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Citations for R&D Systems :
501 - 550 of 739 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... following 5 min of stimulation with 10 ng/ml PDGF-BB (R&D Systems, Minneapolis, MN, USA) in the case of the PDGFRαV1/βV2 heterodimer cell line ...
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bioRxiv - Cell Biology 2023Quote: ... the pro- and undifferentiated spermatogonia marker PLZF (day 5-35; 0.6 µg/ml, R&D Systems AF2944), and dual stained with the transcription factor marker FOXO1 (day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with either DMSO or 5 µM Idasanutlin (R&D Systems, #12-35-22-07) for an additional 24 h before cells were harvested for protein and RNA extraction.
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bioRxiv - Developmental Biology 2023Quote: ... transfected cells were provided with 5 ng recombinant mouse TGFβ1 protein (R&D Systems, 7666-MB-005). mCherry fluorescence was determined first by exciting at 570 nm and measuring emission at 610 nm with a 100 msec time delay using a Perkin-Elmer EnVision Xcite Multilabel Plate Reader ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were treated with 100 ng/mL mouse IFNγ or 5 U/mL IFNα (R&D Systems) for 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... heating at 95°C for 5 min in antigen retrieval solution (CTS045, R&D Systems, Minneapolis, MN). After blocking non-specific binding in 2% non-fat milk in 0.1 M PBS containing 0.1% Triton X-100 (PBS/MT ...
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bioRxiv - Immunology 2024Quote: ... dams were treated with 5 μg recombinant murine IL-10 (R&D Systems 417-ML-025/CF) resuspended in sterile PBS ...
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bioRxiv - Immunology 2024Quote: Plates were centrifuged at 825xg for 5 min and supernatants collected for IFNγ ELISA (R&D Systems Mouse IFNγ DuoSet ELISA Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Using a final concentration of 5 ng/mL TGF-β (cat. no. 7666-MB; R&D Systems), cells were incubated for 36h before harvesting ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... TNF-α and MIF were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells and monocytes by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ROCK inhibitor was removed from the medium 3 hours after seeding and cells were induced the following day with 50 ng/ml BMP4 (R&D Systems), 2 μM IWP2 (Stemgent) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Immunology 2022Quote: ... PBMCs from healthy individuals were isolated and expanded in vitro for 7 days in the presence of 50 ng/ml of OKT-3 (Miltenyi) and 600 IU/ml IL-2 (R&D Systems) in AIM-V (Gibco ...
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bioRxiv - Immunology 2020Quote: ... supernatants were collected and secretion of TGF β and MMP2/3/9 was assessed in the supernatant by TGFβ1 ELISA kit (R&D systems) or MMP2/3/9 luminex kit (eBiosciences ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Microbiology 2020Quote: ... hESCs were cultured to achieve 80-90% confluency, and treated with 3 μM CHIR99021 (CHIR, Stem-RD) and 100 ng/ml Activin A (R&D systems) in basal medium RPMI1640 (Cellgro ...
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bioRxiv - Systems Biology 2021Quote: ... HGF 50 ng/ml; NT-3 10 ng/ml; PDGF-BB 10 ng/ml; GDNF 10 ng/ml; All from R&D systems). Ligand treatment was started at the time of tetracycline induction ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative cytokine content of the decellularized mouse heart tissues (n=3) were obtained using the Mouse XL Cytokine Array Kit (ARY028, R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Pathology 2020Quote: ... was measured immediately before injection and at 3 days after injection using a Mouse Lipocalin-2/NGAL Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems) to ensure successful injection of folic acid ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Biochemistry 2020Quote: ... and furin (3 nM) in the presence of the fluorogenic substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog no. ES010, R&D systems). The final substrate concentration was 10 μM in 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC at 60-70% confluence were treated for 3 days with 100 ng/mL Activin A and 100 ng/mL bFGF (R&D Systems) in the presence of increasing levels of FBS (0% on day 1 ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... one million cells were suspended in 100 µl of 1:1 RPMI and BME-Type 3 (R&D Systems, # 36-320-1002P) per implantation ...
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bioRxiv - Biochemistry 2021Quote: ... Test wells were coated with 5 µg recombinant spike protein (rSP; S1+S2; R&D Systems, Minneapolis, MN)/ml coating buffer (0.05 M sodium bicarbonate ...
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bioRxiv - Cell Biology 2020Quote: Mature adipocytes were cultured in complete medium supplemented with AZA (R&D Systems; 5, 10, 20µM; 2 days) and rhPDGF-AB (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were cultured in R10 media containing 5 ng/mL IL-7 (R&D systems). C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Microvessels with and without 24 h treatment with 5 ng mL-1 human recombinant TNFα (R&D Systems), were perfused with THP1s under low shear stress (∼0.2 dyne cm-2 ...
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bioRxiv - Bioengineering 2021Quote: Cells were treated with brefeldin A (10 μg ml-1, R&D Systems, 1231/5 in differentiation media) for 2 h prior to fixation by immersion in paraformaldehyde (4% [v/v] in 1 × PBS ...