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Citations for R&D Systems :
501 - 550 of 884 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... was quantified in cell culture media using a SDC4 ELISA as per the manufacturer’s instructions (DY2918, R&D Systems).
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bioRxiv - Cell Biology 2023Quote: ... plated cells were transferred into complete basal media supplemented with 0.05 ng/μL BMP4 (R&D systems, 5020-BP), 20 ng/mL FGF and 1 mg/mL laminin (Martynoga et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with compounds for 2 hours prior to TGF-β1 stimulation (1ng/ml, R&D systems. After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... Adaptive NK cell-positive donors were identified by anal-ysis of NKG2C (FAB138P or FAB138A antibodies, R&D Systems) and FcεRIγ (FCABS400F antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... at 3500 cells per well in GK15 medium supplemented with 200 ng/ml BMP4 (R&D Systems, 314-BP-010), 100 ng/ml SCF (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with PE-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Systems Biology 2021Quote: ... cells were treated with ligand in fresh EM media as follows: 10 ng/ml EGF (R&D Systems #236-EG), 40 ng/ml HGF (R&D Systems #294-HG) ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared and analyzed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, cat# ARY003B) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Immunology 2022Quote: ... cells were washed at day 7 and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 250 U/ml IFN-α (R&D Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
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bioRxiv - Immunology 2022Quote: ... and MC-38 cells were cultured in Dulbecco’s Modified Eagle’s Medium (ATCC) supplemented with 10% fetal bovine serum (FBS, R&D Systems), 100 U/mL penicillin (Thermo) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Immunology 2022Quote: ... were purified from mouse spleens and lymph nodes by negative selection using a T-cell enrichment column (R&D Systems). For the generation of OTI or OTII T blast cells ...
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bioRxiv - Immunology 2019Quote: ... Transduced cells were selected for in the presence of 2 µg/ml puromycin (R&D Systems Inc, Minneapolis, MN, USA), live cells harvested by ficoll gradient and flow cytometry used to determine purity based on expression of eBFP indicative of transduction.
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bioRxiv - Cancer Biology 2019Quote: All the cells used in this study were tested for mycoplasma using Mycoprobe Mycoplasma Detection kit from R&D system. Cells were passaged no more than 10 times from the initial stocks ...
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bioRxiv - Cell Biology 2019Quote: ... Directional cell migration was induced by overlaying the polymerized gels with 0.63μg/ml CCL19 diluted in complete media (R&D Systems). To prevent drying-out of the gels ...
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bioRxiv - Bioengineering 2020Quote: ... the cell culture medium was replaced by adipocyte differentiation medium or osteoblast differentiation medium (StemX-Vivo™, R&D systems). MSC expansion medium was also used as the control condition ...
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bioRxiv - Immunology 2019Quote: ... cells were simulated in R-10 medium in the presence of either 20 ng/mL IL-23 (R&D Systems) or 250 ng/mL PMA (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and IL-10 were measured in cell supernatants using an enzyme-linked immunosorbent assay technique (R&D systems). The sensitivity of the assays was (pg/ml ...
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bioRxiv - Immunology 2020Quote: ... IL-2 production was determined 24 h after T-cell activation by ELISA according to manufacturer’s instructions (R&D Systems). CD69 production was analyzed by flow cytometry after 2 days of anti-CD3 and/or anti-CD28 stimulation.
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bioRxiv - Immunology 2020Quote: Concentrations of IL-6 in cell culture supernatant were determined using the mouse DuoSet kit (R&D Systems, Minneapolis, MN). All assays were performed according the manufacturer’s supplied protocol ...
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bioRxiv - Immunology 2021Quote: IL-6 secretion from RAW264.7 cells was measured using the mouse IL-6 Duoset ELISA kit (R&D systems, UK) according to manufacturer’s procedure.
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bioRxiv - Cell Biology 2022Quote: IL-1β in mice serum and cell-free supernatants were measured by Quantikine® sandwich ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... immune mediators in cell supernatants were measured using Human Custom 29-plex Multi-Analyte Kit (R&D Systems, Minneapolis, Minnesota) measuring the following analytes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were stimulated with a pro-inflammatory cocktail of 20ng/ml interferon-γ (IFNγ, R&D Systems #485-MI-100) and 50ng/ml tumor necrosis factor α (TNFα ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Zoology 2022Quote: ... The transfected cells were cultured in iPSC medium with 200 ng/mL of recombinant viral B18R protein (R&D Systems). B18R was removal once the transfected cells were ready to re-seed onto irradiated MEFs.
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (with RA-containing B27 supplement) with 100 ng/ml rhFGF2 (R&D Systems, #233-FB), 100 ng/ml rhFGF10 (R&D Systems ...
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bioRxiv - Genomics 2022Quote: ... National Cancer Institute Biologics Repository) for T cells and using 10 ng/mL rhTNF-α (210-TA, R&D Systems) for monocytes ...
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bioRxiv - Immunology 2023Quote: ... Cells (2E6 in 25 microliters) were stained for 90 min with anti-mouse S1PR1 (74.4 μg/ml; R&D Systems), washed twice in buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with complete osteogenic medium mixed by human/mouse StemXVivo osteogenic/adipogenic base medium (CCM007, R&D Systems) and mouse StemXVivo ostegenic supplement (CCM009 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were plated into semi-solid methylcellulose (MethoCult™ H4435 Enriched, STEMCELL Technologies or Enriched Human Methylcellulose, R&D Systems), with further addition of 100 pg/ml rh-IL21 ...
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bioRxiv - Immunology 2023Quote: Phosphoprotein expression of CD8+ T cells was detected using a human phospho-kinase array kit (ARY003C, R&D Systems, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Immunology 2023Quote: ... IFN-beta in the cell culture supernatants was determined using the mouse IFN-beta DuoSet ELISA kit (R&D System) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: The ROSAKIT WT human cell line (RRID:CVCL_5G49) was cultured in the presence of recombinant human SCF (80 ng/mL, R&D Systems), as described previously (23) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in cold PBS containing 5.6mg/mL Cultrex Reduced Growth Factor BME (R&D Systems, 3533-010-02). Primary tumor and metastatic tumor burdens were measured weekly for 4 and 6 weeks for tail vein injection models and orthotopic models ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in cell supernatants were assessed using the mouse IL-1β/IL-1F2 DuoSet ELISA kit (R&D Systems) and mouse TNF-α DuoSet ELISA kit (R&D Systems ...
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bioRxiv - Biochemistry 2023Quote: Cell lines were acquired from ATCC (HEK293T, H1299, HT1080) and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: For Wnt5a experiments tetracycline induced GFP-RasV12 cells were treated with 100ng/mL recombinant human/mouse Wnt5a (R&D Systems) or PBS (control-Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... For immunoprecipitation 1mg of pre-cleaned cell lysate was incubated with 4µg mouse monoclonal L1TD1 antibody (R&D systems, MAB8317) at 4 °C at 20rpm on the rotor overnight ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Microbiology 2024Quote: Quantification of cytokines present in cell culture supernatants was performed by ELISA using the DuoSet ELISA kit (R&D Systems) for TNF-α ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Biochemistry 2024Quote: The human cell line H1299 was acquired from ATCC and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...