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Citations for R&D Systems :
651 - 700 of 1736 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown to 70% confluent in 24-well plates prior to treatment with N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89; R&D Systems) or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Immunology 2019Quote: ... Immature dendritic cells were prepared by treatment of monocytes with 500 U/ml human IL-4 and 800 U/ml human GM-CSF (R&D Systems) in CellGenix media for 6 days ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... the media were refreshed and supplemented with 5 ng/mL IL-7 or 5 ng/mL IL-7 and 4 ng/ml IL-2 (R&D Systems), respectively ...
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bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Neuroscience 2024Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM, R&D systems). To monitor and ensure the genetic stability of the cells ...
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bioRxiv - Immunology 2024Quote: ... Adherent cell were then cultured in RPMI supplemented with 10% heat-inactivated human serum and L-Glutamine and gentamycin as above (RPMI10%HuS) and 30 ng/ml GM-CSF (Immunex) and 10 ng/ml IL-4 (R&D Systems) for 5 days50 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Cancer Biology 2024Quote: A pool of plasma from 2 to 4 mice was loaded on the membrane of the Proteome Profiler Mouse XL cytokine Array (ARY028, R&D systems) following manufacturer’s recommendations ...
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bioRxiv - Physiology 2024Quote: ... the platelets were incubated at 4°C over night with antibodies against CXCR4 (MAB172, Clone # 44716, R&D Systems, Minneapolis, Minnesota, USA), ACKR3 (ab72100 ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations was determined by Thermo Fisher BCA assay kit and then 50μg of protein was loaded into the pre-coated VEGFA ELISA kit (R&D systems). Assay per manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
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bioRxiv - Developmental Biology 2019Quote: ... with mouse and human soluble FLT1-FC chimeric protein (471-F1-100 and 321-FL-050/CF, R&D Systems) immobilized to about 1,000 RU was used as ligand for the analyte binding of anti-FLT1 MAbs ...
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bioRxiv - Developmental Biology 2019Quote: ... anti-FLT1 antibodies were incubated with mouse FLT1-FC chimeric protein with His-tag (471-F1-100, R&D Systems). After incubation the antibody-FLT1-FC complex was added to the VEGFA-coated plates ...
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bioRxiv - Pathology 2021Quote: ... Gdf8 and Smad2/3 proteins were quantified by ELISA methods (GDF8/Myostatin Quantikine ELISA kit, Cat # DGDF80, R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2024Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... 200μg of total mouse protein samples were analyzed respectively with a Mouse XL Cytokine Array Kit (R&D Systems; ARY028) per manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of angiogenic proteins in CM was measured using a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D systems) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Immunology 2024Quote: YM1 and CCL17 protein levels were measured in PCLS (4mm diameter) supernatant using commercially available ELISA kits (R&D Systems), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO, Santa Cruz; and Gli1, R&D Systems) diluted with 1% BSA/0.3% Triton-X/PBS solution ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Physiology 2020Quote: ... non-esterified fatty acid (NEFA) serum levels were measured by colorimetric assay (Randox, UK) and dipeptidyl peptidase 4 (DPP4; R&D Systems, UK) levels were measured by ELISA.
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific, 114.13D; R&D Systems, MAGM204) were intravenously injected to an actin-DsRed mouse ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...