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Citations for R&D Systems :
601 - 650 of 739 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... HEK-293 cells 72h posttransfection or adult DRG neurons at DIV 3 were incubated with commercial anti-CASPR2 antibodies (AF5145, 1:250, R&D Systems, Minneapolis, MN, US) and/or human patient serum (1:50-1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng/mL FGF1 and 5 μg/mL heparin or 10 ng/mL PDGF-AA (R&D Systems #1055-AA-050) supplemented DMEM for 12 hrs.
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bioRxiv - Developmental Biology 2020Quote: ... Micromass cultures were then maintained at 37°C in a humidified incubator with 5% CO2 for 24hrs with the inhibitors before treatment with 5ng TGFβ1/ml (R&D Systems) for the indicated times.
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bioRxiv - Neuroscience 2020Quote: ... OPCs were treated with LCN2 (1, 2.5, 5, 10, 20, 40, 80, or 160 ng/ml, R&D Systems, Shanghai, China) or vehicle control (0.0002% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with increasing doses of TGFβ2 (vehicle control, 1.25 ng/ml, 2.5 ng/ml, and 5 ng/ml; R&D Systems, Minneapolis, MN) for 48 h ...
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bioRxiv - Microbiology 2023Quote: ... 100 µg/ml streptomycin and either 5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Abingdon, UK) to promote M1-differentiation or 20 ng/ml macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Bioengineering 2024Quote: ... 5% CO2 in R10 containing 60 IU mL-1 recombinant human IL-2 (R&D Systems, CAT#202-IL-059/CF). The following day ...
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bioRxiv - Immunology 2024Quote: ... 5×104 labeled cells were resuspended in B cell media (RPMI, 5%FCS, 50μM 2-mercaptoethanol and 2mM glutamine) and stimulated with 50ng/ml CD40L (R&D Systems), 50ng/ml IL-4 (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... , and 5 uM of PfHEUL (PF3D7_0826100) HECT domain were incubated with 10 mM ATP in ubiquitin conjugation reaction buffer (R&D systems). All reactions were incubated at 37C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 1 ng/mL transforming growth factor beta-1 (TGF-β1) and 5 ng/mL fibroblast growth factor-2 (FGF-2) (both from R&D Systems) at 37°C and 5% CO2 in a humidified incubator (Thermo Scientific Heraeus ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µm sections were deparaffinized and stained with either hematoxylin and eosin or with specific antibodies for PROX1 (R&D Systems, AF2727) and GFP (Abcam ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Microbiology 2020Quote: ... fragments were washed three times in 5 ml cold AdDF+++ before being embedded in 30 μL BME (Type 2; R&D Systems) per well in a 48-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... a total of 1 μg native proMMP2 and 0.5 μg proMMP14 were separately incubated in 10 μl with a range of furin (catalog no. 1503-SE-010, R&D systems) concentrations (0 – 250 nM ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2020Quote: ... cells were preincubated for 5 min at room temperature with 1 µg of the unlabeled PS-binding proteins MFG-E8 (R&D systems) and AnV (Biolegend) ...