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6901 - 6950 of 10000+ citations since 2019

• A Mouse Model of Brittle Cornea Syndrome caused by mutation in Zfp469

  Chloe M. Stanton, et al., bioRxiv - Genetics 2021

  Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
• Intronic regulation of SARS-CoV-2 receptor (ACE2) expression mediated by immune signaling and oxidative stress pathways

  Daniel Richard, et al., bioRxiv - Genetics 2021

  Quote: ... and 2 μg/ml of mouse anti-ACE2 (R&D Systems;; Catalogue: MAB933;; RRID: AB_2223153). Secondary antibodies used were ...
• Transcriptional Reprogramming of Skeletal Muscle Stem Cells by the Niche Environment

  Felicia Lazure, et al., bioRxiv - Genetics 2021

  Quote: ... Antibodies for anti-mouse ITGA7-AF647 (R&D systems, FAB3518R), anti-mouse CD31-PE (Invitrogen 12-0311-81) ...
• GP2-enriched pancreatic progenitors give rise to functional beta cells in vivo and eliminate the risk of teratoma formation

  Yasaman Aghazadeh, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and resuspended in unconjugated PDX1 (1/100, R&D Systems) and NKX6.1 (1/1500 ...
• GP2-enriched pancreatic progenitors give rise to functional beta cells in vivo and eliminate the risk of teratoma formation

  Yasaman Aghazadeh, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and 500 ng/ml Noggin (R&D Systems) to promote neuroectoderm differentiation ...
• Tyr is Responsible for the Cctq1a QTL and Links Developmental Environment to Central Corneal Thickness Determination

  Kacie J. Meyer, et al., bioRxiv - Genetics 2021

  Quote: ... Four Ctsc mutations were propagated in separate intercross mouse lines and were validated as null mutations by Western Blot using an antibody against CTSC (Catalog #AF1034; R&D Systems, Inc. ...
• Interpreting ciliopathy-associated missense variants of uncertain significance (VUS) in an animal model

  Karen I. Lange, et al., bioRxiv - Genetics 2021

  Quote: ... goat anti-ROR2 (1:1000, AF2064; R&D Systems Inc., Minneapolis, MN, USA). Appropriate HRP-conjugated secondary antibodies (Dako UK Ltd. ...
• Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

  Antigoni Gogolou, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
• Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

  Antigoni Gogolou, et al., bioRxiv - Developmental Biology 2021

  Quote: ... FGF2 (20 ng/ml, R&D Systems) and Rho-Associated Coil Kinase (ROCK ...
• The developing mouse coronal suture at single-cell resolution

  D’Juan T. Farmer, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and Cd200 (1:200; AF2724, R&D systems) or Tcf7 (1:200 ...
• Single cell trajectory analysis of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

  Chaido Ori, et al., bioRxiv - Developmental Biology 2021

  Quote: ... supplemented with 10 μM Y-27632 (R&D Systems) for the first 24 hours ...
• Single cell trajectory analysis of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

  Chaido Ori, et al., bioRxiv - Developmental Biology 2021

  Quote: ... the medium was switched to FE-BM1 or BM2 containing 20 ng/ml recombinant human BMP4 (rhBMP4, R&D Systems), 50 nM retinoic acid (RA ...
• Single cell trajectory analysis of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

  Chaido Ori, et al., bioRxiv - Developmental Biology 2021

  Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
• The developing mouse coronal suture at single-cell resolution

  D’Juan T. Farmer, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and Dmp1 (1:400; AF4386, R&D systems) primary antibodies ...
• Single cell trajectory analysis of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

  Chaido Ori, et al., bioRxiv - Developmental Biology 2021

  Quote: ... and 10 μM Y-27632 (all from R&D Systems), in Definitive Endoderm Basal Media (DE-BM ...
• Single cell trajectory analysis of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

  Chaido Ori, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 3 μM CHIR99021 and 20 ng/ml rhFGF10 (R&D Systems). For the inhibition of Notch and TGF-β pathways ...
• Single-cell transcriptomic analysis unveils spinal motor neuron subtype diversity underpinning the water-to-land transition in vertebrates

  Ee Shan Liau, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-Foxp1(1:100 R&D systems Cat# AF4534, RRID:AB_2107102); rabbit anti-Hoxc8 (1:5000 Sigma-Aldrich Cat# HPA028911 ...
• Single-cell transcriptomic analysis unveils spinal motor neuron subtype diversity underpinning the water-to-land transition in vertebrates

  Ee Shan Liau, et al., bioRxiv - Developmental Biology 2021

  Quote: ... mouse anti-COUP-TF2/NR2F2 (1:200 R&D systems Cat# PP-H7147-00, RRID:AB_2155627); rabbit anti-Evi1/Mecom (1:500 Cell Signaling Technology Cat# 2593 ...
• Extraembryonic endoderm cells induce neuroepithelial tissue in gastruloids

  Noémie M. L. P. Bérenger-Currias, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-GATA6 (1:200, AF1700, R&D Systems), rabbit anti-Laminin (1:200 ...
• Extraembryonic endoderm cells induce neuroepithelial tissue in gastruloids

  Noémie M. L. P. Bérenger-Currias, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-T (1:100, AF2085, R&D systems), mouse anti-DAB2 (1:100 ...
• Single-cell analysis of early chick hypothalamic development reveals that hypothalamic cells are induced from prethalamic-like progenitors

  Dong Won Kim, et al., bioRxiv - Developmental Biology 2021

  Quote: ... or anti-FST antibody (20 μ/ml, AF669, R&D Systems) for 42 hr ...
• Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents

  Raghu Ram Katreddi, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Goat anti-Dll4 (*with antigen retrieval 1:50 and without antigen retrieval 1:25, AF1389, R&D Systems), *Rabbit anti-NICD activated (1:75 ...
• Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad

  Chloé Mayère, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-SOX9 (1:1000, AF3075, R&D Systems), rat anti-LAMB1 (1:500 ...
• Spatial and temporal expression of PORCN is highly dynamic in the developing mouse cochlea

  Brianna L. Oliver, et al., bioRxiv - Developmental Biology 2021

  Quote: ... goat α-SOX2 (R&D Systems, Minneapolis, Minnesota, USA, AF2018), rabbit α-GSK3β (Cell Signaling ...
• A transcriptome atlas of the mouse iris at single cell resolution defines cell types and the genomic response to pupil dilation

  Jie Wang, Amir Rattner, Jeremy Nathans, bioRxiv - Developmental Biology 2021

  Quote: ... goat anti-ZIC1 (R&D system AF4978-SP); rabbit anti-NeuN (Cell Signaling Technologies 24307S) ...
• Epigenetic modulation in the pathogenesis and treatment of inherited aortic aneurysm conditions

  Benjamin E. Kang, et al., bioRxiv - Genetics 2021

  Quote: ... Stimulation was performed using 10 ng/ml recombinant human TGFβ1 (R&D system). C646 dissolved in DMSO was treated at a dose of 20 μM for 24 hours of pretreatment before TGFβ1 stimulation ...
• Sox2 controls neural stem cell self-renewal through a Fos-centered gene regulatory network

  Miriam Pagin, et al., bioRxiv - Genetics 2021

  Quote: ... Coverslips were incubated with the primary anti-SOX2 antibody (mouse monoclonal IgG2a, 1:100, R&D Systems), overnight at 4°C ...
• Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence

  Mariana De Niz, et al., bioRxiv - Microbiology 2021

  Quote: ... Eph-B4 (R&D systems, used at 20 μg), or VEGFR3 (R&D systems ...
• Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence

  Mariana De Niz, et al., bioRxiv - Microbiology 2021

  Quote: ... or VEGFR3 (R&D systems, used at 20 μg). A temporary glass window (Merk rectangular coverglass ...
• Human cytomegalovirus blocks canonical TGFβ signaling during lytic infection to limit induction of type I interferons

  Andrew H. Pham, et al., bioRxiv - Microbiology 2021

  Quote: ... IFNβ was quantified using human IFN-beta Quantikine ELISA kit (R&D Systems). All measurements were made following the manufacturers’ protocols.
• Complement Decay-Accelerating Factor is a modulator of influenza A virus lung immunopathology

  Nuno Brito Santos, et al., bioRxiv - Microbiology 2021

  Quote: ... cells were lysed with lysis buffer 17 (R&D Systems, 895943) and protein quantified using bicinchoninic acid protein assay (BCA ...
• Complement Decay-Accelerating Factor is a modulator of influenza A virus lung immunopathology

  Nuno Brito Santos, et al., bioRxiv - Microbiology 2021

  Quote: ... After centrifugation, supernatants were used for ELISA analysis (Mouse C3a (TECOmedical, TE1038) or Mouse IFN-γ DuoSet ELISA (R&D Systems, DY485)) ...
• Measles virus exits human airway epithelia via infectious center sloughing

  Camilla E. Hippee, et al., bioRxiv - Microbiology 2021

  Quote: ... Primary antibodies for cleaved caspase-3 (catalog no. MAB835; R&D Systems) and polyclonal rabbit anti-N505 [55] were used at a concentration of 1:800 and 1:1000 respectively ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... Bacterial cells were then incubated with 0.25 μg of purified human SP-D (R&D Systems, 1920-SP-050), ZAG (R&D Systems ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... AnxA1 (R&D Systems, 3770-AN-050) or LPO (MyBiosource ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... ZAG (R&D Systems, 4764-ZA-050), AnxA1 (R&D Systems ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... Antibodies for western blot assays were used at the following concentrations: anti-SP-D (1:2,000, R&D Systems, AF1920), anti-LPO (1:2000 ...
• Proteomic interrogation of the pathogen-host interface in cholera

  Abdelrahim Zoued, et al., bioRxiv - Microbiology 2021

  Quote: ... Bacterial suspensions were incubated with human SP-D at a concentration of 10mg/ml (R&D Systems, 1920-SP-050) for 1h at RT without agitation and observed by light microscopy (Nikon Ti Eclipse equipped with a metal-oxide-semiconductor (sCMOS ...
• HIV-1 hijacks the cell extracellular matrix to spread collectively and efficiently between T lymphocytes

  C. Inizan, et al., bioRxiv - Microbiology 2021

  Quote: ... Mouse IgG2a mAb to CD45 (GAP 8.3) was from ATCC and mouse anti-CD98 mAb was from R&D Systems. Rabbit antibodies (Ab ...
• HIV-1 hijacks the cell extracellular matrix to spread collectively and efficiently between T lymphocytes

  C. Inizan, et al., bioRxiv - Microbiology 2021

  Quote: ... and incubated for 40 min in wells coated with fibronectin (R&D systems). After several washes ...
• HIV-1 hijacks the cell extracellular matrix to spread collectively and efficiently between T lymphocytes

  C. Inizan, et al., bioRxiv - Microbiology 2021

  Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
• Aire-dependent transcripts escape H3K36me3 and Raver2 induced alternative splicing to sustain central immune tolerance

  Francine Padonou, et al., bioRxiv - Immunology 2021

  Quote: ... aprotinin (500 U/mL) and RankL (80 ng/mL) (R&D Systems).
• Aire-dependent transcripts escape H3K36me3 and Raver2 induced alternative splicing to sustain central immune tolerance

  Francine Padonou, et al., bioRxiv - Immunology 2021

  Quote: ... and TGF-β (1 ng/mL) (R&D Systems). The medium 1 was changed every day ...
• miR-573 rescues endothelial dysfunction during dengue infection under PPARγ regulation

  Shefali Banerjee, et al., bioRxiv - Microbiology 2021

  Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
• Lack of blocking activity in anti-CTLA-4 antibodies reduces toxicity, but not anti-tumor efficacy

  Erica L. Stone, et al., bioRxiv - Immunology 2021

  Quote: ... were coated with 0.5 μg/mL rhCTLA-4-Fc chimera (7268-CT, R&D Systems, Minneapolis, MN, USA) diluted in 1× PBS pH 7.0 and incubated at 2-8°C overnight ...
• Conserved epigenetic programming and enhanced heme metabolism drive memory B cell reactivation

  Madeline J. Price, et al., bioRxiv - Immunology 2021

  Quote: ... and stimulated with 500 ng/ml soluble CD40L (R&D Systems, 8230-CL), 10ng/ml IL-4 (R&D Systems ...
• Conserved epigenetic programming and enhanced heme metabolism drive memory B cell reactivation

  Madeline J. Price, et al., bioRxiv - Immunology 2021

  Quote: ... 10ng/ml IL-4 (R&D Systems, 404-ML), and 10 ng/ml IL-5 in complete RPMI ...
• FKBPL and FKBP8 regulate DLK degradation and neuronal responses to axon injury

  Bohm Lee, et al., bioRxiv - Neuroscience 2021

  Quote: ... pan-caspase inhibitor (R&D systems, FMK001) at 10 and 50 μM ...
• Desmosomes polarize mechanical signaling to govern epidermal tissue form and function

  Joshua A. Broussard, et al., bioRxiv - Cell Biology 2021

  Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...