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7001 - 7050 of 10000+ citations since 2019

• The cis-regulatory effects of modern human-specific variants

  Carly V. Weiss, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... 200 ng/mL Recombinant mouse Noggin (R&D systems), and 10 μM SB431542 (EMD Millipore) ...
• Cooperative virulence can emerge via horizontal gene transfer but is stabilized by transmission

  Erik Bakkeren, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... Lipocalin-2 ELISA (R&D Systems kit ...
• The cis-regulatory effects of modern human-specific variants

  Carly V. Weiss, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... with B27 (Thermo Fisher Scientific)] supplemented with 20 ng/mL bFGF (R&D systems) and 20 ng/mL EGF (Millipore sigma)] on Matrigel-coated dish ...
• A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite Adipogens

  Stephanie Kim, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... diluted 1:200 and analyzed for adiponectin by ELISA following the manufacturer’s instructions (Mouse Adiponectin/Acrp30 Quantikine ELISA Kit, R&D Systems, MRP300). Absorbance (450 nM ...
• Generation of a novel Nkx6-1 Venus fusion reporter mouse line

  Ingo Burtscher, et al., bioRxiv - Developmental Biology 2021

  Quote: ... anti-Nkx6-1 (Goat 1:200; R&D Systems AF5857). Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT ...
• Generation of a novel Nkx6-1 Venus fusion reporter mouse line

  Ingo Burtscher, et al., bioRxiv - Developmental Biology 2021

  Quote: ... anti-Nkx6-1 (Goat 1:300; R&D Systems AF5857), anti-Flag (Mouse HRP ...
• Fibrin Polymer on the Surface of Biomaterial Implants Drives the Foreign Body Reaction

  Arnat Balabiyev, et al., bioRxiv - Immunology 2021

  Quote: ... The mouse anti-α -SMA mAb (catalog number MAB1420-SP) was from R&D systems. The rat anti-CD68 mAb (catalog number 14-0681-82 ...
• Maf is a regulator of differentiation for gut immune epithelial cell Microfold cell (M cell)

  Joel Johnson George, et al., bioRxiv - Developmental Biology 2021

  Quote: ... recombinant mouse R-spondin1 (1 μg/mL; R&D Systems). Media were changed every 2 days ...
• Nephronectin-Integrin α8 signaling is required for proper migration of periocular neural crest cells during chick corneal development

  Justin Ma, Lian Bi, James Spurlin, Peter Lwigale, bioRxiv - Developmental Biology 2021

  Quote: ... followed by recombinant mouse or human Npnt (R&D Systems) at 1.5 μg/cm2 for 2 hours at 37 °C ...
• Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis

  Xiaodong Li, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 1x N2-plus supplement (R&D Systems, Cat#AR003), and 1x penicillin/streptomycin (Thermo Fisher Scientific ...
• Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis

  Xiaodong Li, et al., bioRxiv - Developmental Biology 2021

  Quote: ... coli-derived human N-terminal modified (C24II) fragment of Sonic Hedgehog (Shh-N; R&D Systems, Cat#1845-SH/CF) was reconstituted in PBS at 10µM and stored at -80°C ...
• Molluscan dorsal-ventral patterning relying on bmp2/4 and chordin provides insights into spiralian development and bilaterian evolution

  Sujian Tan, Pin Huan, Baozhong Liu, bioRxiv - Developmental Biology 2021

  Quote: rhBMP4 (R&D Systems, USA; Cat. No. 314-BP) was resuspended in the suspending solution (0.2% BSA containing 4 mM HCl ...
• Biphasic roles of hedgehog signaling in the production and self-renewal of outer radial glia in the ferret cerebral cortex

  Shirui Hou, et al., bioRxiv - Developmental Biology 2021

  Quote: ... sheep anti-PAX6 (AF8150: R&D Systems, 5 µg/mL); rat anti-TBR2 (clone ...
• Single-cell analysis of early chick hypothalamic development reveals that hypothalamic cells are induced from prethalamic-like progenitors

  Dong Won Kim, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Explants were either treated with recombinant FST (7.5 μg/ml, Cat no. 769-FS-025, R&D Systems) or anti-FST antibody (20 μ/ml ...
• Generation of human blastocyst-like structures from pluripotent stem cells

  Yong Fan, et al., bioRxiv - Developmental Biology 2021

  Quote: ... supplemented with 25ng/ml BMP4 (R&D SYSTEMS, 314-BP-010), 2µM Y-27632 (Selleck ...
• Chromatin accessibility and microRNA expression in nephron progenitor cells during kidney development

  Andrew Clugston, et al., bioRxiv - Developmental Biology 2021

  Quote: ... biotinylated to antibodies for Integrin alpha 8 (Itgα8, R&D Systems AF4076) using the DSB-X Biotin Protein Labeling Kit (ThermoFisher D-20655) ...
• Generation of human blastocyst-like structures from pluripotent stem cells

  Yong Fan, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Human N2B27-LCDM medium consisted of N2B27 basal medium supplemented with 10 ng/mL human LIF (R&D Systems, 7734), 3µM CHIR99021 (Tocris ...
• General and cell-type-specific aspects of the motor neuron maturation transcriptional program

  Tulsi Patel, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Spp1 (Mouse, R&D Systems AF808, 1:50). Primary antibody incubations were done overnight at 4C except for Chat ...
• Hierarchical reactivation of transcription during mitosis-to-G1 transition by Brn2 and Ascl1 in neural stem cells

  Mário A. F. Soares, et al., bioRxiv - Developmental Biology 2021

  Quote: ... or snapshot acquisition after cell synchronization in metaphase using 60μM proTAME (R&D systems, #I-440-01M) and 200μM Apcin (R&D systems ...
• Hierarchical reactivation of transcription during mitosis-to-G1 transition by Brn2 and Ascl1 in neural stem cells

  Mário A. F. Soares, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and 200μM Apcin (R&D systems, #I-444-05M) for 2-6 hours ...
• An activating mutation in Pdgfrb causes skeletal stem cell defects with osteopenia and overgrowth in mice

  Hae Ryong Kwon, et al., bioRxiv - Developmental Biology 2021

  Quote: ... cells were starved in medium containing 0.1% FBS (mesenchymal stem cell-qualified) for 24 hours and treated with 10 ng/ml PDGF-BB (R&D systems) for 10-15 min ...
• The inflammasome-activated cytokine IL-1β is targeted for ubiquitylation and proteasomal degradation to limit its inflammatory potential

  Swarna L. Vijayaraj, et al., bioRxiv - Immunology 2021

  Quote: ... pro and mature IL-1β (R&D Systems; AF-401-NA), pro- and cleaved Caspase-1 (Adipogen ...
• Ischemic tolerance and cardiac repair in the African spiny mouse

  Tim Koopmans, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Angiogenic proteins were measured using a Mouse Angiogenesis Array Kit (R&D Systems, #ARY015) according to the manufacturer’s instructions ...
• The inflammasome-activated cytokine IL-1β is targeted for ubiquitylation and proteasomal degradation to limit its inflammatory potential

  Swarna L. Vijayaraj, et al., bioRxiv - Immunology 2021

  Quote: ... ZVAD-fmk (20 μM, Z-VAD-FMK, R&D Systems), 911 (1 μM ...
• The inflammasome-activated cytokine IL-1β is targeted for ubiquitylation and proteasomal degradation to limit its inflammatory potential

  Swarna L. Vijayaraj, et al., bioRxiv - Immunology 2021

  Quote: ... Macrophages and neutrophils were primed where indicated with recombinant murine IFN-g (50 ng/ml, R&D Systems) for 1 hour (for neutrophil cultures ...
• Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2

  E.A. Rosado-Olivieri, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti-Active Caspase-3 (R&D Systems; AF835; 1:250); mouse anti-Mucin 5AC (Abcam ...
• Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2

  E.A. Rosado-Olivieri, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-SOX2 (R&D Systems; AF2018; 1:250), rabbit anti-NKX2.1 (Abcam ...
• Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2

  E.A. Rosado-Olivieri, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and goat anti-SOX17 (R&D Systems; AF1924; 1:200). To detect infected cells for HCoV-229E ...
• Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2

  E.A. Rosado-Olivieri, et al., bioRxiv - Developmental Biology 2021

  Quote: ... micropattern cultures were fed in lung induction medium (LIM) which contains cSFDM supplemented with 50 ng*mL−1 KGF (or indicated in each experiment; R&D systems), 10 ng*mL−1 BMP4 (R&D systems) ...
• Self-organized stem cell-derived human lung buds with proximo-distal patterning and novel targets of SARS-CoV-2

  E.A. Rosado-Olivieri, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 10 ng*mL−1 BMP4 (R&D systems), 100 nM retinoic acid (“RA” ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and seeded at 6×105/cm2 or 3×105/cm2 (corresponding to high or low cell density) on 24-well transwell plates in MEF-CM supplemented with 20 ng/ml bFGF (R&D Systems), 10 μM ROCK inhibitor (Y-27632 ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... or BMP4 (R&D Systems) for 1 hour the following day ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 8×105 cells were seeded on each coverslip in a defined volume of MEF-CM supplemented with 20ng/ml bFGF (R&D Systems), 10 μM ROCK inhibitor (Y-27632 ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and/or 100 ng/ml ACTIVIN (R&D Systems). Fixed samples were analyzed by immunofluorescence 1 ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Cells were stimulated with 10 ng/ml ACTIVIN (R&D Systems) or BMP4 (R&D Systems ...
• Huntingtin CAG expansion impairs germ layer patterning in synthetic human gastruloids through polarity defects

  Szilvia Galgoczi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 100 ng/ml WNT3a (R&D Systems), 6 μM CHIR99021 (EMD Millipore ...
• Patient brain organoids identify a link between the 16p11.2 copy number variant and the RBFOX1 gene

  Milos Kostic, et al., bioRxiv - Neuroscience 2021

  Quote: ... 20 ng/ml EGF (R&D systems, 236-EG-01M) and 20 ng/ml FGF (R&D systems ...
• Patient brain organoids identify a link between the 16p11.2 copy number variant and the RBFOX1 gene

  Milos Kostic, et al., bioRxiv - Neuroscience 2021

  Quote: ... and 20 ng/ml FGF (R&D systems, 233-FB-025) both reconstituted in 0.1% Bovine Serum Albumin ...
• Convergent Cerebrospinal Fluid Proteomes and Metabolic Ontologies in Humans and Animal Models of Rett Syndrome

  Stephanie A. Zlatic, et al., bioRxiv - Neuroscience 2021

  Quote: ... and 40ng/ml beta-FGF (R&D Systems 4114-TC-01M). After 24 hours ...
• Convergent Cerebrospinal Fluid Proteomes and Metabolic Ontologies in Humans and Animal Models of Rett Syndrome

  Stephanie A. Zlatic, et al., bioRxiv - Neuroscience 2021

  Quote: ... and 2 ng/ml GDNF (R&D Systems 212-GD-050). Cells conditioned the media for 3 days at 5% CO2 in a 37°C incubator ...
• Nuclear Factor I in neurons, glia and during the formation of Müller glia-derived progenitor cells in avian, porcine and primate retinas

  Heithem M. El-Hodiri, et al., bioRxiv - Neuroscience 2021

  Quote: ... (11) goat anti-Otx2 was used at 1:1000 (AF1979; R&D Systems). This antibody was raised to recombinant human Otx2 (Met1-Leu289) ...
• The neural cell adhesion molecule NrCAM regulates development of hypothalamic tanycytes

  Alex Moore, et al., bioRxiv - Neuroscience 2021

  Quote: ... supplemented with 3 nM Shh (1845-SH; R&D Systems), as used in Ohyama et al (2005 ...
• Nuclear bundle/cable containing actin during yeast meiosis

  Tomoko Takagi, Masako Osumi, Akira Shinohara, bioRxiv - Cell Biology 2021

  Quote: ... cells were treated with 30 μM Latrunculin B (R&D Systems) dissolved in 0.1% DMSO at 30°C for 1 hour ...
• Astrocytic C-X-C motif chemokine ligand-1 mediates β-amyloid-induced synaptotoxicity

  Beatriz Gomez Perez-Nievas, et al., bioRxiv - Neuroscience 2021

  Quote: The cytoplasmic fraction of human postmortem brain homogenates and astrocyte culture media were used to determine cytokine content using Mouse or Human Proteome Profiler arrays (Mouse Cytokine Array Panel A and Human Cytokine Array, R&D Systems), according to the manufacturers’ instructions ...
• Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative disease

  Robert N. Plasschaert, et al., bioRxiv - Neuroscience 2021

  Quote: ... Sections were first incubated with an antigen retrieval solution (Antigen Retrieval Reagent-Basic, R&D Systems) at 80°C for 30 minutes followed by blocking (2% non-fat dry milk ...
• Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative disease

  Robert N. Plasschaert, et al., bioRxiv - Neuroscience 2021

  Quote: ... Sections were then incubated with primary antibodies for progranulin (AF2420 R&D Systems) and NeuN (MAB377 MilliporeSigma ...
• Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative disease

  Robert N. Plasschaert, et al., bioRxiv - Neuroscience 2021

  Quote: ... Progranulin protein levels were measured using a human-specific ELISA (Quantikine and DuoSet; R&D Systems) according to the manufacturer’s instructions ...
• Microglia drive pockets of neuroinflammation in middle age

  Eric N. Moca, et al., bioRxiv - Neuroscience 2021

  Quote: ... goat anti-DTR (R&D Systems AF259), rat anti-CD68 (1:200 ...
• Neurodevelopmental processes in the prefrontal cortex derailed by chronic HIV-1 viral protein exposure

  Kristen A. McLaurin, et al., bioRxiv - Neuroscience 2021

  Quote: ... A commercial ELISA kit was utilized to evaluate levels of IL-1β (R&D Systems, Inc., Minneapolis, MN), the cytokine with the highest levels of expression in RT-PCR ...