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Citations for R&D Systems :
551 - 600 of 884 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Tissue was permeabilized with 0.3% Triton X and quenching and blocking was performed using a cell and tissue staining kit (R&D Systems) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were starved in medium containing 0.1% FBS (mesenchymal stem cell-qualified) for 24 hours and treated with 10 ng/ml PDGF-BB (R&D systems) for 10-15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were trypsinized and incubated with APC-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Systems Biology 2019Quote: ... CCL2 and CCL5 secreted protein levels in cell supernatants from 1×106 murine BMDM’s was performed by ELISA (R&D Systems) according to manufactureŕs instructions.
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bioRxiv - Genomics 2019Quote: 8-week iEPs were stained with mouse E-cadherin-APC antibody (10 μL per one million cells, R&D Systems, FAB7481A) and sorted on a modified Beckman Coulter MoFlo into EcadHigh and EcadLow populations ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (Cell Signaling; #5679S, western blot dilution 1:1000, IHC dilution 1:100; R&D Systems; AF2517, IF dilution 1:100), NR5A2 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: CCL-149 rat lung epithelial cells (ATCC) were cultured in DMEM/F12 with 10% fetal bovine serum (FBS, R&D Systems) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Bioengineering 2020Quote: Expression levels of cell secreted Osteoprotegerin and Vascular Endothelial Growth Factor (VEGF) was examined via ELISA (R&D Systems, Minnesota, USA). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were encapsulated (5·106 cells/mL) in situ within hydrogels loaded with 200 pmol/mL VEGF-165 (R&D Systems) or within non-loaded hydrogels (without GF) ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2020Quote: ... Death-related proteins were measured in cell lysates by a solid-phase antibody array with chemiluminescent detection (R&D Systems, 893900).
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bioRxiv - Cell Biology 2021Quote: ... limiting dilutions of LSK cells and their respective replicate wells were subjected to methylcellulose colony assay with HSC007 (R&D Systems) at 37°C ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were seeded on Matrigel coated culture plates in mTeSR supplemented with 10µM Y-27632 dihydrochloride (R&D Systems, 1254/10) at a density of 56,000/cm2 for routine maintenance of iPSC lines ...
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bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... cells were divided and incubated in the presence or absence of 125 ng/ml recombinant TNF (R&D Systems, Minneapolis, MN) for 24 additional hours ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were treated on either brain or vascular channel with TNF-α (Tumor Necrosis Factor-α, R&D Systems; 210-TA). The treatment was initiated after the formation of a confluent monolayer at ∼5 days in culture ...
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bioRxiv - Immunology 2020Quote: Primary murine T cells were grown in R10 media - RPMI plus 10% fetal bovine serum (FBS; R&D Systems, Minneapolis, MN) supplemented with GlutaMAX (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... the cell populations with a higher ACE2 expression in clone 43.20 were FACS sorted using ACE2-specific antibody (AF933, R&D Systems). After puromycin selection ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged and resuspended in mTeSR1 supplemented with 10 μM SB431542 (SB; Stemgent) and 1 μM Dorsomorphin (R&D Systems). 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm ...
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bioRxiv - Neuroscience 2021Quote: Single-cell RNA-seq analysis of cultured NSCs isolated from the cortex of E14.5 Sprague-Dawley rats (NSC001, R&D systems; cultured according to the manufactures’ protocol ...
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bioRxiv - Microbiology 2020Quote: HTBE and A549 cells were seeded overnight and then treated with 100 IU/ml universal interferon beta (IFN, R&D Systems), or left untreated ...
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bioRxiv - Immunology 2021Quote: ... In-Cell ELISA was then developed using DY999 substrate solution according to the manufacturer’s recommendations (R&D Systems, Minneapolis, MN USA) and measured at 450 nm (and at 630 nm to assess the background ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were released from plates by incubating with 0.05% trypsin and then stained with primary antibody (1 μg/106 live cells) against NPY1R (MAB6400; R&D Systems) followed by secondary antibody against human IgG for 30 minutes on ice and/or with PE-conjugated ABCC5 (sc-376965 PE ...
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bioRxiv - Immunology 2022Quote: ... BAL cells were treated for 2h before infection with an ACE2 blocking antibody at 10 μg/ml (AF933, R&D systems). SARS-CoV-2 was added to the cells at MOI 0.1 or 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Macrophage differentiation from bone marrow cells was obtained by culture in standard growth medium supplemented with m-CSF (R&D Systems) at 30 ng/ml for 7 days.
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bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems) and StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR analysis of pluripotency markers was done using the Human Pluripotent Stem Cell Assessment Primer Pair Panel (R&D Systems, SC012) and amplification was done using GoTaq Green ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Molecular Biology 2020Quote: Cytokine levels in cell culture supernatants samples were determined using the Proteome Profiler Mouse Cytokine Array Panel A (R&D Systems) following the manufacturer recommendations.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in PBS and stained with a goat anti-human ACE2 polyclonal antibody (R&D Systems, Cat # AF933) or isotype goat polyclonal isotype control (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Canada.70 Cells were maintained in DMEM supplemented with 10% fetal bovine serum (TET tested, heat inactivated, R&D SYSTEMS INC), 1% antibiotic-antimycotic ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from CH12 cells cultured for 3 days with TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were activated in the presence of the following sets of cytokines and/or neutralizing antibodies (all from R&D Systems): Th0—media alone ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Neuroscience 2020Quote: ... Myeloid progenitor cells were treated in suspension for 1 h with 5μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920 ...
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bioRxiv - Cancer Biology 2022Quote: ... 300 μg cell lysates were used for phosphorylation analysis with Human Phospho-Kinase Array Kit (ARY003B, R&D Systems, Minneapolis, MN) following the manufacturer’s instruction.
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bioRxiv - Cell Biology 2022Quote: ... the cells were treated in the presence or absence of interferon γ (IFN-γ 25ng/ml (R&D Systems, 285-IF) for 24 hours ...
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bioRxiv - Genetics 2022Quote: Supernatants from transfected HEK293T cells were analyzed for their LBP content by Human LBP DuoSet ELISA (#DY870-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... During this time cells were cultured in MEF-conditioned medium (70% MEF-conditioned medium, 30% TSC medium, FGF4 (37.5 ng/ml, R&D System), Heparin (1.5 μg/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fixed cells were stained with 1:100 dilution of APC-conjugated anti-SOX-17 (R&D Systems, Minneapolis, MN, USA; IC1924A) for 30 mins at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were resuspended in an antibody solution containing 5μL DYKDDDDK Epitope Tag Alexa Fluor 647-conjugated Antibody (R&D Systems #IC8529R) and 45μL DBPS + 10% FBS and incubated in the dark at room temperature for one hour ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).