) or Mouse IL-6 Duoset Sandwich ELISA kit (R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206 ...
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bioRxiv - Neuroscience 2022Quote: ... 506-RL-010/CF) and carrier free Recombinant Mouse TNF-alpha (aa 80-235) Protein (410-MT-025/CF) purchased from R&D Systems.
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bioRxiv - Cell Biology 2023Quote: ... The samples were quantified by BCA assay and further equal protein was assayed by enzyme-linked immunosorbent assay (ELISA) for TNF-a using the manufacturer’s protocol (R&D systems, USA).
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Neuroscience 2024Quote: ... Relative expression levels of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... after which the organoids were cultured for 13 days in E6 supplemented with 50 ng/mL bone morphogenetic protein-7 (BMP7, R&D systems) and 10 ng/mL human epidermal growth factor (hEGF ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
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bioRxiv - Molecular Biology 2022Quote: ... Lot #: 413-ML-005) and gremlin 1 (Cat #: 956-GR-050, Lot #: ESD4616121) recombinant proteins were all purchased from R&D Systems. Gusb ...
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bioRxiv - Immunology 2023Quote: ... Levels of IL1β protein were determined in culture supernatant using the IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
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bioRxiv - Cancer Biology 2023Quote: We assessed binding to a library of 300 glycan structures for Fc fusion proteins sourced from a commercial vendor (R&D Systems) composed of a fusion of Siglec-3 ...
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bioRxiv - Neuroscience 2023Quote: ... Relative expression level of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: ... Unpurified transient transfection supernatant samples (50 µl) were pre-incubated with biotinylated SARS-CoV-2 spike protein (R&D Systems cat# BT10549) (50 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...
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bioRxiv - Cell Biology 2023Quote: ... RFP labeled eEPCs were plated with monolayer and 10ng/ml of recombinant human VEGF protein (R&D System, Cat# 293-VE-010) was treated to enhance tube formation ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
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bioRxiv - Immunology 2023Quote: ... KIR2DL3*001 (#2014-KR-050) and KIR2DS4*001 (#1847-KR-050) KIR-Ig (KIR-Fc) fusion proteins were purchased from R&D systems. KIR2DS1*001-Fc was custom produced by Sino Biological ...
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bioRxiv - Immunology 2023Quote: ... Levels of BAFF protein were determined in culture supernatant or bone marrow plasma using the human BAFF/BLyS/TNFSF13B DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of CXCL1 or CXCL2 protein in the supernatant was determined using a mouse CXCL1 or CXCL2 specific ELISA kit (R&D system). All experiments were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... co-cultures were maintained for 24 hours in the control condition or in the presence of the chimera proteins Fc-LGR4 (8077-GP, R&D Systems), Fc-LGR5 (8078-GP ...
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bioRxiv - Immunology 2024Quote: ... For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050, R&D Systems) at 1 µg/ml.
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... and acute phase proteins (total 36) present in the supernatants were detected with using Proteome Profiler Human Cytokines Array Kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Pathology 2023Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in B cell Excellerate medium with the CEllXVivo B cell expansion kit (R&D systems, UK). CD8+ T-cells (Cambridge Bioscience ...
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bioRxiv - Bioengineering 2024Quote: GFP positive choroidal endothelial cells (ICEC2-TS) were cultured in complete endothelial cell growth media (R&D systems) containing 1% primocin (InvivoGen ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...